A simple, rapid, and quantifiable system for studying adventitious root formation in grapevine

Chang, Xin Yu; Zhang, Kai; Yuan, Yunzhang; Ni, Pei-Yi; Ma, Jing; Liu, Hui; Shouliang, Gong; Yang, Guo shun; Miao, Bai

doi:10.1007/s10725-022-00838-5

Cited by 11 publications

(6 citation statements)

References 57 publications

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“…The onset of AR was always delayed by NPA, regardless of the type of treatment. A similar response has been observed in other species such as pine, oak and grapevine [ 32 , 47 , 48 ]. In addition, auxin-induced activation of target-competent cells was not greatly impaired by the temporal (5 day) post-application of NPA, as once NPA was removed from the medium the cells were able to resume division and proceed to the rooting pathway.…”

Section: Discussionsupporting

confidence: 84%

“…The function of auxin signaling and transport in adventitious root formation have been reported in different species [ 2 , 52 , 53 , 54 ]. Inhibitors of plant auxin transport, such as NPA, were shown to delay and inhibit adventitious root formation [ 32 , 48 , 49 , 55 , 56 , 57 ]. A direct correlation between inhibition of AR by NPA and suppression of the 24 h peak of IAA during induction of adventitious roots was also demonstrated in petunia [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

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Expression of CsSCL1 and Rooting Response in Chestnut Leaves Are Dependent on the Auxin Polar Transport and the Ontogenetic Origin of the Tissues

Varas

Valladares

Vielba

et al. 2023

Plants

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The mechanisms underlying the de novo regeneration of adventitious roots are still poorly understood, particularly in trees. We developed a system for studying adventitious rooting (AR) at physiological and molecular levels using leaves excised from chestnut microshoots of the same genotype but with two distinct ontogenetic origins that differ in rooting competence. Leaves were treated with auxin and N-1-naphthyl-phthalamic acid (NPA), an inhibitor of auxin polar transport (PAT). The physiological effects were investigated by recording rooting rates and the number and quality of the roots. Molecular responses were examined by localizing and monitoring the changes in the expression of CsSCL1, an auxin-inducible gene in juvenile and mature shoots during AR. The rooting response of leaves was ontogenetic-stage dependent and similar to that of the donor microshoots. Initiation of root primordia and root development were inhibited by application of NPA, although its effect depended on the timing of application. CsSCL1 was upregulated by auxin only in rooting-competent leaves during the novo root organogenesis, and the expression was reduced by NPA. The inhibitory effect on gene expression was detected during the reprograming of rooting competent cells towards root initials in response to auxin, indicating that PAT-mediated upregulation of CsSCL1 is required in the initial steps of AR in chestnut leaves. The localized expression of CsSCL1 in the quiescent center (QC) also suggests a role for this gene in the maintenance of meristematic competence and root radial patterning.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Expression of CsSCL1 and Rooting Response in Chestnut Leaves Are Dependent on the Auxin Polar Transport and the Ontogenetic Origin of the Tissues

Varas

Valladares

Vielba

et al. 2023

Plants

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“…The results in Table 2 show that the NAA soaked concentration at various concentrations (S0, S1, S2 and S3) had no significant effect on the time of emergence of leaf on grape cuttings seedlings. The leaf emergence is influenced by the interaction between auxins and cytokinins found in cuttings (Salem et al, 2022); (Chang et al, 2022). In this study, as shown in Figure 2 it was suspected that the new root system was formed in the 2nd week so endogenous auxin had not been produced because the absorption of NAA soaked concentration was used by cuttings for initiation of root formation.…”

Section: Leaf Emergence Timementioning

confidence: 76%

An Utilizing Marginal Red Yellow Podzolic Soil as a Growing Medium for Auxin-Soaked Cutting Propagation of Red Master Grapes (Vitis vinifera L)

