A simple test using restricted PCR-amplified mitochondrial DNA to study the genetic structure ofApis mellifera L

Garnery, Lionel; Solignac, Michel; Celebrano, Giovanna; Cornuet, Jean‐Marie

doi:10.1007/bf02125651

Cited by 221 publications

(357 citation statements)

References 12 publications

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“…Despite significant temporal mitochondrial changes detected in the Macaronesian honey bee populations (Muñoz et al, 2013), our present study suggests that nuclear genetic diversity has not varied significantly and has remained relatively low. The low level of genetic diversity observed in this study is consistent with the results reported for other honey bee island populations such as Crete, Sicily and the Balearic Islands (Garnery et al, 1993;Franck et al, 2000;De la Rúa et al, 2003, respectively). It is known, not only for insects but also for mammals, that island populations have lower level of genetic diversity than their continental counterparts (Frankham, 2008) mainly due to genetic drift and differential selection inherent to the colonization process.…”

Section: Discussionsupporting

confidence: 92%

Effects of queen importation on the genetic diversity of Macaronesian island honey bee populations (Apis melliferaLinneaus 1758)

Muñoz

Pinto

Rúa

2014

Journal of Apicultural Research

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SummaryBeekeeping practices such as the importation of non-native honey bee queens may interact with the conservation of honey bee biodiversity.Island honey bee populations are particularly appropriate to test the impact of the introduction of foreign subspecies into their genetic diversity and structure. Here we have used microsatellite markers to evaluate the temporal genetic variation over the last decade in Macaronesian honey bee populations, which have been exposed to different beekeeping strategies regarding queen importation as previously revealed by mitochondrial determination of the evolutionary lineage: a high level on Tenerife and São Miguel and absent or low on La Palma and Madeira. While genetic diversity remained low (from 0.402 to 0.483 in the previous survey and from 0.390 to 0.513 in the current survey), genetic introgression from foreign honey bees was detected on Tenerife and São Miguel based on Bayesian structure analysis.Nevertheless, the existence of endemic honey bee populations deserving conservation on the Macaronesian Islands can still be inferred from the aforementioned analyses. Efecto de la importación de reinas en la diversidad genética de poblaciones de abejas insulares de la Macaronesia (Apis mellifera Linneaus 1758) ResumenPrácticas apícolas como la importación de abejas reinas no-nativas pueden interactuar con la conservación de la biodiversidad de abejas. Las poblaciones de abejas insulares son especialmente adecuadas para analizar el impacto de la introducción de subespecies foráneas en su diversidad y estructura genéticas. En este trabajo hemos utilizado como marcadores los microsatélites para evaluar la variación genética temporal en los últimos diez años en las poblaciones de abejas de la Macaronesia que implementan diferentes estrategias de gestión de la apicultura en relación con la importación de reinas: a un mayor nivel en el caso de Tenerife y San Miguel y ausente o reducida en La Palma y Madeira. Si bien la diversidad genética se ha mantenido baja, (de 0,402 a 0,483 en el muestreo antiguo y de 0,390 a 0,513 en el muestreo actual) se han detectado señales de introgresión genética de abejas foráneas en las poblaciones de Tenerife y San Miguel a partir de los resultados del análisis bayesiano de la estructura poblacional. No obstante, todavía se puede inferir de los análisis mencionados la existencia de poblaciones de abejas endémicas en las islas macaronésicas adecuadas para ser conservadas.

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Section: Discussionsupporting

confidence: 92%

Effects of queen importation on the genetic diversity of Macaronesian island honey bee populations (Apis melliferaLinneaus 1758)

Muñoz

Pinto

Rúa

2014

Journal of Apicultural Research

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“…Restriction with the enzyme Dra I yielded seven different restriction patterns (Fig. 2) (Garnery et al, 1993(Garnery et al, , 1995Franck et al, 1998Franck et al, , 2000aFranck et al, , 2000bFranck et al, , 2001. …”

Section: Haplotypesmentioning

confidence: 99%

“…In particular, mtDNA variance in the COI-COII region has been extensively used to discriminate among the A. mellifera subspecies (Moritz et al, 1994(Moritz et al, , 1998Garnery et al, 1995). Although the majority of African honeybees belong to a mitochondrial lineage termed "A" (Smith, 1991;Garnery et al, 1992Garnery et al, , 1993Sheppard, 1996., Franck et al, 2001) (Garnery et al, 1995). In Egypt A. m. lamarckii only carries the O-lineage haplotypes, whereas A. m. jemenitica from Ethiopia belongs to the Y-lineage detected by Franck et al (2001).…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial discrimination of honeybees (Apis mellifera) of Sudan

El-Niweiri

Moritz

2008

Apidologie

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“…Four evolutionary lineages have been described based on phenotypes and molecular traits (Garnery et al 1992(Garnery et al , 1993Estoup et al 1995;Franck et al 2000Franck et al , 2001Alburaki et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the endemic honeybee: Apis mellifera unicolor (Hymenoptera: Apidae) in Madagascar

et al. 2015

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-Apis mellifera unicolor is a tropical honeybee endemic of Madagascar. Comprehensive knowledge about its mitochondrial and nuclear genetic diversity and structuration was our main purpose. Samples of worker bees were collected from 867 colonies in 76 sites in Madagascar and 1 reference population in South Africa. PCR-restriction fragment length polymorphism (RFLP) and sequencing were used to reveal variability in the COI-COII mtDNA region. Seventeen microsatellite loci were used for studying the nuclear diversity. Three PCR-RFLP profiles were observed, among which 99.4 % belonged to A1 haplotype, 0.2 % to a new A haplotype, and 0.4 % to A4 haplotype. In microsatellite analysis, moderate genetic diversity values were found for Madagascar, together with low mean number of alleles ranging from 2.47 to 3.88 compared to South Africa. Bayesian clustering assignment methods and principal component analysis (PCA) separated populations into two genetic clusters which matched with geographic areas. Several hypotheses are discussed regarding to the low genetic diversity of A. m. unicolor in its native range.Apis mellifera unicolor / microsatellite / African lineage / genetic diversity / mtDNA

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A simple test using restricted PCR-amplified mitochondrial DNA to study the genetic structure ofApis mellifera L

Cited by 221 publications

References 12 publications

Effects of queen importation on the genetic diversity of Macaronesian island honey bee populations (Apis melliferaLinneaus 1758)

Effects of queen importation on the genetic diversity of Macaronesian island honey bee populations (Apis melliferaLinneaus 1758)

Mitochondrial discrimination of honeybees (Apis mellifera) of Sudan

Genetic diversity of the endemic honeybee: Apis mellifera unicolor (Hymenoptera: Apidae) in Madagascar

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