A simple method to study motor and non-motor behaviors in adult zebrafish

Selvaraj, Velanganni; Venkatasubramanian, Hemagowri; Ilango, K.; Santhakumar, Kirankumar

doi:10.1016/j.jneumeth.2019.03.008

Cited by 13 publications

(17 citation statements)

References 47 publications

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“…In terms of locomotion, zebrafish exhibit sophisticated behaviours reflective of PD. A number of studies have reported the effects of MPTP on zebrafish locomotion [ 18 , 19 , 21 , 25 , 30 ], but only a few have performed prolonged observation [ 21 , 25 ]. Assessing the behavioural changes at several time points is critical as it provides stronger evidence for the establishment of MPTP-induced zebrafish model of PD.…”

Section: Discussionmentioning

confidence: 99%

“…It has the ability to mimic the specific degeneration of nigral dopaminergic neurons seen in the development of PD. According to Sarath Babu et al [ 18 ] and Selvaraj et al [ 19 ], reduced locomotor activity in MPTP-induced animal models is comparable to human bradykinesia and dyskinesia, suggesting a disruption in motor control ability.…”

Section: Discussionmentioning

confidence: 99%

“…Evidently, the effect of MPTP can be seen one day after the injection [ 18 , 19 ]. Even though MPTP has been used to induce PD in zebrafish, findings are frequently limited to a single time point (24 hours post-injection).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model: A 96-hour behavioral study

et al. 2022

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Parkinson’s disease (PD) is the most common brain motor disorder, characterized by a substantial loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Motor impairments, such as dyskinesia, bradykinesia, and resting tremors, are the hallmarks of PD. Despite ongoing research, the exact PD pathogenesis remains elusive due to the disease intricacy and difficulty in conducting human studies. Zebrafish (Danio rerio) has emerged as an ideal model for researching PD pathophysiology. Even though 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been used to induce PD in zebrafish, behavioural findings are frequently limited to a single time point (24 hours post-injection). In this sense, we aim to demonstrate the effects of MPTP on zebrafish swimming behaviour at multiple time points. We administered a single dosage of MPTP (200μg/g bw) via intraperitoneal injection (i/p) and assessed the locomotor activity and swimming pattern at 0h, 24h, and 96h post-injection through an open field test. Analysis of the behaviour revealed significant reductions in swimming velocity (cm/s) and distance travelled (cm), concurrent with an increase in freezing maintenance (duration and bouts) in zebrafish injected with MPTP. In addition, the MPTP-injected zebrafish exhibited complex swimming patterns, as measured by the turn angle, meander, and angular velocity, and showed abnormal swimming phenotypes, including freezing, looping, and erratic movement. To conclude, MPTP administration into adult zebrafish induced hypolocomotion and elicited motor incoordination. Plus, the effects of MPTP were observable 24 hours after the injection and still detectable 96 hours later. These findings contribute to the understanding of MPTP effects on adult zebrafish, particularly in terms of swimming behaviours, and may pave the way for a better understanding of the establishment of PD animal models in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model: A 96-hour behavioral study

et al. 2022

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“…A few of these compounds include deprenyl, melatonin and rosmarinic acid which act to alleviate stress through MAO-B inhibition, anti-oxidation and/or anti-inflammation (Parng et al, 2006;Sallinen et al, 2009;Díaz-Casado et al, 2016;Zhao et al, 2020). In adults, reports primarily consist of behavioral assessment without much examination of dopaminergic neurons and their mitochondria (Flinn et al, 2008;Selvaraj et al, 2019). However of the few that have investigated dopaminergic health, Anichtchik et al (2004) have shown that a systemic injection of MPTP resulted in decreases in dopamine and noradenaline concentrations in the brain without any effect on TH immunoreactivity, TH co-localization with TUNEL or any increase in caspase-3 activity.…”

Section: Discussion the Efficiency Of Cvmi For Dopaminergic Cell Deathmentioning

confidence: 99%

Cerebroventricular Microinjections of MPTP on Adult Zebrafish Induces Dopaminergic Neuronal Death, Mitochondrial Fragmentation, and Sensorimotor Impairments

Kalyn

Ekker

2021

Front. Neurosci.

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Mitochondria are dynamic organelles that mediate the energetic supply to cells and mitigate oxidative stress through the intricate balance of fission and fusion. Mitochondrial dysfunction is a prominent feature within Parkinson disease (PD) etiologies. To date, there have been conflicting studies of neurotoxin impact on dopaminergic cell death, mitochondrial function and behavioral impairment using adult zebrafish. Here, we performed cerebroventricular microinjections (CVMIs) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on adult transgenic zebrafish that resulted in significant reductions in dopaminergic neurons within the telencephalon and olfactory bulbs (OB) of Tg(dat:eGFP) fish. Visualization of mCherry and mitochondrial gene expression analysis in Tg(dat:tom20 MLS:mCherry) fish reveal that MPTP induces mitochondrial fragmentation in dopaminergic neurons and the activation of the pink1/parkin pathway involved mitophagy. Moreover, the loss of dopaminergic neurons translated into a transient locomotor and olfactory phenotype. Taken together, these data can contribute to a better understanding of the mitochondrial impact on dopaminergic survivability.

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“…PD-like locomotor deficits are also well-reported in zebrafish models, observed as shorter distance swum, slower swimming, and frequent freezing bouts [20,21]. Although PD-like motor deficits caused by MPTP have been previously reported in zebrafish [22,23], their potential cognitive phenotypes in this model have not yet been tested.…”

Section: Introductionmentioning

confidence: 99%

MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline

Bashirzade

Чересиз

Belova

et al. 2022

Toxics

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The zebrafish is a promising model species in biomedical research, including neurotoxicology and neuroactive drug screening. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) evokes degeneration of dopaminergic neurons and is commonly used to model Parkinson’s disease (PD) in laboratory animals, including zebrafish. However, cognitive phenotypes in MPTP-evoked experimental PD models remain poorly understood. Here, we established an LD50 (292 mg/kg) for intraperitoneal MPTP administration in adult zebrafish, and report impaired spatial working memory (poorer spontaneous alternation in the Y-maze) in a PD model utilizing fish treated with 200 µg of this agent. In addition to conventional behavioral analyses, we also employed artificial intelligence (AI)-based approaches to independently and without bias characterize MPTP effects on zebrafish behavior during the Y-maze test. These analyses yielded a distinct cluster for 200-μg MPTP (vs. other) groups, suggesting that high-dose MPTP produced distinct, computationally detectable patterns of zebrafish swimming. Collectively, these findings support MPTP treatment in adult zebrafish as a late-stage experimental PD model with overt cognitive phenotypes.

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A simple method to study motor and non-motor behaviors in adult zebrafish

Cited by 13 publications

References 47 publications

Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model: A 96-hour behavioral study

Characterization of neurobehavioral pattern in a zebrafish 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model: A 96-hour behavioral study

Cerebroventricular Microinjections of MPTP on Adult Zebrafish Induces Dopaminergic Neuronal Death, Mitochondrial Fragmentation, and Sensorimotor Impairments

MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline

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