A Simple Sperm DNA Toroid Integrity Test and Risk of Miscarriage

Chan, Philip J.; Orzylowska, E.M.; Corselli, J.; Jacobson, John D.; Wei, Albert K

doi:10.1155/2015/780983

Cited by 5 publications

(1 citation statement)

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“…The ability of these staining methods to identify alterations in chromatin status is demonstrated by higher percentages of stained mouse sperm under different conditions, such as treatment with estrogens (30%) [46], oxidative stress, age (30-70%) [47], and protamine 1 (90%) or protamine 2 deficiencies (30%) [48]. In comparison to other species, alterations in chromatin status in the mouse are rather low; studies of human, bull, boar, dog, and cat sperm revealed higher proportions of spermatozoa with defects in sperm chromatin compaction [39,42,55,[62][63][64][65].…”

Section: Discussionmentioning

confidence: 99%

Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems

Agudo-Rios,

Sanchez-Rodriguez,

Idrovo

et al. 2023

IJMS

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Sperm DNA integrity and chromatin status serve as pivotal indicators of sperm quality, given their intricate link to sperm function, embryo development, and overall fertility. Defects in chromatin compaction, which are often associated with compromised protamine content, can lead to damaged DNA strands. In this study, the chromatin status and possible correlation with DNA damage was assessed in males of three mouse species: Mus musculus, M. spretus, and M. spicilegus. We employed various staining methods, including aniline blue, methylene blue (Diff-Quik), toluidine blue, and chromomycin A3, to assess chromatin compaction in cauda epididymal sperm. Samples were also analyzed by the sperm chromatin structure assay (SCSA) to estimate DNA fragmentation (%tDFI, %HDS). Analyses were carried out on freshly collected sperm and cells incubated for 3 h in a HEPES-buffered modified Tyrode’s medium simulating conditions of the female reproductive tract. Notably, the analysis of chromatin status yielded minimal abnormal values across all three species employing diverse methodologies. SCSA analyses revealed distinct variations in %tDFI between species. Following sperm incubation, the percentages of sperm stained with methylene blue exhibited differences among the species and were significantly correlated to the DNA fragmentation index. HDS demonstrated correlations with the percentages of sperm stained by aniline blue, methylene blue, and chromomycin A3. Overall, chromatin compaction was high across all species, with limited differences among them. The relationship between chromatin status and DNA integrity appeared to be related to levels of sperm competition among species.

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Section: Discussionmentioning

confidence: 99%

Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems

Agudo-Rios,

Sanchez-Rodriguez,

Idrovo

et al. 2023

IJMS

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The effect of human sperm chromatin maturity on ICSI outcomes

et al. 2018

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Because sperm chromatin may play a key role in reproductive success, we verify the associations between sperm chromatin abnormalities, embryo development and the ability to achieve pregnancy. The evaluation of sperm chromatin maturity using aniline blue (AB), chromomycin A3 (CMA3) and toluidine blue (TB) staining were carried out in group of males from infertile couples that underwent ICSI. Low levels of sperm chromatin abnormalities (< 16%) were found in most subjects (> 50%). A higher percentage of TB-positive sperm cells were discovered in the men from couples who achieved ≤ 50% fertilized oocytes compared to men who achieved > 50%. No significant differences were discovered by the applied tests between the men from couples who achieved ≤ 50% and those who achieved > 50% high-quality embryos on the 3rd or 5th day after fertilization, nor between the men from couples who achieved pregnancy and those who failed. The sperm chromatin maturity did not correlate with the ICSI results. However, the ROC analysis revealed a significant predictive value of TB-positive spermatozoa only for fertilization. Therefore, the TB assay can be considered as a useful test for the prediction of fertilization. Our findings suggest that the level of sperm chromatin abnormalities of the examined men was not clinically significant. No found associations between sperm chromatin maturity and embryo development and the ability to achieve pregnancy. We could not exclude the effects of the repairing processes in the fertilized oocyte. The use of complementary tests that verify the status of the sperm chromatin seems justified.

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