A simple qualitative determination of human antibodies to murine immunoglobulins (HAMA) in serum samples

Seccamani, E.; Tattanelli, M; Mariani, Massimo A.; Spranzi, E; Scassellati, G. A.; Siccardi, A. G.

doi:10.1016/0883-2897(89)90191-8

Cited by 41 publications

(26 citation statements)

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“…No antibiotic medication was used to prevent allergic inflammation. Antibody: A mouse monoclonal antibody against TNC, clone 4F10TT, was raised by immunization of a TNC-null mouse [7][8][9] with purified human TNC 10) as described previously.…”

Section: Methodsmentioning

confidence: 99%

Noninvasive Detection of Cardiac Repair After Acute Myocardial Infarction in Rats by 111In Fab Fragment of Monoclonal Antibody Specific for Tenascin-C

et al. 2008

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SUMMARYLeft ventricular (LV) remodeling after acute myocardial infarction (MI) causes heart failure, and thus it is important to evaluate cardiac repair as the early stage of LV remodeling. Tenascin-C (TNC), an extracellular matrix glycoprotein, is transiently and abundantly expressed in the heart during the early stage of tissue remodeling after MI. However, it is not expressed in healthy adult heart. This study was undertaken to develop a new noninvasive diagnostic technique to detect cardiac repair after acute MI using 111 In Fab fragment of a monoclonal antibody specific for TNC.111 In-anti-TNC-Fab was injected intravenously in 13 rats at 1 (D1, n = 3), 3 (D3, n = 5), and 5 (D5, n = 5) days after producing MI and in 5 sham-operated rats (S). We performed autoradiography and dual-isotope single-photon emission computed tomography imaging (SPECT) of 111 In-anti-TNC-Fab and 99mTc methoxyisobutyl isonitrile (MIBI). The radioactivity in the heart was significantly higher in D (D1, 0.45 ± 0.06% injected-dose/g; D3, 0.64 ± 0.12; D5, 0.38 ± 0.07) than S (0.27 ± 0.06, P < 0.01 versus D1 and D3, P < 0.05 versus D5). By autoradiography, higher radioactivities were observed in the infarcted area than in the noninfarcted area of MI hearts. Dual-isotope SPECT demonstrated the regional myocardial uptake of 111 In-anti-TNC-Fab, which was complementary to the perfusion image.The results of the present study indicated that we can localize the infarcted region in the heart by ex vivo and in vivo imaging methods using 111 In-anti-TNC-Fab, and suggested the potential usefulness of noninvasive detection of cardiac repair. (Int Heart J 2008; From the

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Section: Methodsmentioning

confidence: 99%

Noninvasive Detection of Cardiac Repair After Acute Myocardial Infarction in Rats by 111In Fab Fragment of Monoclonal Antibody Specific for Tenascin-C

et al. 2008

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“…The induction of human anti-mouse immunoglobulin antibodies (HAMA) was verified using an ELI-SA system [14]. Avidin immunogenicity (human anti-avidin response, HAAR) was studied on microwell plates coated with avidin or streptavidin separately.…”

Section: Methodsmentioning

confidence: 99%

Pre-targeted immunodetection in glioma patients: tumour localization and single-photon emission tomography imaging of [99mTc]PnAO-biotin

et al. 1994

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The imaging of cerebral gliomas with radiolabelled monoclonal antibodies (MoAbs) has been previously reported. However, previous studies have been hampered by the drawback of a low tumour to non-tumour ratio. In order to overcome this problem we have developed a three-step pre-targeting method using the avidin-biotin system. The rationale of this technique consists in vivo labelling of biotinylated MoAbs targeted onto tumour deposits, when most of the unbound antibodies have been cleared from the bloodstream as avidin-bound complexes. The anti-tenascin MoAb BC2, specific for the majority of gliomas, was biotinylated and 1 mg was administered i.v. in 20 patients with histologically documented cerebral lesions. After 24-36 h, 5 mg avidin Was injected i.v. followed 24 h later by a third i.v. injection of 0.2 mg PnAO-biotin labelled with 15-20 mCi technetium-99m. No evidence of toxicity was observed. Whole-body biodistribution was measured at 20 min, 3 h and 5 h post-injection.[99mTc]PnAO-biotin had a fast blood clearance and was primarily excreted through the biliary system. A dedicated single-photon emission tomography system was used to acquire brain tomographic images 1-2 h after the administration of [99mTc]PnAO-biotin. Tumours were detected in 15/18 glioma patients with a tumour to non-tumour ratio of up 14:1. This three-step method, based on the sequential administration of anti-tenascin MoAb BC2, avidin and [99mTc]PnAO-biotin, can support computed tomography or magnetic resonance imaging for the diagnosis and follow-up of patients with glioma. Further studies are required to evaluate the potential of this technique for therapeutic application.

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“…A new strategy for designing haptens and generating abzymes with GPX activity was proposed (Su et al, 2001), and a series of mouse monoclonal antibodies and scFv were obtained and converted into catalytic antibodies with GPX activities by chemical modification (Luo et al, 1994;Ding et al, 1998;Ren et al, 2001;Su et al, 2001). Although most of them showed excellent enzymic activity and biological effect, mouse monoclonal antibodies or scFvs may cause potential immunoreaction due to the antigenicity to humans (Reynolds et al, 1989;Seccamani et al, 1989).…”

Section: Introductionmentioning

confidence: 98%

A new human catalytic antibody Se‐scFv‐2D8 and its selenium‐containing single domains with high GPX activity

Song

et al. 2010

J of Molecular Recognition

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Glutathione peroxidase (GPX) is a well-known antioxidant selenoenzyme, which can catalyze the reduction of a variety of hydroperoxides and consequently protect cells and other biological tissues against oxidative damage. Many attempts have been made to mimic its function, and a human catalytic antibody Se-scFv-B3 with GPX activity has been prepared in our previous study. This time, a new clone 2D8 that bound specifically to the glutathione analog GSH-S-DNPBu was selected again by using the technology of phage display antibody library, and then scFv-2D8 was successfully expressed in soluble form and purified using Ni(2+)-immobilized metal affinity chromatography. After being converted into selenium-containing scFv by chemically modification, it showed higher GPX activity than previous abzyme Se-scFv-B3. The heavy chain variable fragment of scFv-2D8 was also prepared and converted into selenium-containing protein using the same method. This selenium-containing single-domain antibody showed some GPX activity and, to the best of our knowledge, is the first human single-domain abzyme with GPX activity, which lays a foundation for preparing GPX abzyme with human origin, lower molecular weight and higher activity.

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A simple qualitative determination of human antibodies to murine immunoglobulins (HAMA) in serum samples

Cited by 41 publications

References 1 publication

Noninvasive Detection of Cardiac Repair After Acute Myocardial Infarction in Rats by 111In Fab Fragment of Monoclonal Antibody Specific for Tenascin-C

Noninvasive Detection of Cardiac Repair After Acute Myocardial Infarction in Rats by 111In Fab Fragment of Monoclonal Antibody Specific for Tenascin-C

Pre-targeted immunodetection in glioma patients: tumour localization and single-photon emission tomography imaging of [99mTc]PnAO-biotin

A new human catalytic antibody Se‐scFv‐2D8 and its selenium‐containing single domains with high GPX activity

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