A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents

Kubota, Naoji; Tatsumoto, Nobuhide; Sano, Takayuki; Toya, Kaori

doi:10.1016/s0008-6215(99)00263-3

Cited by 253 publications

(155 citation statements)

References 15 publications

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“…Higher concentration of LMWC in comparison to TPP resulted in no change in LMWC solution as occurred with formulations LCN 1 , LCN 6 , LCN 7 , LCN 11 , LCN 12 , LCN 16 , LCN 17 , and LCN 18 . Similar concentration of Gal-LMWC(s) to TPP resulted in no change in Gal-LMWC(s) solution as occurred with formulations G 1 CN 6 , G 1 CN 11 , G 1 CN 12 , G 1 CN (Table I).…”

Section: Resultsmentioning

confidence: 89%

“…The pHdependent solubility of native chitosan and LMWC was evaluated by turbidity measurement technique (17), where the turbidity of polymer was measured at 600 nm on changing the pH of the solution. Infrared spectra of the native chitosan and LMWC were recorded on a Fourier transform infrared spectrophotometer using the potassium bromide (KBr) disk technique.…”

Section: Methodsmentioning

confidence: 99%

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Development and In Vitro Characterization of Galactosylated Low Molecular Weight Chitosan Nanoparticles Bearing Doxorubicin

Jain

2010

AAPS PharmSciTech

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Abstract. The aim of the present research was to evaluate the potential of galactosylated low molecular weight chitosan (Gal-LMWC) nanoparticles bearing positively charged anticancer, doxorubicin (DOX) for hepatocyte targeting. The chitosan from crab shell was depolymerized, and the lactobionic acid was coupled with LMWC using carbodiimide chemistry. The depolymerized and galactosylated polymers were characterized. Two types of Gal-LMWC(s) with variable degree of substitution were employed to prepare the nanoparticles using ionotropic gelation with pentasodium tripolyphosphate anions. Factors affecting nanoparticles formation were discussed. The nanoparticles were characterized by transmission electron microscopy and photon correlation spectroscopy and found to be spherical in the size range 106-320 nm. Relatively higher percent DOX entrapment was obtained for Gal-LMWC(s) nanoparticles than for LMWC nanoparticles. A further increase in drug entrapment was found with nanoparticles prepared by Gal-LMWC with higher degree of substitution. A hypothesis which correlates the ionic concentration of DOX in nanoparticles preparation medium and percent DOX entrapment in cationic polymer has been proposed to explain the enhanced DOX entrapment. In-vitro drug release study demonstrated an initial burst release followed by a sustained release. The targeting potential of the prepared nanoparticles was assessed by in vitro cytotoxicity study using the human hepatocellular carcinoma cell line (HepG 2 ) expressing the ASGP receptors on their surfaces. The enthusiastic results showed the feasibility of Gal-LMWC(s) to entrap the cationic DOX and targeting potential of developed Gal-LMWC(s) nanoparticles to HepG 2 cell line.

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Section: Resultsmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Development and In Vitro Characterization of Galactosylated Low Molecular Weight Chitosan Nanoparticles Bearing Doxorubicin

Jain

2010

AAPS PharmSciTech

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“…PVA is also a material easy to be electrospun and thus can be used as core material for coaxial electrospinning [11,12]. Chitosan is a natural basic polysaccharide composed of glucosamine and N-acetyl glucosamine [13]. It has been used in drug delivery, tissue engineering, and other biomedical applications because it is biocompatible, biodegradable and an-timicrobial.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and application of coaxial polyvinyl alcohol/chitosan nanofiber membranes

et al. 2017

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It is difficult to fabricate chitosan-wrapped coaxial nanofibers, because highly viscous chitosan solutions might hinder the manufacturing process. To overcome this difficulty, our newly developed method, which included the addition of a small amount of gum arabic, was utilized to prepare much less viscous chitosan solutions. In this way, coaxial polyvinyl alcohol (PVA)/chitosan (as core/shell) nanofiber membranes were fabricated successfully by coaxial electrospinning. The core/shell structures were confirmed by TEM, and the existence of PVA and chitosan was also verified using FT-IR and TGA. The tensile strength of the nanofiber membranes was increased from 0.6-0.7 MPa to 0.8-0.9 MPa after being crosslinked with glutaraldehyde. The application potential of the PVA/chitosan nanofiber membranes was tested in drug release experiments by loading the core (PVA) with theophylline as a model drug. The use of the coaxial PVA/chitosan nanofiber membranes in drug release extended the release time of theophylline from 5 minutes to 24 hours. Further, the release mechanisms could be described by the Korsmeyer-Peppas model. In summary, by combining the advantages of PVA and chitosan (good mechanical strength and good biocompatibility respectively), the coaxial PVA/chitosan nanofiber membranes are potential biomaterials for various biomedical applications.

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“…The amino groups (pKa from 6.2 to 7.0) are completely protonated in acids with pKa smaller than 6.2 making chitosan soluble. Chitosan is insoluble in water, organic solvents and aqueous bases and it is soluble after stirring in acids such as acetic, nitric, hydrochloric, perchloric and phosphoric (Guibal, 2004;Kluget al, 1998;Kubota et al, 2000;Kurita, 2006;Anthonsen & Smidsroed, 1995;Rinaudo, 2006;Sankararamakrishnan & Sanghi, 2006 Food contaminations and the negative environmental impact of packaging materials currently in use have prompted the research of safer materials. Chitosan have shown great promise to be used in food industry as preservative because of its high antimicrobial activity against various microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Properties of Chitosan

Alvarenga¹

2011

Biotechnology of Biopolymers

123

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A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents

Cited by 253 publications

References 15 publications

Development and In Vitro Characterization of Galactosylated Low Molecular Weight Chitosan Nanoparticles Bearing Doxorubicin

Development and In Vitro Characterization of Galactosylated Low Molecular Weight Chitosan Nanoparticles Bearing Doxorubicin

Fabrication and application of coaxial polyvinyl alcohol/chitosan nanofiber membranes

Characterization and Properties of Chitosan

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