A simple parallel gas chromatography column screening system

Abstract

A simple approach to the automated screening of four different columns on a single gas chromatography (GC) instrument is used for rapid chiral GC method development. Configuration of a conventional GC instrument with a second autosampler and several inexpensive Y-splitters enables simultaneous evaluation of two different columns, allowing a total of four different columns to be evaluated in two automated back to back runs. The resulting system affords a simple and effective approach to chiral GC method development that speeds analysis while eliminating the need for slow and tedious manual interchange of columns. An example of developing a rapid isothermal GC method from the screening results obtained by the instrument is also shown.

Chiral Separation of Diastereomeric and Enantiomeric Products Obtained by an Organic Reaction in Aqueous Media between Cyclohexanone and p‐nitrobenzaldehyde by HPLC on Chiral Stationary Phase

“…[ 4 ] Schafer and coworkers report an approach to the automated screening of four different columns on a single gas chromatography instrument for the rapid development of the chiral gas chromatography method. [ 5 ] In a paper by Hegade et al., [ 6 ] Chiral Stationary‐Phase Optimized Selectivity Liquid Chromatography is proposed as a tool to separate the mixtures of enantiomers on a series of coupled chiral columns available on the market. In this paper an analytical strategy, based on separation on chiral column, was applied to the organic reaction between cyclohexanone and benzaldehyde (variously substituted) in presence of a catalyst in heterogeneous phase and in water in different conditions of reaction.…”

Implementation of Hadamard encoding for rapid multisample analysis in liquid chromatography

“…Since conventional chromatographic techniques only allow sequential analysis of samples different strategies have been developed to increase sample throughput. This includes miniaturization to decrease run times and parallelization of chromatographic instruments, which has been implemented in both GC and HPLC . Welch et al.…”

“…Since conventional chromatographic techniques only allow sequential analysis of samples different strategies have been developed to increase sample throughput. This includes miniaturization to decrease run times [9][10][11][12] and parallelization of chromatographic instruments, which has been implemented in both GC and HPLC [13][14][15][16][17]. Welch et al developed multiple injections in a single experimental run chromatography, where in analogy to the FIA technique, rapid sequential analysis of samples is carried out in a single run using HPLC or HPLC-MS [18].…”