A simple thin layer chromatography based method for the quantitative analysis of biosurfactant surfactin vis-a-vis the presence of lipid and protein impurities in the processing liquid

Dlamini, Betty; Rangarajan, Vivek; Clarke, Kim Gail

doi:10.1016/j.bcab.2020.101587

Cited by 12 publications

(5 citation statements)

References 21 publications

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“…Almost all of them were reactive to ninhydrin and iodine vapors ( Figures 3C , D ), but only some of them were active to UVA and/or UVC ( Figures 3A , B ) with slight activity for UV light but high activity for iodine vapors and ninhydrin ( Figure 3 ). The reactivity to ninhydrin suggests the presence of peptide-natural compounds ( Dlamini et al, 2020 ) and probably molecules that contain unsaturated fatty acids due to reaction with iodine vapors ( Meyers and Meyers, 2008 ). In the case of some UV-active spots, the molecules could include aromatic compounds, such as aromatic amino acids.…”

Section: Discussionmentioning

confidence: 99%

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Chavarria-Quicaño,

Contreras-Jácquez,

Carrillo-Fasio

et al. 2023

Front. Microbiol.

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Phytopathogenic nematodes (PPNs) are responsible for substantial damages within agricultural crops worldwide which can be controlled employing beneficial microorganisms and/or their metabolites in an ecofriendly way. Nevertheless, the success of the control regards not only on the virulence of the strains or the toxicity of their metabolites but also on their ability to colonize and remain in the rhizospheric environment, particularly in those crops affected by abiotic stresses promoted by the climate change. Consequently, the bioprospection of beneficial microorganisms able to control PPNs and to thrive in adverse conditions has attracted attention. On this way, deserts are perfect ecological niches to isolate microorganisms adapted to harsh enviroments. The purpose of this research was to isolate and characterize bacteria from rhizospheric soil samples collected in the Northwestern Desert of Mexico with potential for PPNs control. As first screening, secretomes of each isolate were tested in vitro for nematicidal activity (NA). Then, activities from secretomes and endospores from the selected isolate were confirmed in vivo assays. From 100 thermotolerant isolates, the secretome of the isolate identified as Bacillus paralicheniformis TB197 showed the highest NA (>95%) against Meloidogyne incognita, both in vitro and in vivo tests, suppressing infections caused by M. enterolobii in tomato crops, too. In open field tests, the endospores of TB197 strain showed a reduction of 81% in the infection severity caused by M. enterolobii (p ≤ 0.05), while the galling index (GI) was reduced 84% (p ≤ 0.05) in tomato greenhouse-tests. Also, a reduction of the root necrosis (81%) caused by Radopholus similis in banana plantations (p ≤ 0.05), compared to the control was observed. Owing to their efficacy in controlling PPNs infections, the endospores and secondary metabolites of B. paralicheniformis TB197 strain could be used in bionematicidal formulations.

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Section: Discussionmentioning

confidence: 99%

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Chavarria-Quicaño,

Contreras-Jácquez,

Carrillo-Fasio

et al. 2023

Front. Microbiol.

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“…Para este fin se hizo uso del programa FIJI de ImagenJ win-32 versión 1.44º. El Rf se calculó dividiendo la distancia recorrida por la surfactina u otro componente, en la placa por la distancia recorrida por el frente del solvente y sirvió para identificar el componente en la muestra (Dlamini et al, 2020) Para soporte de los resultados obtenidos con la cromatografía en capa fina, se llevó a cabo una identificación de los principales grupos funcionales por Espectroscopía de Infrarrojo con Transformada de Fourier (FTIR). Las muestras se analizaron en un espectrómetro marca Shimadzu Modelo IRAffinity-1 FT-IR (Shimadzu Scientific Instruments, Columbia, U.S.A.), en modo transmitancia en un rango de número de onda de 400-4000 cm -1 , a una resolución de 4 cm -1 y 50 barridos.…”

