A simple method to stimulate ischemic stroke in Wistar rat for animal testing

Adnyana, Made Oka; Sudewi, Anak Agung Raka; Samatra, Dewa Putu Gde Purwa; Suprapta, Dewa Ngurah; Aulanni’am, Aulanni’am

doi:10.15562/bmj.v6i1.430

Cited by 4 publications

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“…All animal models were previously anaesthetised using 10 mg/kg body weight of ketamine given intravenously via caudal vein. Ischemic stroke upon Wistar rats was induced by the previously described method [11], i.e. by ligating the right common carotid artery, right external carotid artery, and internal carotid artery on its root to the middle cerebral artery using silk one med soak for 2 hours.…”

Section: Methodsmentioning

confidence: 99%

Balinese Cultivar of Purple Sweet Potato Improved Neurological Score and BDNF and Reduced Caspase-Independent Apoptosis among Wistar Rats with Ischemic Stroke

Adnyana¹,

Sudewi²,

Samatra³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND:Ischemic stroke occurs due to the abrupt occlusion in the brain which leads to neuronal death. Neuronal death in ischemic stroke is due to increase production of reactive oxygen species (ROS). Neuronal death occurs via necrosis and apoptosis mechanisms. Apoptosis can either occur via extrinsic or intrinsic pathway. Meanwhile, the intrinsic pathway can be caspase-dependent or independent. Anthocyanin is a natural pigment with antioxidant, anti-inflammatory, anti-cancer, and neuroprotective properties. Balinese cultivate of purple potato extract contains a high level of anthocyanin and has been proven for its antioxidant activity.AIM:Antioxidant effect of Balinese cultivates purple potato extract has not been studied on an animal model with ischemic stroke. Accordingly, we would like to study the effect of antioxidant properties from Balinese cultivate of purple potato extract by assessing the neurological score, BNDF concentration, and caspase-independent apoptosis by measuring AIF concentration on Wistar rats with ischemic stroke.METHODS:This was an experimental study using male Wistar rats age between 12-14 weeks weigh between 200 to 250 g.RESULTS:This study demonstrated a significant difference of neurological score on day 3 among control versus treatment groups. Balinese cultivate of purple potato extract markedly reduced AIF, increased BDNF, and suppressed apoptosis among treatment group when compared with the control group.CONCLUSION:We have proven the efficacy of antioxidant activity of anthocyanin derived from Balinese cultivar of purple sweet potato by elevated AIF levels, lower apoptosis rate, improved neurological score on day-3 to day-7 post-stroke, as well as increased BDNF levels.

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Section: Methodsmentioning

confidence: 99%

Balinese Cultivar of Purple Sweet Potato Improved Neurological Score and BDNF and Reduced Caspase-Independent Apoptosis among Wistar Rats with Ischemic Stroke

Adnyana¹,

Sudewi²,

Samatra³

et al. 2019

Open Access Maced J Med Sci

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“…Rats were fed regularly, a night before the ischemic stroke induction protocol. In the morning, ischemic stroke was inducted according to previous methods [ 18 ]. A total of 20 rats were used for the study, each of which was 10 for the control and treatment group.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Effects of Purple Sweet Potato Balinese Cultivar in Wistar Rats With Ischemic Stroke

Adnyana

Sudewi

Samatra

et al. 2018

Open Access Maced J Med Sci

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BACKGROUND:Purple sweet potato (Ipomoea Batatas L.) is one of the sources for anthocyanin, which promotes the health through antioxidant, anti-inflammatory, anti-cancer, neuroprotection, and anti-apoptosis activities. Oxidative stress has been shown to be the cause of apoptosis in ischemic stroke.AIM:The objective of this research was to delineate the pleiotropic effects of anthocyanin for neuroprotection during an acute stroke event.METHODS:Anthocyanin was extracted from Balinese cultivar of purple sweet potato and subsequently administered to rat models of induced ischemic stroke (labelled as treatment group), as well as a placebo (labelled as a control group). Several parameters were in turn evaluated, i.e. the activities of anti-apoptotic (Bcl-2) as well as pro-apoptotic (cytochrome c, caspase-3) molecules, and apoptosis rate. Bcl-2 levels were determined using the histochemical method, cytochrome c and caspase-3 via ELISA method, while apoptosis rate was measured by TdT-medicated Dutp-Nick End Labeling (TUNEL) assay.RESULTS:Bcl-2 expression demonstrated significantly higher Bcl-2 expression in the treatment compared with control group (median 31.2 vs. 1.1; p = 0.001). Accordingly, pro-apoptotic cytochrome c and caspase-3 levels were also found significantly lower in the treatment as opposed to control group (mean 4.17 vs. 8.06; p = 0.001; mean 3.81 vs. 8.02; p = 0.001). Ultimately, apoptosis rate was found markedly lower among treatment than control groups (mean 3.81 vs. control 21.97; p = 0.003).CONCLUSION:The results of this study indicated a significant neuroprotective effect of anthocyanin derived from Balinese cultivar of PSP. Anthocyanin was able to increase and reduce anti-apoptotic and pro-apoptotic protein levels, respectively, resulting in lesser cellular apoptotic rate when compared with placebo. The potential mechanism was thought mainly due to its anti-oxidant properties.

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“…The ligation of the middle cerebral artery was conducted based on the conventional method with slight modification [17].This method was done by ligating the brain artery in the Common Carotid Artery (CCA), External Carotid Artery (ECA) and Internal Carotid Artery (ICA). The occlusion was done for 3 h. After 3 h, the ligation was released for reperfusion.…”

Section: Middle Cerebral Artery Occlusion (Mcao) Techniquementioning

confidence: 99%

Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

Wati

Prasetyawan

Mahdi

et al. 2018

J. Pure App. Chem. Res.

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This paper reports the effect of anthocyanin from purple sweet potato in the cerebellum of ischemic stroke rats model by Middle Cerebral Artery Occlusion (MCAO) technique. This technique was carried out by ligating the blood flow in External Carotid Artery (ECA), Common Carotid Artery (CCA) and Internal Carotid Artery (ICA) for 3 hours, followed by reperfusion. The MCAO technique proved a technique for preparing ischemic stroke rats. This technique induces releasing of Brain-Derived Nuclear Factor (BDNF) and Vascular Endothelial Growth Factor (VEGF) in the cerebellum of ischemic stroke rats. The levels of BDNF measured by ELISA technique and VEGF expression used immunohistochemistry technique. The results showed that anthocyanin from purple sweet potato increases the level of both BDNF and VEGF expression in the cerebellum of ischemic stroke rats. It is suggested that anthocyanin could be used as a therapeutic agent for ischemic stroke.

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A simple method to stimulate ischemic stroke in Wistar rat for animal testing

Cited by 4 publications

References 0 publications

Balinese Cultivar of Purple Sweet Potato Improved Neurological Score and BDNF and Reduced Caspase-Independent Apoptosis among Wistar Rats with Ischemic Stroke

Balinese Cultivar of Purple Sweet Potato Improved Neurological Score and BDNF and Reduced Caspase-Independent Apoptosis among Wistar Rats with Ischemic Stroke

Neuroprotective Effects of Purple Sweet Potato Balinese Cultivar in Wistar Rats With Ischemic Stroke

Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

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