A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment

His, Édouard; Seaman, Matthias; Beiras, Ricardo

doi:10.1016/s0043-1354(96)00244-8

Cited by 179 publications

(115 citation statements)

References 19 publications

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“…The CO 2 group larva in (e) showed limited mineralization in its shell, while the one in (g) showed no mineralization at all. Scale bar = 50 µm fertilization (His et al 1997). However, Thain (1991) reported that gonadal stripping of adult bivalves could give lower percentages of normal embryos as compared with spontaneous production.…”

Section: Discussionmentioning

confidence: 99%

“…Morphological criteria proposed by His et al (1997) were adopted to differentiate 'normal' (D-shaped) from 'abnormal' larvae. To determine the effects of CO 2 on shell growth, larvae that were assessed to be normal were mounted on a glass slide, photographed, and measured for shell length (the anterior-posterior dimension of the shell parallel to the hinge line, Waller 1981) and height (the dorsal-ventral dimension perpendicular to the hinge) under a microscope with an ocular micrometer.…”

Section: Methodsmentioning

confidence: 99%

“…Oysters are ecosystem engineers providing habitats for many rocky-shore benthic organisms, and also have a high commercial value as food. In addition, eggs, embryos and larvae of C. gigas are among the most commonly used organisms for assessing toxicity of pollutants and for evaluating water quality in coastal areas (Calabrese 1984, His et al 1997. We incubated oyster eggs under an elevated pCO 2 condition (seawater pH 7.4) up to, and including, the veliger stage, and quantified the effects on early development including shell mineralization.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of increased seawater pCO2 on early development of the oyster Crassostrea gigas

Kurihara¹,

Kato²,

Ishimatsu³

2007

Aquat. Biol.

294

214

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This study demonstrated that the increased partial pressure of CO 2 (pCO 2 ) in seawater and the attendant acidification that are projected to occur by the year 2300 will severely impact the early development of the oyster Crassostrea gigas. Eggs of the oyster were artificially fertilized and incubated for 48 h in seawater acidified to pH 7.4 by equilibrating it with CO 2 -enriched air (CO 2 group), and the larval morphology and degree of shell mineralization were compared with the control treatment (air-equilibrated seawater). Only 5% of the CO 2 group developed into normal 'Dshaped' veliger larvae as compared with 68% in the control group, although no difference was observed between the groups up to the trochophore stage. Thus, during embryogenesis, the calcification process appears to be particularly affected by low pH and/or the low CaCO 3 saturation state of high-CO 2 seawater. Veliger larvae with fully mineralized shells accounted for 30% of the CO 2 -group larvae, compared with 72% in the control (p < 0.005). Shell mineralization was completely inhibited in 45% of the CO 2 -group larvae, but only in 16% of the control (p < 0.05). Normal D-shaped veligers of the control group exhibited increased shell length and height between 24 and 48 h after fertilization, while the few D-shaped veligers of the CO 2 group showed no shell growth during the same period. Our results suggest that future ocean acidification will have deleterious impacts on the early development of marine benthic calcifying organisms.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of increased seawater pCO2 on early development of the oyster Crassostrea gigas

Kurihara¹,

Kato²,

Ishimatsu³

2007

Aquat. Biol.

294

214

View full text Add to dashboard Cite

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“…Fertilization success was checked under microscope, and embryos were then counted and transferred to 24-well microplates (Greiner Bio-One, Cellstar) for embryotoxicity assays. The embryotoxicity assay protocol has been described in details by His et al (1997) and Quiniou et al (2007) and the standardized AFNOR procedure (AFNOR XP-T90-382 1 ) published in 2009. Fertilized eggs (around 500) were exposed in wells containing 2 ml of toxicant solution.…”

Section: Embryotoxicity Assaymentioning

confidence: 99%

Do Temporal and Spatial Parameters or Lifestyle of the Pacific Oyster Crasssostrea gigas Affect Pollutant Bioaccumulation, Offspring Development, and Tolerance to Pollutants?

et al. 2017

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This study evaluated the vulnerability of early life stages of native oysters (Crassostrea gigas) from the Arcachon Bay (SW, France) to pollutants at risk in the lagoon in particular copper and S-metolachlor. Developmental abnormalities in wild and cultivated oyster D-larvae were investigated during 2 breeding-seasons (2013 and 2014) at different sampling sites and dates. In addition, copper, and metolachlor concentrations were determined both in seawater and in mature oysters. Bioaccumulation of Cu was observed at higher levels in wild than in farmed specimens. Metolachlor was accumulated at much lower levels. After 24 h exposure, significant increases of the percentage of abnormal D-larvae were observed when exposed at 1 µg L −1 of copper or 10 ng L −1 of metolachlor in comparison with the controls whatever the date, the site, and rearing conditions of the genitors. The current study demonstrates that environmental concentrations of copper and metolachlor can induce a significant increase of developmental abnormalities in farmed and wild populations of oysters. However, no significant differences of sensitivity were observed according to temporal, spatial parameters, and lifestyle of genitors. In addition, oyster larvae obtained from the hatchery displayed the same sensitivity to pollutants as larvae from the field, demonstrating their suitability for toxicity assays and water quality monitoring.

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“…The bioassay was based on the methods described by His et al (1997). Briefly, animals were cleaned and scrubbed in natural filtered seawater.…”

Section: Embryo-larval Acute Toxicity Assay Using the Mussel Mytilus mentioning

confidence: 99%

Toxicity assessment of crude and partially purified extracts of marine Synechocystis and Synechococcus cyanobacterial strains in marine invertebrates

Martíns

Fernández

Beiras

et al. 2007

Toxicon

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Among the Cyanoprokaryota, the genera Synechocystis and Synechococcus have rarely been studied with respect to potential toxicity. This is particularly true with marine environments where studies about the toxicity of cyanobacteria are restricted to filamentous forms at the warmer temperate and tropical regions and also to filamentous forms at cold seas such as the Baltic Sea. In this study, we describe the effects of cyanobacterial strains of the Synechocystis and Synechococcus genera isolated from the marine coast of Portugal, on marine invertebrates. Crude and partially purified extracts at a concentration of 100 mg/ml of freeze-dried material of the marine strains were tested for acute toxicity in nauplii of the brine shrimp Artemia salina, in the rotifer Brachionus plicatillis and in embryos of the sea urchin Paracentrotus lividus and the mussel Mytilus galloprovincialis. The cyanobacterial extracts, especially the crude extract, had an impact on A. salina nauplii. No significant toxic effects were registered against the rotifer. A negative impact of all strains was recorded on the embryonic development of the sea urchin, with toxic effects resulting in an inhibition of embryogenesis or development of smaller larvae. To the mussel embryos, the effects of cyanobacterial extracts resulted in a complete inhibition of embryogenesis. The results of all assays indicate that Synechocystis and Synechococcus marine strains contained toxic compounds to marine invertebrates.

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A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment

Cited by 179 publications

References 19 publications

Effects of increased seawater pCO2 on early development of the oyster Crassostrea gigas

Effects of increased seawater pCO2 on early development of the oyster Crassostrea gigas

Do Temporal and Spatial Parameters or Lifestyle of the Pacific Oyster Crasssostrea gigas Affect Pollutant Bioaccumulation, Offspring Development, and Tolerance to Pollutants?

Toxicity assessment of crude and partially purified extracts of marine Synechocystis and Synechococcus cyanobacterial strains in marine invertebrates

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