A simple method to measure drug effects on human sperm motility.

Hong, Chuang‐Ye; Saintonge, D M Chaput de; Turner, Paul

doi:10.1111/j.1365-2125.1981.tb01137.x

Cited by 75 publications

(38 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This inhibition was completely reversible by 100-fold dilution with fresh reactivating solution containing no lithium. The trans-membrane migration ratio (TMMR) method (Hong et al, 1981) has been found to relate closely to visual methods for assessing sperm motility (Lee et al, 1989). Using the original method it was found that lithium inhibited sperm motility, the concentration inhibiting sjperm motility by 50% (EC50) being 63 mmol I-) (Grech et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Lithium inhibits human sperm motility in vitro.

Raoof

Pearson

Turner

1989

Brit J Clinical Pharma

Self Cite

View full text Add to dashboard Cite

Lithium is a widely prescribed drug used for the treatment of bipolar affective illness. Previous reports on its effects on sperm motility and male fertility are conflicting. The effect of lithium on human sperm motility was examined in vitro using the modified transmembrane migration method. This technique takes account of the dilution of lithium that occurs during the incubation. Lithium inhibits human sperm motility in vitro in concentrations comparable with those reported to be achieved in semen after oral administration.

show abstract

Section: Introductionmentioning

confidence: 99%

Lithium inhibits human sperm motility in vitro.

Raoof

Pearson

Turner

1989

Brit J Clinical Pharma

Self Cite

View full text Add to dashboard Cite

show abstract

“…Only samples with sperm concentrations higher than 15 x 10' ml-and percentage of progressive forward moving sperms higher than 20% were used because a previous study (Hong et al, 1981a) had shown that motility measurement was less accurate in poor motility samples All drugs were dissolved in phosphate buffered saline at pH 7.3 (Dulbecco 'A', Oxoid Ltd). Each semen sample was divided into several 100,ul aliquots which were then mixed with 50 ,1 of drug solution or phosphate buffer.…”

Section: Methodsmentioning

confidence: 99%

“…Determination of sperm motility was carried out as previously described (Hong et al, 1981a). 100 ,lI of semen-buffer or semen-drug mixture was pipetted into a small tube to the lower end of which a sheet of Nuclepore membrane with many evenly distributed 5…”

Section: Methodsmentioning

confidence: 99%

Influence of lipid solubility on the sperm immobilizing effect of beta‐ adrenoceptor blocking drugs.

Hong¹,

Turner²

1982

Brit J Clinical Pharma

Self Cite

View full text Add to dashboard Cite

I Sperm immobilizing effects of five ,3-adrenoceptor blockers were measured on six human semen samples with a trans-membrane migration method.2 The concentrations which decreased sperm motility to 50% of control (ED50) were 0.8, 4.2, 6.2, 11.0 and 33.0 mm for propranolol, oxprenolol, metoprolol, acebutolol and sotalol respectively. 3 There was a linear correlation between the log values of ED50 and the log partition coefficients of these ,3-adrenoceptor blockers. 4 Because the lipid solubility determines the sperm immobilizing potencies of 83-adrenoceptor blockers, we support the theory that ,8-adrenoceptor blockers inhibit sperm motility by stabilizing the cellular membrane. 5 The clinical implication of this finding is that more lipid soluble drugs are likely to have more interference on sperm function, at least when they are used locally.

show abstract

“…The transmembrane migration ratio (TMMR) method has been used to measure the effect of various pharmacological agents on sperm motility (Hong et al, 1981a). It is objective, simple, quantitative, reproducible and requires only a small volume of semen (100 ,ul) for each test which allows replicate testing in the same sample.…”

Section: Introductionmentioning

confidence: 99%