A simple robust method of synthesis of copper–silver core–shell nano-particle: evaluation of its structural and chemical properties with anticancer potency

Abstract

A simple method of synthesis of a stable bimetallic copper-silver nano-particle (CuAg-NP) was developed by successive reduction of Cu(NO) and AgNO, using hydrazine hydrate as the reducing agent and gelatin and poly-vinyl pyrrolidone (PVP) as the capping agents. The round-shaped particles were of a core-shell structure with a core of Cu atoms surrounded by a shell of Ag atoms. The size and the mol. wt. of the NPs were (100 ± 10) nm and (820 ± 157) Kd, respectively; the particles were crystalline in nature and 90% of the precursors Cu(NO) and AgNO were converted to the NPs. The particles were more toxic to cancer cells than normal cells; the dose of the NPs (4-5 μg ml), that killed about 75% of the different human cancer cell lines viz, HepG2 (liver cancer), A549 (lung cancer) and AGS (stomach cancer), killed only about 22.5% of the normal cell lines viz, WRL68 (liver) and WI38 (lung). Therefore, the NP may be developed as a potent anticancer drug in future. The more detailed study on the cytotoxicity of the CuAg-NP on the HepG2 cell line revealed that the particles caused cell cycle arrest in a G2/M phase, depolarization of mitochondrial membrane potential, translocation of phosphatidylserine residues from inner to outer leaflets of cell membrane and DNA degradation; these phenomena confirmed that the NP-induced cell death was apoptotic in nature.

Facile Synthesis, Characterization, Anti-Microbial and Anti-Oxidant Properties of Alkylamine Functionalized Dumb-Bell Shaped Copper-Silver Nanostructures

“…The blue shift in absorbance spectra is expected to the Cu NPs and redshift is expected to the Ag NPs. We found that the obtained results are in good agreement with the previous reports for Cu: Ag ratio [28][29][30]. The location of the precise metal NPs plasmon absorption band determines the shape and size of the NPs.…”

A novel CuFe2O4@Ag nanocomposite biosynthesized bySpirulina platensisexhibits an anticancer effect on human gastric adenocarcinoma and Michigan Cancer Foundation‐7 breast cancer cell lines

“…This early apoptotic cell population was greater than those reported for cancer cell lines treated with the Fe 2 O 4 @Ag nanocomposite (13.2%) [ 30 ] and Cu–Ag NPs (24.53%). [ 31 ] These results confirmed the changes in the mitochondrial membrane, which ultimately led to a loss of the membrane potential function. The late apoptosis percentage exposed by CuFe 2 O 4 @Ag NPs (Q 2 ) was 5.57% compared to 6.49% for the control cells.…”

Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout

“…The vibration bands at 3444 and 3387 cm −1 could be attributed to primary amines (NH 2 ) [34]; the carboxylic acid (OH) stretch was located at 2936 cm −1 [35]. However, peaks at 2356 cm −1 could be assigned to C-H asymmetric stretching vibration for aliphatic groups [36]. Meanwhile, the spectral peaks in around 1644, 1636 and 1744 cm −1 implied the presence of -C=C bond [36].…”