A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues

Meezan, Elias; Hjelle, J. Thomas; Brendel, Klaus; Carlson, Edward C.

doi:10.1016/0024-3205(75)90119-8

Cited by 322 publications

(169 citation statements)

References 20 publications

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“…Thoracic aorta obtained from pig (aged 2 months) was rinsed four times for 15 min in phosphate-buffered solution (PBS) containing 1% antibiotic and antimycotic solution (Sigma Chemical, St. Louis, MO) and treated according to Meezan et al (1975) to obtain an acellular matrix. Briefly, samples were frozen and thawed four times and were then processed three times as follows: distilled water for 72 hr at 4°C, 4% sodium deoxycholate (Sigma Chemical) for 4 hr, and 2,000 kU Dnase I (Sigma Chemical) in 1 M NaCl for 2 hr.…”

Section: Materials and Methods Preparation Of Acellular Matrixmentioning

confidence: 99%

Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

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In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a wellknown angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-␤1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-␤1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-␤1 in the acellular matrices.

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Section: Materials and Methods Preparation Of Acellular Matrixmentioning

confidence: 99%

Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

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“…The 20 kidneys were removed and the cortices were dissected into small pieces. The cortices were then sieved according to a standard sieving method, 26 using a series of sieves of 250, 150, and 75 mm (Sansyo, Tokyo, Japan) to isolate the vIL-10 gene transfer in a glomerulonephritis model N Higuchi et al glomeruli, and the GBM was subsequently isolated using the method of Meezan et al 44 Production of NTS containing rabbit polyclonal anti-rat GBM antibodies…”

Section: Preparation Of Rat Gbmmentioning

confidence: 99%

Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar–Kyoto rats

et al. 2003

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Gene therapy is expected to revolutionize the treatment of kidney diseases. Viral interleukin (vIL)-10 has a variety of immunomodulatory properties. We examined the applicability of vIL-10 gene transfer to the treatment of rats with crescentic glomerulonephritis, a T helper 1 (Th 1) predominant disease. To produce the disease, Wistar-Kyoto rats were injected with a rabbit polyclonal anti-rat glomerular basement membrane antibody. After 3 h, a large volume of plasmid DNA expressing vIL-10 (pCAGGS-vIL-10) solution was rapidly injected into the tail vein. pCAGGS solution was similarly injected into control rats (pCAGGS rats). We confirmed the presence of vector-derived vIL-10 mainly in the liver and observed high serum vIL-10 levels in pCAGGSvIL-10-injected rats. Compared with the pCAGGS rats, the pCAGGS-vIL-10 rats showed significant therapeutic effects: reduced frequency of crescent formation, decrease in the number of total cells, macrophages, and CD4 + T cells in the glomeruli, decrease in urine protein, and attenuation of kidney dysfunction. Using quantitative real-time polymerase chain reaction, we also observed that this model was Th1-predominant in the glomeruli and that the ratio of the transcripts of CD4, interferon-g, tumor necrosis factor-a, and monocyte chemotactic protein-1 to the transcripts of glucose-6-phosphate dehydrogenase in the glomeruli were all significantly lower in the pCAGGS-vIL-10 rats than in the pCAGGS rats. These results demonstrate that pCAGGS-vIL-10 gene transfer by hydrodynamics-based transfection suppresses crescentic glomerulonephritis.

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“…The glomeruli were isolated from paired batches of kidney cortex by gentle homogenization and sieving (Krakower and Greenspon 1978). The glomerular basement membrane (GBM) was isolated by the method of Meezan et al (1976).…”

Section: Biochemical Analysismentioning

confidence: 99%

Accumulation of Advanced Glycation Endproducts in the Rat Nephron: Link with Circulating AGEs During Aging

Verbeke

Perichon

Borot–Laloi

et al. 1997

J Histochem Cytochem.

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SUMMARYThe accumulation of advanced glycosylation end products (AGEs) is believed to be a factor in the development of aging nephropathy. We have attempted to establish a link between the formation of AGEs and the onset of renal impairment with aging, indicated by albuminuria, using a fluorescence assay and immunohistochemical detection of AGEs in the renal extracellular matrix in rats. The fluorescence of collagenase-digested Type IV collagen from GBM increased with age, from 1.65 Ϯ 0.05 AU/mM OHPro (3 months) and 1.58 Ϯ 0.04 (10 months

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A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues

Cited by 322 publications

References 20 publications

Angiogenic response induced by acellular aortic matrix in vivo

Angiogenic response induced by acellular aortic matrix in vivo

Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar–Kyoto rats

Accumulation of Advanced Glycation Endproducts in the Rat Nephron: Link with Circulating AGEs During Aging

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