A simplified laminin nomenclature

Aumailley, Monique; Bruckner‐Tuderman, Leena; CARTER, W; Deutzmann, Rainer; Edgar, David; Ekblom, Peter; Engel, Jürgen; Engvall, Eva; Hohenester, Erhard; Jones, Jonathan

doi:10.1016/j.matbio.2005.05.006

Cited by 785 publications

(668 citation statements)

References 6 publications

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“…Samples were then stored at −20°C until insulin and DNA measurement (see below). In separate experiments, soluble laminin (LM)-111 (α1β1γ1; according to new LM nomenclature [20]) (30 μg/ml) (BD), neutralising antibodies to thrombospondin-1 (TSP-1; 1 μg/ml) (LabVision, Fremont, CA, USA), hepatocyte growth factor (HGF; 1 μg/ml) (R&D Systems, Minneapolis, MN, USA) or the β1-chain of LM (18 μg/ml; Clone 3E5, Catalogue number MAB2041; Millipore, Solna, Sweden), or the endothelin A (ET A ) and endothelin B (ET B ) receptor antagonists BQ-123 (10 μmol/l) and BQ-788 (1 μmol/l) (Bachem UK, St Helens, UK), were added to the high-glucose medium.…”

Section: Isolation Of Islet Endothelial Cellsmentioning

confidence: 99%

Endothelial cell signalling supports pancreatic beta cell function in the rat

et al. 2009

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Aims/hypothesis The proximity of endothelial cells and beta cells in islets by necessity means that they are exposed to each other's products. Whereas islet endothelial cells require signals from beta cells to function properly, endothelin-1, thrombospondin-1 and laminins, among others, have been identified as endothelial-derived molecules, although their full effects on beta cells have not been explored. We tested the hypothesis that islet endothelialderived products affect beta cell function. Methods Endothelial cells from rat islets were proliferated and purified. Endothelium-conditioned culture medium (ECCM) was obtained by maintaining the endothelial cells in culture medium. Islet function was evaluated following exposure of cultured islets to standard culture medium or ECCM. Changes in mRNA levels for key beta cell metabolic enzymes were also measured in islets after ECCM exposure.Results Glucose-stimulated insulin release and islet insulin content were markedly enhanced by exposure to ECCM. This was at least partly explained by improved mitochondrial function, as assessed by glucose oxidation and an upregulation of the mitochondrial gene for glycerol-3-phosphate dehydrogenase (mGpdh [also known as Gpd2]), combined with upregulation of the rate-limiting enzyme in the glycolysis, glucokinase, in the islets. The intracellular degradation of insulin was also decreased in the islets. Islet endothelial cells produced laminins, and the positive effects of islet endothelial cells were prevented by addition of a neutralising antibody to the β1-chain of laminin. Addition of exogenous laminin stimulated islet function. Conclusions/interpretation This study provides proof of principle that endothelial cells can affect the function of beta cells in their vicinity and that this is at least partially mediated by laminins.

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Section: Isolation Of Islet Endothelial Cellsmentioning

confidence: 99%

Endothelial cell signalling supports pancreatic beta cell function in the rat

et al. 2009

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“…To date, 16 different types of laminin trimers have been identified. 8 Modulation of specific forms of laminin or their cell surface receptors may play an important role in snail-mediated metastasis. Laminin-332 (laminin-5) has been reported to bind strongly to integrin α6β4 and to form hemidesmosomes.…”

Section: The Role Of Snail In Cell Detachment From Extracellular Matrmentioning

confidence: 99%

The role of the transcriptional regulator snail in cell detachment, reattachment and migration

Haraguchi

2009

Cell Adhesion & Migration

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“…It plays critical roles in the maintenance of tissue architectures and the regulation of cellular functions such as cell adhesion, migration, proliferation, apoptosis, and differentiation (1). Different combinations of five ␣, three ␤, and three ␥ chains give rise to more than 15 laminin isoforms (2). These laminins are distributed in a tissue-specific manner and are thought to play differential roles in the individual tissues.…”

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Laminin-3B11, a Novel Vascular-type Laminin Capable of Inducing Prominent Lamellipodial Protrusions in Microvascular Endothelial Cells

Mori

Ono

Kariya

et al. 2010

Journal of Biological Chemistry

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The basement membrane (BM) proteins laminins, which consist of ␣, ␤, and ␥ chains, support tissue structures and cellular functions. To date only ␣4 and ␣5 types of laminins have been identified in the BMs of blood vessels. Our recent study suggested the presence of novel ␣3B-containing laminins in vascular BMs. Here we identified and characterized the third member of vascular laminins, laminin-3B11 (Lm3B11). RT-PCR analysis showed that microvascular endothelial (MVE) cells and umbilical vein endothelial cells expressed the messages for the ␣3B, ␤1, ␤2, and ␥1 chains. In the culture of MVE cells, ␣3B was associated with ␤1 and ␥1, producing Lm3B11. Recombinant Lm3B11 was overexpressed by introducing the cDNAs of the three chains into HEK-293 cells and purified to homogeneity. Purified Lm3B11 exhibited relatively weak cell adhesion activity through both ␣3␤1 and ␣6␤1 integrins. Most characteristically, Lm3B11 strongly stimulated MVE cells to extend many lamellipodial protrusions. This pseudopodial branching was blocked by an inhibitor for Src or phosphatidylinositol 3-kinase. Consistently, Lm3B11 stimulated the phosphorylation of Src and Akt more strongly than other laminins, suggesting that the integrinderived signaling is mediated by these factors. The unique activity of Lm3B11 appears to be favorable to the branching of capillaries and venules.Laminin, a family of large heterotrimeric glycoproteins consisting of ␣, ␤, and ␥ chains, is a major cell-adhesive component present in the basement membranes (BMs) 2 of various tissues. It plays critical roles in the maintenance of tissue architectures and the regulation of cellular functions such as cell adhesion, migration, proliferation, apoptosis, and differentiation (1). Different combinations of five ␣, three ␤, and three ␥ chains give rise to more than 15 laminin isoforms (2). These laminins are distributed in a tissue-specific manner and are thought to play differential roles in the individual tissues. Such specific distribution and function of laminins are mainly determined by the ␣ chains. For example, ␣2 laminins (laminin-211 and -221) are localized in the BMs of muscle and neurons, whereas ␣4 laminins (laminin-411 and -421) are localized in vascular BMs.There are two splicing variants of laminin ␣3 chain, the truncated form ␣3 (or ␣3A) and the full-sized form ␣3B (3, 4), gene expression of which is regulated by alternative splicing and different promoters (5). Laminin-3A32 (Lm3A32), which is previously known as laminin-5 and consists of all truncated chains (␣3A, ␤3, and ␥2), is a major laminin isoform in the skin, esophagus, lung, breast, and other epithelial tissues. This laminin has extensively been studied because it has unique structure and biological activity as compared with other laminins (6). In the skin, Lm3A32 in the BM associates with integrin ␣6␤4 of basal keratinocytes to form the hemidesmosome structures and contributes to their stable anchorage to the underlying connective tissues (7,8). The laminin ␣3A chain also associates with the full-siz...

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A simplified laminin nomenclature

Cited by 785 publications

References 6 publications

Endothelial cell signalling supports pancreatic beta cell function in the rat

Endothelial cell signalling supports pancreatic beta cell function in the rat

The role of the transcriptional regulator snail in cell detachment, reattachment and migration

Laminin-3B11, a Novel Vascular-type Laminin Capable of Inducing Prominent Lamellipodial Protrusions in Microvascular Endothelial Cells

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