A simple, sensitive and efficient assay for the determination of d- and l-lactic acid enantiomers in human plasma by high-performance liquid chromatography

Establishment of a two-dimensional chiral HPLC system for the simultaneous detection of lactate and 3-hydroxybutyrate enantiomers in human clinical samples

“…The LOD of LA was 1/40 of that determined by the previously used column-switching HPLC method [28], which had an LOD of 400 fmol/injection. The study conducted by Cevasco et al established a quick and easy method to analyze and separate the enantiomers of LA by using HPLC, but the LOD was 5 µΜ for D-LA suggesting that D-LA might not be detected at lower concentrations [29]. However, in our study, the LOD values of the LA enantiomers determined by the 2D-HPLC method were approximately 1/40 or lower than those obtained by the previous methods.…”

L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry

“…So far, D- and L-Lactate have been analysed by several different techniques ranging between chiral stationary phase liquid chromatography using UV or fluorescence detection [3, 11–15], enzymatic assays [7, 16–21], gas chromatography mass spectrometry (GC/MS) methods [22, 23], liquid chromatography mass spectrometry (LC/MS) methods [24, 25], and reversed phase liquid chromatography using fluorescence detection [26]. However, these techniques have several shortcomings such as low sensitivity [11, 24, 27], large sample volume [19, 21, 22], complex chromatographic systems [3, 12, 13], and long run times [3, 11, 26, 27].…”

“…However, these techniques have several shortcomings such as low sensitivity [11, 24, 27], large sample volume [19, 21, 22], complex chromatographic systems [3, 12, 13], and long run times [3, 11, 26, 27]. To further explore the use of D-lactate as a biomarker, there is a need of an improved method for analysing D-lactate.…”

The analysis of advanced glycation endproducts

“…To investigate the glyoxalase pathway, in particular the detoxification of this MGO into D-lactate, there is need of a sensitive and highly specific method to determine D-lactate. Several different techniques have been described with some shortcomings such as low sensitivity [56][57][58] , large sample volume [59][60][61] , complex chromatographic systems [62][63][64] and long run times 56,58,62,65 . In chapter 4 a highly sensitive, specific and fast UPLC-MS/MS method to determine L-and D-lactate in plasma and urine is described.…”

“…For the first time AGE accumulation was estimated in long-lived proteins, such as collagen and lens crystallins, and this was closely related to the pathogenesis of aging and diabetes 17,30,62 . More recent, skin autofluorescence (SAF) 63 is used as a non-invasive tool to assess the AGE accumulation in the skin and can be useful for rapid risk assessment of AGE-related long-term complications in diabetes and related diseases [64][65][66][67] . SAF illuminates approximately 1 cm 2 skin with an excitation light source of 300 to 420 nm.…”