A simplified analysis of fatty acids in serum phospholipids using Sep-Pak cartridges

Ellin, Åke; Berg, S. Van Den; Strandvik, Birgitta

doi:10.1016/0009-8981(91)90336-b

Cited by 15 publications

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phospholipid fatty acids have been measured in most epidemiological studies, but there is some evidence that total plasma fatty acids are better correlated with dietary intakes [30]. Phospholipid fatty acids were isolated by placing the chloroform extract on a silica cartridge, washing with chloroform and methanol before eluting with chloroform:methanol:water (3:5:2) based on published methods [31]. The internal standard, 17:0, was added to the phospholipid eluate and the total lipid chloroform extract, and each fraction was dried.…”

Section: Methodsmentioning

confidence: 99%

A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters

Djurić¹,

Ren²,

Blythe³

et al. 2009

Nutrition Research

View full text Add to dashboard Cite

This study examined patterns of changes in plasma fatty acids and carotenoids when women were asked to follow a novel, Greek-Mediterranean exchange list diet. A total of 69 healthy, non-obese, women ages 25–59, were randomized to either continue their own usual diet or to follow a modified Mediterranean diet for six months. There were no significant changes in blood lipids, triacylglycerol, insulin, glucose or C-reactive protein. Mean plasma carotenoids increased by 55%, which is consistent with a large increase in fruit and vegetable consumption. Likewise, changes in fat intakes were reflected in blood fatty acids, with a 25% increase in mean plasma monounsaturated fatty acids (MUFA). Principal component analysis was conducted to examine the sources of inter-individual variation for changes in carotenoid and fatty acid levels. Changes in the Mediterranean diet were clustered together in four components that accounted for 78% of the variance in plasma levels. Increases in plasma lutein, α- and β-carotene clustered together in a “vegetable” pattern, and increases in carotenoids found in fruit, β-cryptoxanthin and zeaxanthin, also clustered together but accounted for less of the variance. Increases in plasma MUFA were clustered with a decrease in plasma polyunsaturated fatty acids, consistent with substitution in the type of oils consumed. The only association of fatty acid levels with carotenoids was that of lycopene, which clustered together with an increase in saturated fatty acids. The changes in blood levels indicate the exchange list diet was effective for targeting Mediterranean nutrient intakes using foods available in the United States.

show abstract

Section: Methodsmentioning

confidence: 99%

A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters

Djurić¹,

Ren²,

Blythe³

et al. 2009

Nutrition Research

View full text Add to dashboard Cite

show abstract

“…21,22 The fatty acid pattern of serum phospholipids was analyzed as previously reported. 23 According to Menghini,24 liver biopsy was performed under general anesthesia in patients younger than 16 years and under local anesthesia in older patients. The biopsy specimen was prepared according to routine methods and stained with hematoxylin-eosin, periodic acid-Schiff Ϯ diastase treatment, reticulin, and iron stains.…”

mentioning

confidence: 99%

Natural history of liver disease in cystic fibrosis

1999

View full text Add to dashboard Cite

The median age of the population with cystic fibrosis (CF) has increased worldwide, which has led to the suggestion that the prevalence of liver disease would increase. The aim of this study was to evaluate the natural history of CF‐associated liver disease over a 15‐year period in a well‐controlled population of patients with CF. During the years 1976 through 1993, 124 patients were followed up by yearly liver function tests (LFTs). Fifteen patients were followed up with liver biopsies throughout the whole study period. More than 50% of the patients had pathological LFTs in infancy, later being normalized. Approximately 25% of children 4 years of age or older had biochemical markers of liver disease during the study period. In about 10% of the patients, cirrhosis or advanced fibrosis was confirmed at biopsy and 4% of patients had cirrhosis with clinical liver disease. Severe liver disease developed mainly during prepuberty and puberty. Of the 15 patients prospectively followed up with liver biopsies, only 3 had progressive fibrosis. No specific risk factor was identified, but deficiency of essential fatty acids was found more often in patients with marked steatosis (P < .05). No patient developed clinical liver disease in adulthood and the histological changes in the liver biopsies were usually not progressive. Liver disease was no more frequent at the end of the study period although the median age of the patient population had increased. Modern treatment might positively influence liver disease because it seemed less common, less progressive, and less serious than previously reported.

show abstract

“…Briefly, plasma was extracted with hexane for analysis of carotenoids by HPLC with electrochemical detection [24]. Fatty acids were analyzed in plasma phospholipids using Folch extraction of plasma and phospholipid isolation using sep-paks [25]. Derivatization was with Methanolic KOH, using dry conditions, which esterifies all classes of fatty acids well [26].…”

Section: Methodsmentioning

confidence: 99%

Obesity is associated with atypia in breast ductal lavage of women with proliferative breast disease

Djurić

Edwards

Madan

et al. 2009

Cancer Epidemiology

View full text Add to dashboard Cite

Background-Benign proliferative breast disease without atypia slightly increases breast cancer risk but there are currently few clinical options for breast cancer prevention in this group of women.Methods-We conducted a pilot study of women with a past diagnosis of proliferative breast disease with a goal to determine if the characteristics of cells obtained by breast ductal lavage were related to nutritional factors.Results-There were 57 women who enrolled. A total of 39 women yielded nipple aspirate fluid (NAF) samples and 36 underwent breast ductal lavage. Five of the lavage samples were acellular and 28 had at least 200 cells. Surprisingly, atypia was present in 11 women. Presence of atypia was associated with slight changes in morphometric features of the epithelial cells such as measures of circularity) as obtained by image analysis, but the only variable significantly different in women with atypia (versus no atypia) was a higher mean body mass index. Body mass index also was significantly correlated with C-reactive protein (CRP) levels in the nipple aspirate fluid, indicating that obesity might have a pro-inflammatory effect on the breast that can contribute to increased rates of atypia.Conclusions-Although the clinical significance of atypia in breast ductal lavage is uncertain, these results support further work on prevention of obesity as a strategy for reducing breast cancer risk. Keywordsproliferative breast disease; nuclear morphometry; breast ductal lavage; obesity

show abstract

A simplified analysis of fatty acids in serum phospholipids using Sep-Pak cartridges

Cited by 15 publications

References 2 publications

A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters

A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters

Natural history of liver disease in cystic fibrosis

Obesity is associated with atypia in breast ductal lavage of women with proliferative breast disease

Contact Info

Product

Resources

About