A simplified method for selecting a carbon-fiber electrode in pulse voltammetry

Liao, Bin-Kai; Lio, H.-P.; Wang, C.-Y.; Young, Ming‐Shing; Ho, Ming-Tzu; Lin, Mao‐Tsun

doi:10.1016/0165-0270(93)90036-q

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…Auxiliary (silver wire) and reference (Ag/AgCl) electrodes were placed on the dura surface of the parietal bone. Differential pulse voltamograms were recorded automatically every 25 s as detailed previously [1,14–16]. After holes had been drilled in the parietal bones, two self‐tapping screws were attached through the appropriate holes.…”

Section: Methodsmentioning

confidence: 99%

Melatonin potentiates 5‐HT_1A receptor activation in rat hypothalamus and results in hypothermia

Lin

Chuang

2002

Journal of Pineal Research

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Therapeutic hypothermia is widely applied as a neuroprotective measure on intracerebral hemorrhage (ICH). However, several clinical trials regarding physical hypothermia encountered successive failures because of its side-effects in recent years. Increasing evidences indicate that chemical hypothermia that targets hypothalamic 5-HT1a has potential to down-regulate temperature set point without major side-effects. Thus, this study examined the efficacy and safety of 5-HT1a stimulation in PO/AH area for treating ICH rats. First, the relationship between head temperature and clinical outcomes was investigated in ICH patients and rat models, respectively. Second, the expression and distribution of 5-HT1a receptor in PO/AH area was explored by using whole-cell patch and confocal microscopy. In the meantime, the whole-cell patch was subsequently applied to investigate the involvement of 5-HT1a receptors in temperature regulation. Third, we compared the efficacy between traditional PH and 5-HT1a activation-induced hypothermia for ICH rats. Our data showed that more severe perihematomal edema (PHE) and neurological deficits was associated with increased head temperature following ICH. 5-HT1a receptor was located on warm-sensitive neurons in PO/AH area and 8-OH-DPAT (5-HT1a receptor agonist) significantly enhanced the firing rate of warm-sensitive neurons. 8-OH-DPAT treatment provided a steadier reduction in brain temperature without a withdrawal rebound, which also exhibited a superior neuroprotective effect on ICH-induced neurological dysfunction, white matter injury and BBB damage compared with physical hypothermia. These findings suggest that chemical hypothermia targeting 5-HT1a receptor in PO/AH area could act as a novel therapeutic manner against ICH, which may provide a breakthrough for therapeutic hypothermia.

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Section: Methodsmentioning

confidence: 99%