A simple “signal off–on” fluorescence nanoplatform for the label-free quantification of exosome-derived microRNA-21 in lung cancer plasma

Wei, Jinlan; He, Sitian; Mao, Yanhua; Wu, Longjie; Liu, Xinlian; Effah, Clement Yaw; Guo, Hongchao; Wu, Yongjun

doi:10.1007/s00604-021-05051-1

Cited by 11 publications

(3 citation statements)

References 37 publications

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“…21 FRET sensors coupled with MoS 2 nanosheets have been combined with hybridization chain reaction amplification to detect exosomal miRNA-21 in the plasma of patients with lung cancer (LOD: 6 pM). 22 Hierarchical MoS 2 nanostructuring and silicon dioxide nanosignal amplification-based FRET nanoprobes were combined with catalytic hairpin assembly (CHA) technology for miRNA detection in circulating tumor cells (CTCs) with an LOD of 300 pM, which demonstrated the advances in FRET nanosensors for in situ miRNA detection in liquid biopsy 23 (Fig. 1c).…”

Section: Optical Nanobiosensors Applied In Liquid Biopsymentioning

confidence: 99%

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

Ya,

Zhang,

Wang

et al. 2024

Nanoscale

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Section: Optical Nanobiosensors Applied In Liquid Biopsymentioning

confidence: 99%

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

Ya,

Zhang,

Wang

et al. 2024

Nanoscale

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“…It has an outstanding fluorescence-quenching feature and the absorbability of single-stranded nucleic acid [9] [10]. At present, a large number of studies have applied this feature to the detection of small molecules such as miRNA [11] and DNA [12] as well as the biological analysis of large molecules such as carcinoembryonic antigen, prostate specific antigen [13], and cardiac troponin T [14]. Such applications are conducive to the development of basic research in life science and the reform of medical clinical detection technology.…”

Section: Introductionmentioning

confidence: 99%

Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB<sub>1</sub>

Wen¹,

Song²,

Cui³

et al. 2023

JBM

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Purpose: Aflatoxin B 1 is the most common mycotoxin in cereal crops; it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS 2 NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB 1 by utilizing MoS 2 NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS 2 NS applied for the detection of AFB 1 . The fluorescence of Apt@AFB 1 can be quickly quenched by MoS 2 NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB 1 exists, after the specificity binding between AFB 1 and aptamer, the Apt@AFB 1 loses its single stranded structure and is away from MoS 2 NS, and the fluorescence of Apt@AFB 1 cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB 1 . Result: With this new method, under the optimized conditions, the AFB 1 is analyzed in the MoS 2 NS/Apt@AFB 1 sensing platform. Within the dynamic range of 0.2 -25 ng/mL, the sensing platform expresses a good linear response to the level of AFB 1 with the R 2 = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% -107.23% and 95.15% -102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB 1 . This detection method features several advantages such as specificity, rapidness and low costs, which can meet the requirement of trace detection in clinical detection and food safety.

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“…For example, breast tumor has at least three subtypes classified according to the tumor cell molecular profiles. The surface proteins of exosomes are specific and can reflect the physiological information on the parental cells. , A variety of exosomal proteins have been used to identify the tumor origins or subtypes, as well as distinguish between indolent and high-risk lesions. − However, highly accurate technologies for profiling and classifying the phenotype of cancers based on exosomal-specific proteins are still lacking.…”

mentioning

confidence: 99%

Highly Accurate Profiling of Exosome Phenotypes Using Super-resolution Tricolor Fluorescence Co-localization

Wei,

Zhu,

Wang

et al. 2024

ACS Nano

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Exosomes contain a wealth of proteomic information, presenting promising biomarkers for the noninvasive early diagnosis of diseases, especially cancer. However, it remains a great challenge to accurately and reliably distinguish exosomes secreted from different types of cell lines. Fluorescence immunoassay is frequently used for exosome detection. Nonspecific adsorption in immunoassays is unavoidable and affects the reliability of assay results. Despite the fact that various methods have been proposed to reduce nonspecific adsorption, a more effective method that can eliminate the influence of nonspecific adsorption is still lacking. Here, we report a more convenient way (named SR-TFC) to remove the artifacts caused by nonspecific adsorption, which combines tricolor fluorescence labeling of target exosomes, tricolor super-resolution imaging, and pixel counting. The pixel counting method (named CFPP) is realized by MATLAB and can eliminate nonspecific binding sites at the single-pixel level, which has never been achieved before and could improve the reliability of detection to the maximum extent. Furthermore, as a proof-of-concept, profiling of exosomal membrane proteins and identification of breast cancer subpopulations are demonstrated. To enable multiplex breast cancer phenotypic analysis, three kinds of specific proteins are labeled to obtain the 3D phenotypic information on various exosomes. Breast cancer subtypes can be accurately identified according to the super-resolution images of some clinically relevant exosomal proteins. Worth mentioning is that, by selecting other biomarkers, classification of other cancers could also be realized using SR-TFC. Hence, the present work holds great potential in clinical cancer diagnosis and precision medicine.

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A simple “signal off–on” fluorescence nanoplatform for the label-free quantification of exosome-derived microRNA-21 in lung cancer plasma

Cited by 11 publications

References 37 publications

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB<sub>1</sub>

Highly Accurate Profiling of Exosome Phenotypes Using Super-resolution Tricolor Fluorescence Co-localization

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A simple “signal off–on” fluorescence nanoplatform for the label-free quantification of exosome-derived microRNA-21 in lung cancer plasma

Cited by 11 publications

References 37 publications

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB&lt;sub&gt;1&lt;/sub&gt;

Highly Accurate Profiling of Exosome Phenotypes Using Super-resolution Tricolor Fluorescence Co-localization

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Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB<sub>1</sub>