A Simple Technique for Augmentation of Axonal Ingrowth Into Chondroitinase-Treated Acellular Nerve Grafts Using Nerve Growth Factor

Ovalle, Fernando; Patel, Ashit; Pollins, Alonda C.; Torre, Jorge de la; Vásconez, Luis O.; Hunt, Thomas R.; Bucy, R. Pat; Shack, R. Bruce; Thayer, Wesley P.

doi:10.1097/sap.0b013e3182380974

Cited by 10 publications

(1 citation statement)

References 42 publications

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“…Primary repair is recommended when nerve ends are severed without tension 2–4 ; however, approximately 40% of these surgeries fail and require a secondary surgical procedure to restore sensory and motor function 5 . After repair, nerve regeneration occurs at approximately 1 mm/day, which can result in a lengthy recovery when the injury site is not in close proximity to the neuromuscular junction 6 . Although electromyography and nerve conduction studies (NCS) are the current standards to assess nerve regeneration in extremities 7 , these methods are incapable of monitoring and quantifying nerve recovery until muscles are reinnervated.…”

Section: Introductionmentioning

confidence: 99%

Probabilistic Assessment of Nerve Regeneration with Diffusion MRI in Rat Models of Peripheral Nerve Trauma

Esteve

Farinas

Pollins

et al. 2019

Sci Rep

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Nerve regeneration after injury must occur in a timely fashion to restore function. Unfortunately, current methods (e.g., electrophysiology) provide limited information following trauma, resulting in delayed management and suboptimal outcomes. Herein, we evaluated the ability of diffusion MRI to monitor nerve regeneration after injury/repair. Sprague-Dawley rats were divided into three treatment groups (sham = 21, crush = 23, cut/repair = 19) and ex vivo diffusion tensor imaging (DTI) and diffusion kurtosis imaging (DKI) was performed 1–12 weeks post-surgery. Behavioral data showed a distinction between crush and cut/repair nerves at 4 weeks. This was consistent with DTI, which found that thresholds based on the ratio of radial and axial diffusivities (RD/AD = 0.40 ± 0.02) and fractional anisotropy (FA = 0.53 ± 0.01) differentiated crush from cut/repair injuries. By the 12th week, cut/repair nerves whose behavioral data indicated a partial recovery were below the RD/AD threshold (and above the FA threshold), while nerves that did not recover were on the opposite side of each threshold. Additional morphometric analysis indicated that DTI-derived normalized scalar indices report on axon density (RD/AD: r = −0.54, p < 1e-3; FA: r = 0.56, p < 1e-3). Interestingly, higher-order DKI analyses did not improve our ability classify recovery. These findings suggest that DTI may provide promising biomarkers for distinguishing successful/unsuccessful nerve repairs and potentially identify cases that require reoperation.

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Section: Introductionmentioning

confidence: 99%

Probabilistic Assessment of Nerve Regeneration with Diffusion MRI in Rat Models of Peripheral Nerve Trauma

Esteve

Farinas

Pollins

et al. 2019

Sci Rep

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Mass spectrometry comparison of nerve allograft decellularization processes

Pollins

Kim

Boyer

et al. 2016

J Mater Sci: Mater Med

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Peripheral nerve repair using nerve grafts has been investigated for several decades using traditional techniques such as histology, immunohistochemistry, and electron microscopy. Recent advances in mass spectrometry techniques have made it possible to study the proteomes of complex tissues, including extracellular matrix rich tissues similar to peripheral nerves. The present study comparatively assessed three previously described processing methods for generating acellular nerve grafts by mass spectrometry. Acellular nerve grafts were additionally examined by F-actin staining and nuclear staining for debris clearance. Application of newer techniques allowed us to detect and highlight differences among the 3 treatments. Isolated proteins were separated by mass on polyacrylamide gels serving 2 purposes. This further illustrated that these treatments differ from one another and it allowed for selective protein extractions within specific bands/molecular weights. This approach resulted in small pools of proteins that could then be analyzed by mass spectrometry for content. In total, 543 proteins were identified, many of which corroborate previous findings for these processing methods. The remaining proteins are novel discoveries that expand the field. With this pilot study, we have proven that mass spectrometry techniques complement and add value to peripheral nerve repair studies.

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Recent advances in the therapeutic uses of chondroitinase ABC

Muir

Winter

Verhaagen

et al. 2019

Experimental Neurology

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A Simple Technique for Augmentation of Axonal Ingrowth Into Chondroitinase-Treated Acellular Nerve Grafts Using Nerve Growth Factor

Abstract: The simple technique of doping acellular, chondroitinase-treated nerve grafts with NGF can augment axonal ingrowth and possibly preferentially induce sensory axons.

Cited by 10 publications

References 42 publications

Probabilistic Assessment of Nerve Regeneration with Diffusion MRI in Rat Models of Peripheral Nerve Trauma

Probabilistic Assessment of Nerve Regeneration with Diffusion MRI in Rat Models of Peripheral Nerve Trauma

Mass spectrometry comparison of nerve allograft decellularization processes

Recent advances in the therapeutic uses of chondroitinase ABC

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