A simple miniature gradient system for micro‐HPLC

Šlais, Karel; Preussler, V.

doi:10.1002/jhrc.1240100206

Cited by 10 publications

(2 citation statements)

References 14 publications

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“…This enables faster generation of pH gradient at initial stages of electrofocusing compared to IEF mode (where low-conductivity zones are often formed) and thus, a fast focusing. Based on a voltage drop applied, the separation power of ITP can be comparable to that of IEF in terms of resolution and limiting zone capacities under conditions where the gradient is smooth and the zones can be considered as Gaussian ones [7,8]. Indeed, ITP mode of electrofocusing was previously used in a continuous flow device [9,10] in addition to numerous examples in both gel and capillary formats.…”

Section: Generalmentioning

confidence: 99%

Electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis

Šlais

Št́astná

2014

Electrophoresis

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In this paper, we suggest new electrolyte system for fast preparative electrofocusing in wide pH range. It is based on bidirectional ITP with multiple counterions and spacers created by commercially available defined simple buffers. The migration course of proposed focusing model can be simulated in advance by using separation conditions and electrolyte components that are consequently applied during the experiments. The suggested electrolyte system allows high current densities at the initial stages of focusing without danger of local overheating, which strongly reduces the time needed for analysis completion. The performance of the electrolyte system is demonstrated by the focusing of synthetic colored low molecular weight indicators and proteins in the arrangements with both linear narrow strip and nonwoven fabric sheet with continuous flow.

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Section: Generalmentioning

confidence: 99%

Electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis

Šlais

Št́astná

2014

Electrophoresis

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“…An internal pH gradient of a wide range of pH can be developed using the pumped mobile phase containing only a few [3][4][5][6] simple buffering salts which differ in pka values by about 1 pk, unit or less. The use of several types of surface ionizable groups allows the internal pH gradient to be effective not only for separation of ampholytes but also for separation of anions of both strong and weak acids.…”

mentioning

confidence: 99%

Microcolumn liquid chromatography with sample induced internal pH gradient

Šlais

1991

J Microcolumn Separations

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Abstract. An internal pH gradient of a wide range of pH can be developed using the pumped mobile phase containing only a few [3][4][5][6] simple buffering salts which differ in pka values by about 1 pk, unit or less. The use of several types of surface ionizable groups allows the internal pH gradient to be effective not only for separation of ampholytes but also for separation of anions of both strong and weak acids. The internal pH gradient can be induced simultaneously with solute introduction into the separation column. For this new method, the term "sample induced internal gradient" (SIIG) of pH was suggested. The method is demonstrated by separation of model mixtures of UV detectable anions and ampholytes on a dynamically modified microcolumn.

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A step gradient system for microcolumn liquid chromatography based on a multiple‐loop valve

Banks

Novotný

1990

J Microcolumn Separations

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Abstract. A new gradient elution system for microcolumn liquid chromatography is presented and evaluated. A multiple-loop valve system facilitates a reproducible, stepwise addition of the mobile phase at low-microliter-per-minute flow rates. The system is amenable to automation. Gradient precision has been evaluated under various operational conditions. An optimized system yielded standard deviations in retention times of less than 0.5 on runs over 2 h.

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A simple miniature gradient system for micro‐HPLC

Abstract: SummaryA miniaturized continuous gradient elution system with variable gradient profile was designed. A retention time reproducibility below 1% was achieved. The system was applied to the separation of phenolic compounds with a wide range of polarities.

Cited by 10 publications

References 14 publications

Electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis

Electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis

Microcolumn liquid chromatography with sample induced internal pH gradient

A step gradient system for microcolumn liquid chromatography based on a multiple‐loop valve

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