A Simple Raped Flocculation Slide Test for Trichinosis in Man and in Swine

Evaluation of the Suessenguth-Kline Test for Trichinosis

“…It had been found in an earlier study (6) that an alkaline aqueous extract of freeze-dried trichinae larvae possesses the property of coating cholesterol crystals so that, when such coated crystals are dispersed in physiological saline, an antigen emulsion is formed. When used in the slide test, the antigen emulsion is sensitive and specific for trichinosis.…”

ANALYSIS OF HELMINTH ANTIGENS (ECHINOCOCCUS GRANULOSUS AND SCHISTOSOMA MANSONI) BY AGAR GEL METHODS

“…The most commonly employed antigens in the bentonite flocculation test for trichinosis are an acid-soluble protein antigen prepared by the method of Melcher (1943), and a metabolic or excretion and secretion antigen of trichinella larvae (Norman and Sadun, 1959). In the cholesterol-lecithin flocculation test an alkaline extract of larvae is used (Suessenguth and Kline, 1944). In the complement fixation test, a heated saline extract has been used (Witebsky, Wels, and Heide, 1942).…”

Trichinosis in the United States

“…Two flocculation serologic procedures are available for the diagnosis of trichinosis: the method of Sussenguth and Kline (19), utilizing cholesterol particles, and the bentonite tech¬ nique of Bozicevich and others (20). The Helminthology Unit at the Communicable Disease Center uses the bentonite flocculation test for the diagnosis of trichinosis.…”

“…As a method of diagnosing active infection, the bentonite technique is excellent, but, for the de¬ tection of chronic infections, it does not com¬ pare with the technique of Sussenguth and Kline. These workers reported that anti¬ body may be detected in infected hogs 1 year after infection (19). Utilization of a rapid serologic technique for the detection of infected animals prior to slaughter might under some circumstances be a useful control procedure.…”