Section: Identificación De Lípidos Polares Asociados a Acción Surfact...unclassified

Extraction and characterization of a biosurfactant synthesized by Acinotebacter baumannii

Torres-Custodio¹,

Hernández-Rivera²,

López-Lázaro³

et al. 2023

Mexjbiotechnol

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Biosurfactants are metabolites produced by microorganisms during their growth and reproduction. They are amphiphilic molecules capable of modifying surface and interfacial tension. A petrophilic, nitrogen-fixing, phosphorus-solubilizing, and biosurfactant-producing bacterial strain was isolated and characterized. The isolated strain was cultivated in Kim medium with a C:N ratio of 2:1, from which its growth kinetics was determined. Samples of the culture broth were taken at different times and the collapsed drop, oil dispersion and emulsification index tests were performed. Maximum culture growth occurred at 72 h. The biosurfactant was extracted from the crude cell-free broth using a mixture of solvents and a yield of 2.21 g/L was obtained. Thin-layer chromatography and FT-IR analyzes revealed the presence of a lipopeptide biosurfactant. Tensiometry tests showed that the biosurfactant was able to lower the surface tension of water from 72 mN/m to 40 mN/m and the critical micelle concentration was 0.058 (w/w). Probit analysis for toxicity determination showed that there was no weight loss in Eisenia foetida specimens in a range between 10000 and 50000 ppm.

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“…3). The reactivity to ninhydrin suggests the presence of peptide-natural compounds (Dlamini et al 2020) and probably molecules that contain unsaturated fatty acids due to reaction with iodine vapors (Meyers and Meyers, 2008). In the case of some UV-active spots, the molecules could include aromatic compounds, such as aromatic amino acids.…”

Section: Biological Control In Open-eld Assaysmentioning

confidence: 99%

Bacteria from the Sonoran Desert with nematicidal potential against phytopathogenic nematodes

Chavarria-Quicaño

Contreras-Jácquez

Carrillo-Fasio

et al. 2023

Preprint

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The use of microorganisms and/or their metabolites (e.g., Bacillus, Pseudomonas, etc.) has been proposed as an eco-friendly alternative for controlling phytopathogenic nematodes (PPNs). However, one of the challenges is that microorganisms can thrive in crops affected by climate change, salt stress and drought. Consequently, the search for microorganisms adapted to these conditions has attracted attention. Deserts are characterized by high temperatures, water scarcity, and high salinity, among other harsh conditions; hence, they are a perfect niche for isolating microorganisms adapted to extreme environments. The purpose of this work was to isolate and characterize bacteria from rhizospheric soil samples collected in the Sonoran Desert with the potential for PPN control. Approximately 100 thermotolerant bacteria were isolated, and among them the secretome of the strain identified as Bacillus paralicheniformis TB197 showed the highest nematicidal activity (96%) against Meloidogyne incognita in vitro. Additionally, in field assays, the TB197 secretome effectively suppressed infections caused by M. enterolobii. In greenhouse experiments, TB197 endospores reduced the severity of infection by 81% compared with the control (p≤0.05). Finally, the efficacy of TB197 endospores at the open field level was demonstrated against M. incognita in the tomato crop, where the galling index (GI) was reduced by 84%, and against Radopholus similisin the banana crop, where root necrosis was reduced by 81%, both compared with the controls (p≤0.05). Owing to their efficacy in controlling PNN infections, the endospores and secondary metabolites secreted by the B. paralicheniformis TB197 strain can be used to develop effective bionematicides.

show abstract

A simple thin layer chromatography based method for the quantitative analysis of biosurfactant surfactin vis-a-vis the presence of lipid and protein impurities in the processing liquid

Cited by 12 publications

References 21 publications

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Extraction and characterization of a biosurfactant synthesized by Acinotebacter baumannii

Bacteria from the Sonoran Desert with nematicidal potential against phytopathogenic nematodes

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