A simplified new method for the identification of arboviruses: Virus detection using enzyme immunoassay on cultured cells

Zhang, Yonghe; Wenfang, Yu; Zhong-Wen, Tian; Ge, Ji-Qian; Qin-Sheng, Chen; Wang, Yimin

doi:10.1016/0166-0934(84)90082-x

Cited by 12 publications

(2 citation statements)

References 7 publications

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“…The execution of studies designed to elucidate the biology of rodent virus infections requires the ability to detect and quantify infectious virus in large numbers of clinical specimens. Recently, Yong-He et al (1984) reported the use of EIA to detect several flaviviruses in cultured cells which were formalin-fixed prior to processing. Many rodent viruses do not induce morphologic changes in inoculated cell cultures, necessitating the use of immunochemical probes to detect viral antigens.…”

Section: Introductionmentioning

confidence: 99%

An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells

Smith

1985

Journal of Virological Methods

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An enzyme immunoassay was developed to identify and quantify infectious murine parvovirus. The assay was as sensitive as the fluorescent focus test for quantification of virus in clinical material and was less labor-intensive. Viral infectivity could be inactivated with formalin prior to performance of the enzyme immunoassay. Specificity of the reaction product was shown by neutralization of infectivity using a hyperimmune rabbit antiserum to murine parvovirus.

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Section: Introductionmentioning

confidence: 99%

An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells

Smith

1985

Journal of Virological Methods

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“…As an alternative to the PRA, we used an enzyme-linked immunosorbent assay (ELISA) for evaluating, by color reduction, antiviral agents active against VZV. The ELISA was performed directly on fixed infected monolayers in microtiter plates, a technique previously used to type or identify viruses and to measure antibody or interferon activity (1,8,10,14).…”

mentioning

confidence: 99%

Use of an enzyme-linked immunosorbent assay performed directly on fixed infected cell monolayers for evaluating drugs against varicella-zoster virus

Berkowitz¹,

Levin²

1985

Antimicrob Agents Chemother

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An in situ enzyme-linked immunosorbent assay (ELISA), performed directly on fixed varicella-zoster virus-infected monolayers, was used to quantitate viral antigen, and by color reduction it was used to evaluate the activity of drugs against varicella-zoster virus. Color production in the ELISA (optical density) was directly related to the dose of input virus. Antigen representing 5 to 10 plaques could be detected 3 days after infection. The ELISA was specific and reproducible, as shown by absorption and repeat experiments, respectively. A color-reduction test by ELISA was compared with the conventional plaque-reduction assay for its ability to measure the antiviral activity of acyclovir, bromovinyldeoxyuridine, trifluorothymidine, and vidarabine against four strains of varicella-zoster virus. In all cases but one the 50% inhibitory doses were lower when measured by ELISA than by the plaque-reduction assay. This in situ ELISA color reduction method had the following advantages over the conventional plaque-reduction assay: (i) the endpoint was an objective measurement; (ii) there was less well-to-well variation; (iii) the assay was sensitive to changes in plaque size as well as plaque number; (iv) it was less labor intensive.

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Arbovirus Detection in Vectors

Paradkar

Karl

2021

Genetically Modified and Other Innovative Vector Control Technologies

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A simplified new method for the identification of arboviruses: Virus detection using enzyme immunoassay on cultured cells

Cited by 12 publications

References 7 publications

An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells

An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells

Use of an enzyme-linked immunosorbent assay performed directly on fixed infected cell monolayers for evaluating drugs against varicella-zoster virus

Arbovirus Detection in Vectors

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