A simple method for the determination of lipid composition of human bile.

Muraca, Maurizio; Vilei, Maria Teresa; Miconi, L.; Petrin, P; Antoniutti, Michele; Pedrazzoli, Sergio

doi:10.1016/s0022-2275(20)42097-8

Cited by 18 publications

(7 citation statements)

References 10 publications

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“…More than 95% of secreted bile acids are actively absorbed by the terminal ileum and sent back to the liver in a process known as enterohepatic circulation (Di Ciaula et al, 2017). Bile is a highly complex mixture, although bile acids dominate the dry weight of biliary bile (Muraca et al, 1991). Many prior studies have focused on crude extracts from varying sources, including ruminants, containing poorly defined mixtures of bile molecules.…”

Section: A Pipeline For Randomization Of Microbiome Membersmentioning

confidence: 99%

Interpersonal Gut Microbiome Variation Drives Susceptibility and Resistance to Cholera Infection

Alavi

Mitchell

Cho

et al. 2020

Cell

133

140

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Section: A Pipeline For Randomization Of Microbiome Membersmentioning

confidence: 99%

Interpersonal Gut Microbiome Variation Drives Susceptibility and Resistance to Cholera Infection

Alavi

Mitchell

Cho

et al. 2020

Cell

133

140

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“…At T = 24 hr, a biliary sample was obtained in the morning fasting state by use of the Enterotest (HDC Corp). This commercially available device for sampling of gastrointestinal contents was used to determine biliary bile salt and cholesterol contents, as previously described (Muraca et al, 1991;Van der Meer et al, 1990). Participants swallowed the encapsulated nylon thread with water in the evening before the T = 24 hr study visit; one end was taped at a corner of the mouth.…”

Section: Human Subjectsmentioning

confidence: 99%

“…Biliary lipids were extracted according to Bligh and Dyer (1959) from the freshly frozen bile samples and from bile eluted from the Enterotests as previously described (Muraca et al, 1991). Biliary free cholesterol was derivatized using N,O-bis-(trimethylsilyl)trifluoroacetamide and pyridine (1:1) with 1% trimethylchlorosilane at room temperature.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Transintestinal Cholesterol Transport Is Active in Mice and Humans and Controls Ezetimibe-Induced Fecal Neutral Sterol Excretion

et al. 2016

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Except for conversion to bile salts, there is no major cholesterol degradation pathway in mammals. Efficient excretion from the body is therefore a crucial element in cholesterol homeostasis. Yet, the existence and importance of cholesterol degradation pathways in humans is a matter of debate. We quantified cholesterol fluxes in 15 male volunteers using a cholesterol balance approach. Ten participants repeated the protocol after 4 weeks of treatment with ezetimibe, an inhibitor of intestinal and biliary cholesterol absorption. Under basal conditions, about 65% of daily fecal neutral sterol excretion was bile derived, with the remainder being contributed by direct transintestinal cholesterol excretion (TICE). Surprisingly, ezetimibe induced a 4-fold increase in cholesterol elimination via TICE. Mouse studies revealed that most of ezetimibe-induced TICE flux is mediated by the cholesterol transporter Abcg5/Abcg8. In conclusion, TICE is active in humans and may serve as a novel target to stimulate cholesterol elimination in patients at risk for cardiovascular disease.

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“…Docosapentaenoic acid (22:5) and docosahexaenoic acid (22:6) are important omega-6 PUFAs that are precursors to prostaglandins and leukotrienes, important in the inflammatory response. Non-TAG lipids upregulated in the S508A mutant were phospholipids, suggesting that these are the lipids destined for efflux via enlarged BCs, since phospholipids, not TAGs, are a common component of bile ( 47 ).…”

Section: Resultsmentioning

confidence: 99%

Human CEACAM1-LF regulates lipid storage in HepG2 cells via fatty acid transporter CD36

Chean

Chen

Gugiu

et al. 2021

Journal of Biological Chemistry

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show abstract

A simple method for the determination of lipid composition of human bile.

Cited by 18 publications

References 10 publications

Interpersonal Gut Microbiome Variation Drives Susceptibility and Resistance to Cholera Infection

Interpersonal Gut Microbiome Variation Drives Susceptibility and Resistance to Cholera Infection

Transintestinal Cholesterol Transport Is Active in Mice and Humans and Controls Ezetimibe-Induced Fecal Neutral Sterol Excretion

Human CEACAM1-LF regulates lipid storage in HepG2 cells via fatty acid transporter CD36

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