A simple tandem mass spectrometry method for structural identification of pentose oligosaccharides

Tsai, Shang‐Ting; Hsu, Hsu-Chen; Ni, Chi-Kung

doi:10.1039/d3an00068k

Cited by 3 publications

(2 citation statements)

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“…Mass spectrometry (MS) is an attractive possibility for carbohydrate detection, especially because MS is a fast and high-throughput analysis method 53,54 . Part of the difficulty in monosaccharide structure determination by MS is the numerous isomers for a given chemical composition 55,56 . In particular, most of these isomers are stereoisomers, meaning that they are only different in terms of the orientation of their chemical bonds; this makes structural differentiation challenging 55,56 .…”

Section: Mkmentioning

confidence: 99%

“…Part of the difficulty in monosaccharide structure determination by MS is the numerous isomers for a given chemical composition 55,56 . In particular, most of these isomers are stereoisomers, meaning that they are only different in terms of the orientation of their chemical bonds; this makes structural differentiation challenging 55,56 . In this study, untargeted metabolomics analysis was performed using a liquid chromatography-tandem mass spectrometry (LC/MS/MS) approach.…”

Section: Mkmentioning

confidence: 99%

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Enterohaemorrhagic E. coli utilizes host- and microbiota-derived L-malate as a signaling molecule for intestinal colonization

Liu,

Jiang,

Liu

et al. 2023

Nat Commun

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The mammalian gastrointestinal tract is a complex environment that hosts a diverse microbial community. To establish infection, bacterial pathogens must be able to compete with the indigenous microbiota for nutrients, as well as sense the host environment and modulate the expression of genes essential for colonization and virulence. Here, we found that enterohemorrhagic Escherichia coli (EHEC) O157:H7 imports host- and microbiota-derived L-malate using the DcuABC transporters and converts these substrates into fumarate to fuel anaerobic fumarate respiration during infection, thereby promoting its colonization of the host intestine. Moreover, L-malate is important not only for nutrient metabolism but also as a signaling molecule that activates virulence gene expression in EHEC O157:H7. The complete virulence-regulating pathway was elucidated; the DcuS/DcuR two-component system senses high L-malate levels and transduces the signal to the master virulence regulator Ler, which in turn activates locus of enterocyte effacement (LEE) genes to promote EHEC O157:H7 adherence to epithelial cells of the large intestine. Disruption of this virulence-regulating pathway by deleting either dcuS or dcuR significantly reduced colonization by EHEC O157:H7 in the infant rabbit intestinal tract; therefore, targeting these genes and altering physiological aspects of the intestinal environment may offer alternatives for EHEC infection treatment.

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