Tire wear quantity and difference is one of the main points that affect the intension of tire road print feature and is under a tight link not only with tire road friction energy but also with vehicle dynamics. The proposed methodology aims to provide a mathematical method to analyze tire marks' feature for prediction the vehicle operating status in pro-and post-crash phases. The kinetic sliding friction coefficient considering the road effect is simplified with the new real contact area model. After, a simplified 9 degrees of freedom vehicle dynamics model by coupling the tire dynamic model is set up, and the effectiveness of the model is evaluated with vehicle stability measurement equipment. Furthermore, a theoretical model of tire wear quantity and difference as a function of road properties and vehicle dynamics is set up combined with vehicle-road coupling algorithm, and important parameters (e.g. vehicle speed, steering angle, braking force, and road feature) are analyzed to assess the tire wear quantity and difference feature when the vehicle is in unsteady condition. Results show that vehicle speed, braking force, and road surface feature have a significant impact to tire wear quantity and difference, the right rear exhibits a fluctuation and the tire wear quantity and difference between rear axle and the right side is obvious, and lay a strong foundation for tire road print analysis.