A simple method for the detection and genotyping of high-risk human papillomavirus using seminested polymerase chain reaction and reverse hybridization

Lin, Hao; Moh, Jau-Sung; Ou, Yu‐Che; Shen, Shu-Yun; Tsai, Yi-Min; Changchien, Chan-Chao; Liu, Jacqueline M.; Ma, Yen-Ying

doi:10.1016/j.ygyno.2004.09.043

Cited by 19 publications

(19 citation statements)

References 30 publications

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“…Furthermore, a highly sensitive/specific molecular methodology such as nested PCR followed by capillary sequencing was used [49]. These results confirm the prevalence of HPV-DNA in OSCC and also that HPV 16 is the most frequent genotype.…”

Section: Discussionmentioning

confidence: 59%

Distribution of Human Papillomavirus Genotypes in Sardinian Patients with Oral Squamous Cell Carcinoma~!2010-03-01~!2010-05-10~!2010-07-13~!

Montaldo¹,

Mastinu²,

Zorco³

et al. 2010

TOVJ

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Human papillomaviruses (HPVs) seem to play an important role in the pathogenesis of gynecological carcinomas and in head and neck carcinomas. The aim of this study was to detect and genotype HPVs in fresh oral squamous cell carcinoma (OSCC) from a Sardinian population, and to determine whether HPV presence was significantly associated with the development of OSCC.The oral mucosa tissues were obtained from 120 samples (68 OSCC and 52 control samples) taken from a Sardinian population seen at the Dental Clinic of the Department of Surgery and Odontostomatological Sciences, University of Cagliari (Italy) and the “ Ospedale SS Trinità”, Cagliari (A.S.L. 8) between 2007 and 2008. PCR was used for the detection of HPV DNA and the genotype was determined by DNA sequencing. The frequency of HPV infection was evaluated in relation to age, sex, smoking and alcohol use. Statistical analysis was performed using the SPSS 11.5 software.The results showed the presence of HPV-DNA in 60.3% of OSCC with HPV-16 (51.2%) being the most frequent genotype. In these Sardinian OSCC patients, HPV-DNA was detected more in males (65.8%) than in females (34.1%) while controls show a 0% of HPV presence. HPV positive was highly associated with OSCC among subjects with a history of heavy tobacco and alcohol use and among those with no such history.A greater frequency of high risk HPV presence was observed in patients with OSCC compared to health control patients. In addition these results suggested that oral HPV presence could be associated in OSCC subjects. Our results need more analyses to detect the HPV-DNA integration into tumoral cells.

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Section: Discussionmentioning

confidence: 59%

Distribution of Human Papillomavirus Genotypes in Sardinian Patients with Oral Squamous Cell Carcinoma~!2010-03-01~!2010-05-10~!2010-07-13~!

Montaldo¹,

Mastinu²,

Zorco³

et al. 2010

TOVJ

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“…Seminested polymerase chain reaction (PCR) and reverse hybridization were used for HPV typing. 24 Quantitative methylation-specific PCR was used for DNA methylation tests. Briefly, 500-ng genomic DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold Kits (Zymo Research, Irvine, Calif ), according to the manufacturer's recommendations.…”

Section: Dna Preparation Hpv Tests and Dna Methylationmentioning

confidence: 99%

Atl

Kan

Liou

Wang

et al. 2014

Int J Gynecol Cancer

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“…The concentration and quality of extracted DNA were determined by a spectrophotometer (Biochrom, S2100 Diode Array). The purified DNA was tested suitable for PCR by using GH20/PC04 primers for human β-globin gene [13]. All the cases were screened by uniplex PCR for GBS identification using GBS-specific primers.…”

Section: Methodsmentioning

confidence: 99%

Epidemiology of Group B <b><i>Streptococcus</i></b> ST-17 Clone in Pregnant Women of South Taiwan

Hsu

Shen³

et al. 2012

Gynecol Obstet Invest

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Objectives: We aimed to utilize a simple molecular assay to simultaneously detect both group B Streptococcus (GBS) and virulent ST-17 rectovaginal colonization. We also attempted to estimate the prevalence of maternal GBS and ST-17 carriers and to evaluate their seasonal association. Subjects and Methods: We used an optimized multiplex PCR method employing scp-B and ST-17 primers to analyze DNA extracted from rectovaginal swabs of 3,064 cases collected over 3 years. The incidence trends, seasonal variations, and temperature preference were analyzed. Results: The overall prevalence of maternal colonization for GBS and ST-17 clone were 13.25 and 2.48%, respectively. The ST-17 to GBS ratio was 18.72%. The occurrence of ST-17 colonization was significantly associated with seasonal variations with a preference for lower temperatures. Conclusions: We developed a novel multiplex PCR method suitable for the simultaneous detection of GBS and ST-17 clone. The phenomenon of lower temperature preference for ST-17 clone necessitates further investigation. The epidemiological data for GBS and ST-17 incidence are especially important to establish a public policy for universal GBS screening in the future.

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A simple method for the detection and genotyping of high-risk human papillomavirus using seminested polymerase chain reaction and reverse hybridization

Cited by 19 publications

References 30 publications

Distribution of Human Papillomavirus Genotypes in Sardinian Patients with Oral Squamous Cell Carcinoma~!2010-03-01~!2010-05-10~!2010-07-13~!

Distribution of Human Papillomavirus Genotypes in Sardinian Patients with Oral Squamous Cell Carcinoma~!2010-03-01~!2010-05-10~!2010-07-13~!

Atl

Epidemiology of Group B <b><i>Streptococcus</i></b> ST-17 Clone in Pregnant Women of South Taiwan

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