Pamungkas,

Pramudya,

Sukmawati

et al. 2023

J. appl. agricultural sci. technol.

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Grape (Vitis vinifera) cultivation is hampered by a lack of suitable land and challenging nurseries. Use of crimson yellow podzolic soil for vine cuttings can be suboptimal. Although podzolic soil has a low capacity to absorb macro- and micronutrients and an acidic pH, it can nevertheless be used as a growing medium. A synthetic growth regulator (ZPT) bath of naphthalene acetic acid (NAA) needs to be added to the local red master grape variety, which has started to develop as a scion in nurseries. In order to assess the impacts of NAA-soaked concentrations on podzolic soil media and identify the morphological traits of local Red Master grape cuttings, this study set out to identify these traits. Its goal was to find out how auxin affected the development and yield of grapes (Vitis vinifera L.) grown in red yellow podzolic soil. A non-factorial totally randomized design with one factor (NAA concentration) was used in the study, with a block of 4 treatments and 3 repetitions. The varied NAA concentrations used in the treatment procedure included F0 (0 g L-1), F1 (2 g L-1), F2 (4 g L-1), and F3 (6 g L-1). The variables that were observed included the number of shoot bursts, the timing of leaf emergence, the number of sheet leaves, the length of the tendrils, and the proportion of live cuttings. ANOVA was used to tabulate and analyze observational data at a 5% level, and DMRT analysis was used to continue the analysis at the same level. According to the results, soaked NAA at a concentration of 0 g L-1 (S0) as the control treatment had the best results for the variable number of shoots and leaves, while a concentration of 2 g L-1 (S1) had the best results for the variable length of tendrils. However, soaked NAA had no significant impact on the variable when leaves emerged. Soaking auxin at the bottom of cuttings had no effect on shoot formation. Reduction of auxin due to defoliation can result in the expression of the isopentenyl-transferase (IPT) gene. Auxins, such as Indole-3-acetic acid (NAA), are usually involved in the regulation of root and leaf growth. If a plant has many leaves and few roots without the use of additional NAA or auxin, several factors may play a role, such as plant genetics, environmental conditions, and environmental stress. The use of additional NAA or auxin can explicitly affect the growth of roots and leaves. The survival percentage of cuttings is still low because it is influenced by the quality (material) of the cuttings, the age of the parent tree, growing media and water availability.

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“…In this study, the rejuvenated and mature cuttings both displayed a decrease in tZR levels, but this decrease was more pronounced at 14 days in the rejuvenated shoots, which was in agreement with the general observation that cytokinin depletion participates in the induction of adventitious roots in cuttings [ 38 ]. GAs have been shown to significantly inhibit ARF and development [ 40 ]. External application of GAs has been shown to inhibit rooting, possibly by inhibiting auxin polar transport [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Grafting Causes Physiological Changes and Promotes Adventitious Root Formation in Rejuvenated Soft Shoots of Taxodium hybrid ‘Zhongshanshan’

Wang

Shi

Chen³

et al. 2023

Plants

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Taxodium hybrid ‘Zhongshanshan’ has been widely used as a timber tree in river network areas and coastal regions and is mainly propagated by cuttings. However, when trees age, their capacity to form adventitious roots becomes weaker. We successfully enhanced the rooting ability of shoots in T. hybrid ‘Zhongshanshan 302’ by their rejuvenation based on grafting. We recorded temporal variation in endogenous auxin, abscisic acid (ABA), gibberellins (GAs), trans-zeatin-riboside (TZR), soluble sugar and H2O2 after root induction. Auxin, soluble sugars and H2O2 levels were higher in rejuvenated shoots than in mature shoots, whereas the opposite was true for ABA and GAs. Notably, indole-3-acetic acid (IAA) and GA3 presented higher contents with more obvious differences in T. hybrid ‘Zhongshanshan 302’ rejuvenated shoots vs. mature shoots compared with other kinds of auxin and GAs. The evident improvement in the rooting ability of rejuvenated shoots after grafting likely resulted from the differential regulation of plant hormones, carbohydrates and redox signaling. In addition to the physiological basis of improved rooting ability by grafting, this study provided a theoretical basis for the optimization of subsequent propagation techniques in T. hybrid ‘Zhongshanshan’ and potentially other Taxodium spp.

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A simple, rapid, and quantifiable system for studying adventitious root formation in grapevine

Cited by 11 publications

References 57 publications

Expression of CsSCL1 and Rooting Response in Chestnut Leaves Are Dependent on the Auxin Polar Transport and the Ontogenetic Origin of the Tissues

Expression of CsSCL1 and Rooting Response in Chestnut Leaves Are Dependent on the Auxin Polar Transport and the Ontogenetic Origin of the Tissues

An Utilizing Marginal Red Yellow Podzolic Soil as a Growing Medium for Auxin-Soaked Cutting Propagation of Red Master Grapes (Vitis vinifera L)

Grafting Causes Physiological Changes and Promotes Adventitious Root Formation in Rejuvenated Soft Shoots of Taxodium hybrid ‘Zhongshanshan’

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