A simple quantitative method to determine short chain fatty acid levels in biological fluids

Whitehead, James S.; Kim, Young S.; Prizont, Roberto

doi:10.1016/0009-8981(76)90193-5

Cited by 45 publications

(9 citation statements)

References 11 publications

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“…In the final set of experiments, [15][16][17][18][19][20] ,lCi of D-[U-'4C]-glucosamine hydrochloride were injected intraperitoneally into self-filling blind loop rats. Animals were killed and the blind loop contents were collected and processed as previously described.…”

Section: Methodsmentioning

confidence: 99%

Glycoprotein Degradation in the Blind Loop Syndrome

Prizont¹

1981

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

Glycoprotein Degradation in the Blind Loop Syndrome

Prizont¹

1981

J. Clin. Invest.

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“…Folate in whole blood was assayed by protein binding assay (26), and short-chain fatty acids (SCFA) in feces were assayed by gas-liquid chromatography (27).…”

Section: Biochemical Assaysmentioning

confidence: 99%

A Probiotic Strain of L. Acidophilus Reduces DMH-Induced Large Intestinal Tumors in Male Sprague-Dawley Rats

McIntosh¹,

Royle²,

Playne³

1999

Nutrition and Cancer

134

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Probiotic bacteria strains were examined for their influence on 1,2-dimethylhydrazine (DMH)-induced intestinal tumors in 100 male Sprague-Dawley rats. Lactobacillus acidophilus (Delvo Pro LA-1), Lactobacillus rhamnosus (GG), Bifidobacterium animalis (CSCC1941), and Streptococcus thermophilus (DD145) strains were examined for their influence when added as freeze-dried bacteria to an experimental diet based on a high-fat semipurified (AIN-93) rodent diet. Four bacterial treatments were compared: L. acidophilus, L. acidophilus + B. animalis, L. rhamnosus, and S. thermophilus, the bacteria being added daily at 1% freeze-dried weight (10(10) colony-forming units/g) to the diet. Trends were observed in the incidence of rats with large intestinal tumors for three treatments: 25% lower than control for L. acidophilus, 20% lower for L. acidophilus + B. animalis and L. rhamnosus treatments, and 10% lower for S. thermophilus. Large intestinal tumor burden was significantly lower for treated rats with L. acidophilus than for the control group (10 and 3 tumors/treatment group, respectively, p = 0.05). Large intestinal tumor mass index was also lower for the L. acidophilus treatment than for control (1.70 and 0.10, respectively, p < 0.05). Other treatments showed no statistically significant change from control for these indexes of tumorigenesis. For rats fed L. acidophilus, no adenocarcinomas were present in the colons. Pulsed-field gel electrophoresis of bacterial chromosomal DNA fragments was used to differentiate introduced (exogenous) bacterial strains from indigenous bacteria of the same genera present in the feces. Survival during gut passage and displacement of indigenous lactobacilli occurred with introduced L. acidophilus and L. rhamnosus GG during the probiotic treatment period. However, introduced strains of B. animalis and S. thermophilus were not able to be isolated from feces. It is concluded that this strain of L. acidophilus supplied as freeze-dried bacteria in the diet was protective, as seen by a small but significant inhibition of tumors within the rat colon.

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“…This was done by a modification of the method of Whitehead et al 25 The stored samples were thawed, mixed with a vortex mixer, and duplicate samples of the suspension were taken from the caecal contents (approximately 1 g). For samples from the mid‐colon and rectum, duplicate samples were taken only when the fresh weight of the sample was 1 g or more, otherwise a single sample was taken (approximately 2 g).…”

Section: Methodsmentioning

confidence: 99%