A Simplified Method to Attach Antibodies on Liposomes by Biotin-Streptavidin Affinity for Rapid and Economical Screening of Targeted Liposomes

Papadia, Konstantina; Markoutsa, Eleni; Antimisiaris, Sophia G.

doi:10.1166/jbn.2014.1792

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…Recently, the fabrication of the surface-modified lipidic vesicles was also proposed, decorating the external surfaces with labels such as peptides [89], opsonines, antibodies [90] or polymer fragments [91] to obtain a specific long-lasting drug release to target tissues, creating special bindings with cell receptors [30].…”

Section: Natural and Artificial Release Mechanismsmentioning

confidence: 99%

Liposomes: From Bangham to Supercritical Fluids

2020

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Liposomes are spherical vesicles made up of an aqueous core surrounded by phospholipids. These delivery systems (DS) are largely employed as drug carriers in several industrial fields, such as pharmaceutical and nutraceutical fields. The aim of this short review is to provide a fast overview on the main fundamentals of liposomes, thought as a compact guide for researchers and students that want to approach this topic for the first time. The mini-review will focus on the definitions, production methods and characterization protocols of the liposomes produced, making a critical comparison of the main conventional and supercritical based manufacturing methods available. The literature was analyzed deeply from the first works by Dr. Bangham in 1965 to the most recent supercritical fluid applications. The advantages and disadvantages of conventional and high-pressure processes will be described in terms of solvent elimination, production at the nanometric (50–300 nm) and micrometric level (1–100 μm) and encapsulation efficiency (20–90%). The first proposed methods were characterized by a low encapsulation efficiency (20–40%), resulting in drug loss, a high solvent residue and high operating cost. The repeatability of conventional processes was also low, due to the prevalent batch mode. Supercritical-assisted methods were developed in semi-continuous layouts, resulting in an easy process scale-up, better control of liposome dimensions (polydispersity index, PDI) and also higher encapsulation efficiencies (up to 90%).

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Section: Natural and Artificial Release Mechanismsmentioning

confidence: 99%

Liposomes: From Bangham to Supercritical Fluids

2020

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“…This method has been utilized with targeting antibodies such as anti‐HER2 or anti‐My9, to create targeted liposomes . Other potential chemistries include click chemistry and avidin/biotin chemistries that have previously been employed with liposome based carriers and could readily be translated to protocells …”

Section: Modular Design and Combined Functions Of Protocellsmentioning

confidence: 99%

Protocells: Modular Mesoporous Silica Nanoparticle‐Supported Lipid Bilayers for Drug Delivery

Butler

Durfee

Théron

et al. 2016

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126

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Mesoporous silica nanoparticle supported-lipid bilayers, termed ‘protocells,’ represent a potentially transformative class of therapeutic and theranostic delivery vehicles. The field of targeted drug delivery poses considerable challenges that cannot be addressed with a single ‘magic bullet’. Consequently the protocell has been designed as a modular platform composed of interchangeable biocompatible components. The mesoporous silica core can have variable size and shape to direct biodistribution and controlled pore size and surface chemistry to accommodate diverse cargos. The encapsulating supported lipid bilayer can be modified with targeting and trafficking ligands as well as PEG to effect selective binding, endosomal escape of cargo, drug efflux prevention, and potent therapeutic delivery, while maintaining in vivo colloidal stability. This mini-review describes the individual components of the platform, including the mesoporous silica nanoparticle core and supported lipid bilayer, their assembly (by multiple techniques) into a protocell, and the combined, often synergistic, performance of the protocell based on in vitro and in vivo studies including assessment of biocompatibility and toxicity. We close by commenting on the many emerging variations of the protocell theme and the future directions for protocell research.

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“…Nonetheless, a two-step active targeting is also possible and it was the case for molecular imaging of delineating tumor margins in a C6 glioma-bearing model [127]. Herein, the biotin-streptavidin ligation technique was used for its reability to attach antibodies on liposomes surface [133]. Gadolinium was used as the imaging agent and endoglin (CD105), a protein involved in angiogenesis, was used as the targeting moiety.…”

Section: Cancermentioning

confidence: 99%

Liposomal Drug Delivery to the Central Nervous System

Nieto-Montesinos¹

2017

Liposomes

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Central nervous system diseases represent a huge world of burden of human suffering with negative economic results. Most therapeutic compounds cannot attain the brain because of the blood-brain barrier and its expression of efflux transporters. Among them, the P-glycoprotein plays a significant role leading to failure of various clinical treatments. A non-invasive strategy to circumvent the blood-brain barrier and P-glycoprotein emphasizes on the encapsulation and therefore masking of therapeutic compounds in drug delivery systems. Up to now, liposomes are the most widely studied drug delivery systems due to their biocompatibility, biodegradability, and less toxicity. The incorporation of polyethylene glycol-lipid derivatives within the bilayer of conventional liposomes significantly prolongs liposomal cargo half-life by steric stabilization. Interestingly, an increased brain accumulation of liposomal cargo is achieved by coupling targeting moieties on liposomes surface. These targeting moieties such as peptides or monoclonal antibodies recognize the biochemical transport systems at the blood-brain barrier and mediate the transport of liposomes and their cargo across this barrier. Moreover, stimuli-sensitive liposomes are programmed for cargo release when exposed to a particular microenvironment. Hence, this chapter highlights the potential liposomal applications for delivery of therapeutic compounds as well as diagnostic tools or both, in major central nervous system diseases.

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A Simplified Method to Attach Antibodies on Liposomes by Biotin-Streptavidin Affinity for Rapid and Economical Screening of Targeted Liposomes

Cited by 10 publications

References 13 publications

Liposomes: From Bangham to Supercritical Fluids

Liposomes: From Bangham to Supercritical Fluids

Protocells: Modular Mesoporous Silica Nanoparticle‐Supported Lipid Bilayers for Drug Delivery

Liposomal Drug Delivery to the Central Nervous System

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