A simplified method for the preparation of rotifers for transmission and scanning electron microscopy

Amsellem, Jacqueline; Cl�ment, Pierre

doi:10.1007/bf00019433

Cited by 18 publications

(3 citation statements)

References 6 publications

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“…6). In the stages we examined, we did not detect lophotrochin expression in other cells bearing cilia: for example, the two ventral-lateral antennae which include a cluster of cilia 51 .…”

Section: Resultsmentioning

confidence: 74%

Genes with spiralian-specific protein motifs are expressed in spiralian ciliary bands

et al. 2020

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Spiralia is a large, ancient and diverse clade of animals, with a conserved early developmental program but diverse larval and adult morphologies. One trait shared by many spiralians is the presence of ciliary bands used for locomotion and feeding. To learn more about spiralianspecific traits we have examined the expression of 20 genes with protein motifs that are strongly conserved within the Spiralia, but not detectable outside of it. Here, we show that two of these are specifically expressed in the main ciliary band of the mollusc Tritia (also known as Ilyanassa). Their expression patterns in representative species from five more spiralian phyla-the annelids, nemerteans, phoronids, brachiopods and rotifers-show that at least one of these, lophotrochin, has a conserved and specific role in particular ciliated structures, most consistently in ciliary bands. These results highlight the potential importance of lineage-specific genes or protein motifs for understanding traits shared across ancient lineages.

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“…6). In the stages we examined, we did not detect lophotrochin expression in other cells bearing cilia: for example, the two ventral-lateral antennae which include a cluster of cilia 51 .…”

Section: Resultsmentioning

confidence: 74%

Genes with spiralian-specific protein motifs are expressed in spiralian ciliary bands

et al. 2020

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“…Scanning electron microscopy (SEM) was performed on several complete specimens, resting eggs and trophi from each of the three species using a Hitachi S-4100 microscope. Complete animals and resting eggs were prepared as in Ansellem and Clément (Ansellem and Clément, 1980) with minor modifications. Trophi were prepared for SEM according to De Smet (De Smet, 1998).…”

Section: Morphologymentioning

confidence: 99%

On the taxonomy of three sympatric sibling species of the Brachionus plicatilis (Rotifera) complex from Spain, with the description of B. ibericus n. sp.

Ciros‐Pérez

Gómez²,

Serra³

2001

Journal of Plankton Research

158

130

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“…Selected individuals were observed under a scanning electron microscope (Cambridge S 120), having been previously subjected to dehydration (alcohol chain, critica1 point), and coating gold (sputtering method). (AMSELLEM & CLEMENT, 1980).…”

Section: Methodsmentioning

confidence: 99%

Contribution to the knowledge of the rotifer fauna (Rotifera: Monogononta) from Minorcan inland waters (Balearic isles: Spain)

Manuel¹

1990

Limnetica

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Forty-four species of rotifers have been identified in Minorca inland waters. Permanent ponds, temporary pools, flowing waters, and artificial pools were sampled. Most of the species are new records for the island and twentynine are new to the Balearic Isles. The families Brachionidae and Lecanidae provide half of the taxa. Keratella procurva (Thorpe, 1912), which is considered a subtropical species, occurred with a widespread distnbution in the island. Different taxa and «Formenkreis» found are mentioned. Original drawings and scanning photomicrographs of some species are presented. Typical assemblages of populations from every environment are also described.

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A simplified method for the preparation of rotifers for transmission and scanning electron microscopy

Abstract: Tor TEM and SEM prepatations, rotifers are placed in little panels made of plastic Beem capsules normally used for embedding, with their conical parts cut off and closed by plankton filter cloth. Thus, the risk of losing animals is considerably reduced. Methods

Cited by 18 publications

References 6 publications

Genes with spiralian-specific protein motifs are expressed in spiralian ciliary bands

Genes with spiralian-specific protein motifs are expressed in spiralian ciliary bands

On the taxonomy of three sympatric sibling species of the Brachionus plicatilis (Rotifera) complex from Spain, with the description of B. ibericus n. sp.

Contribution to the knowledge of the rotifer fauna (Rotifera: Monogononta) from Minorcan inland waters (Balearic isles: Spain)

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