A simple staining method for quantifying the acrosomal status of cat spermatozoa

Pope, C. E.; Zhang, Y.Z.; Dresser, B. L.

doi:10.1016/0093-691x(91)90233-4

Cited by 69 publications

(78 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The staining methods of Williams and Fast Panótico 4 ® used for the morphological evaluation were efficient; however, the first stained the cells more clearly, improving the identification of morphological abnormalities. The Fast Green / Rose Bengal staining (Pope, Zhang, Dress, 1991), used with fresh semen, clearly highlighted rhea sperm acrosome in a dark violet color. Some problems were found when using vacuum blood collection tubes (Vacutainer ® Systems).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Reproductive characteristics of captive greater rhea (Rhea americana) males reared in the state of São Paulo, Brazil

Góes

Cavalcante

Nichi

et al. 2010

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

Rheas (Rhea americana) belongs to the ratite group. Considering the commercial significance of this birds, some techniques, such as semen collection, were standardized. In this study, 107 male rheas (3 to 4 years of age) reared in commercial farms in the state of São Paulo, Brazil, were used. Semen was collected during the breeding and offbreeding seasons of 2001, 2002, and 2003. Bird hierarchical behavior was observed. Birds were restrained performed using a box and a black hood. Semen was collected by digital pressure on the base of the phallus, which size was measured, and the presence or absence of spiral shape was observed. Immediately after collection, semen samples were evaluated for volume, motility, sperm concentration, and morphology. In a limited number of birds, blood samples were collected to measure testosterone levels. Among the 69 birds studied during the breeding season, 44 presented large phalluses, out of which 26 showed spiral shape. The method of semen collection was efficient. The following semen parameter results were obtained: volume (0.68 ±0.14 ml), motility (61.11±11.54%), sperm concentration (3.29±1.33 x10 9 sptz/ml), and number of spermatozoa per ejaculate (2.40±1.38x10 9 sptz/ml). Morphological abnormalities were analyzed and recorded. Testosterone levels were statistically different (p = 0.0161) between the breeding and non-breeding season (53.28±18.41 ng/ml and 5:57±3.81 ng/ml, respectively). Variations in phallus size were also found between the breeding and non-breeding seasons. Larger phalluses and higher testosterone levels were correlated with dominant behavior. The results of the present experiment confirmed that it is possible to collect semen from rheas, allowing the future use of biotechnologies such as artificial insemination.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Semen aliquots were fixed in formalin buffer solution for later analysis of sperm concentration and morphology. Acrosomal morphology was evaluated by mixing 10 L fresh semen and 10 L Fast Green / Rose Bengal dye (Pope;Zhang;Dress, 1991), and smearing the solution on a glass slide.…”

Section: Methodsmentioning

confidence: 99%

Reproductive characteristics of captive greater rhea (Rhea americana) males reared in the state of São Paulo, Brazil

Góes

Cavalcante

Nichi

et al. 2010

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

show abstract

“…Morphology evaluation was performed using fast green FCF-rose Bengal stain (Pope et al 1991). Each slide was examined at 1000!…”

Section: Semen Evaluationmentioning

confidence: 99%

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

et al. 2012

View full text Add to dashboard Cite

This study was designed for the identification of different sperm kinetic subpopulations in feline semen using artificial neural networks (ANNs) and for the evaluation of the effect of ejaculation on motility patterns of these subpopulations. Seven tomcats presented for routine orchiectomy were electroejaculated, and after 5 days, orchiectomized and epididymal tail sperms were collected. Sperm motility characteristics were evaluated using a computer-assisted sperm analyzer that provided individual kinetic characteristics of each spermatozoon. A total of 23 400 spermatozoa for electroejaculated and 9200 for epididymal tail samples were evaluated using a multivariate approach, comprising principal component analysis and ANN classification. The multivariate approach allowed the identification and characterization of three different and well-defined sperm subpopulations. There were significant differences before (epididymal tail spermatozoa) and after (electroejaculated sperm) ejaculation in sperm kinetic subpopulation characteristics. In both epididymal and ejaculated samples, the majority of subpopulation was characterized by high velocity and progressiveness; however, the electroejaculated samples showed significantly higher values, suggesting that the microenvironment of the epididymal tail could affect the sperm motility or, alternatively, seminal plasma could increase the kinetic characteristics of the spermatozoa, indicating that only after ejaculation, the spermatozoa express their motility potential. Nevertheless, further studies are required to clarify the functional significance of each kinetic subpopulation.

show abstract

“…As avaliações de integridade de membrana plasmática (HOST) foram realizadas segundo Bittencourt et al (11) , enquanto que as de integridade acrossomal foram feitas segundo Pope et al (12) e o teste de termorresistência lento (TTR) foi realizado segundo o CBRA (9) .…”

Section: Methodsunclassified

Ácido Ascórbico No Diluidor Para Criopreservação De Sêmen Caprino

et al. 2017

View full text Add to dashboard Cite

ResumoObjetivou-se determinar o efeito e o melhor nível de inclusão de ácido ascórbico em meio diluidor TRIS-Gema para criopreservação de sêmen caprino. Foram utilizados cinco ejaculados de três bodes da raça Anglo Nubiana. Cada ejaculado foi dividido em quatro alíquotas de 200 µL, compondo quatro tratamentos: um sem adição de ácido ascórbico (controle) e os demais com 0,0528; 0,1056 e 0,1584mg/mL de ácido ascórbico no diluidor TRIS-Gema. Avaliaram-se motilidade espermática progressiva e vigor espermático pós-diluição, pós-resfriamento e pós-descongelamento; integridade de membrana (HOST); integridade acrossomal e teste de termorresistência lento (TTR). Os dados foram submetidos à Análise de Regressão a 5% de probabilidade. Não houve diferença para motilidade progressiva e para vigor espermático, repectivamente, na pós-diluição; pós-resfriamento e pós-descongelamento, assim como para os testes complementares HOST e após 120 minutos do TTR, para motilidade e vigor, no pós-descongelamento (P>0,05). Houve comportamento quadrático com a inclusão do ácido ascórbico para integridade acrossomal (61,58%), com nível ótimo de 0,1006mg/mL de ácido ascórbico (P<0,05). A inclusão de 0,1006mg/mL de ácido ascórbico no diluidor TRIS-Gema melhorou a integridade acrossomal durante o processo de criopreservação, podendo ser uma alternativa na composição dos diluidores seminais caprinos. Palavras-chave: antioxidantes; espermatozoide; estresse oxidativo; TRIS-gema. AbstractThe objective of this study was to determine the optimal inclusion level of ascorbic acid in TRISyolk extenders for cryopreservation of goat semen. We used five ejaculates of three Anglo Nubian goats. Semen from each animal was divided into four 200μL aliquots, composing four treatments: no

show abstract

A simple staining method for quantifying the acrosomal status of cat spermatozoa

Cited by 69 publications

References 0 publications

Reproductive characteristics of captive greater rhea (Rhea americana) males reared in the state of São Paulo, Brazil

Reproductive characteristics of captive greater rhea (Rhea americana) males reared in the state of São Paulo, Brazil

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

Ácido Ascórbico No Diluidor Para Criopreservação De Sêmen Caprino

Contact Info

Product

Resources

About