A simplified objective method for quantification of peste des petits ruminants virus or neutralizing antibody

Raj, G. Dhinakar; Nachimuthu, K.; Nainar, A. Mahalinga

doi:10.1016/s0166-0934(00)00206-8

Cited by 23 publications

(17 citation statements)

References 22 publications

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“…Animals (ca. 1 year of age) for the experiment were initially tested to be negative for the presence of PPRV antibody by competitive ELISA (Singh et al, 2004b) and serum neutralization test (SNT; Dhinakar Raj et al, 2000). The animals were also found negative for PPRV antigen in nasal, ocular, buccal, and rectal swabs by sandwich ELISA (Singh et al, 2004c).…”

Section: Methodsmentioning

confidence: 99%

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

et al. 2017

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Peste des petits ruminants (PPR) is one of the highly contagious viral disease, characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, primarily affecting sheep and goats. Reports suggested variable host response in goats and sheep and this host response vis-a-vis the expression of microRNAs (miRNAs) has not been investigated. Here, miRNAs were sequenced and proteomics data were generated to identify the role of differentially expressed miRNA (DEmiRNA) in PPR virus (PPRV) infected lung and spleen tissues of sheep and goats. In lungs, 67 and 37 DEmiRNAs have been identified in goats and sheep, respectively. Similarly, in spleen, 50 and 56 DEmiRNAs were identified in goats and sheep, respectively. A total of 20 and 11 miRNAs were found to be common differentially expressed in both the species in PPRV infected spleen and lung, respectively. Six DEmiRNAs—miR-21-3p, miR-1246, miR-27a-5p, miR-760-3p, miR-320a, and miR-363 were selected based on their role in viral infections, apoptosis, and fold change. The target prediction analysis of these six selected DEmiRNAs from the proteome data generated, revealed involvement of more number of genes in lung and spleen of goats than in sheep. On gene ontology analysis of host target genes these DEmiRNAs were found to regulate several immune response signaling pathways. It was observed that the pathways viz. T cell receptor signaling, Rap1 signaling, Toll-like receptor signaling, and B cell receptor signaling governed by DEmiRNAs were more perturbed in goats than in sheep. The data suggests that PPRV-induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis in the lung and spleen of sheep by downregulating several immune response genes. The study gives an important insight into the molecular pathogenesis of PPR by identifying that the PPRV—Izatnagar/94 isolate elicits a strong host response in goats than in sheep.

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Section: Methodsmentioning

confidence: 99%

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

et al. 2017

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“…Currently, the OIE recommend the use of the competitive PPRVspecific anti-H monoclonal based ELISA (cH-ELISA) (Anderson & McKay, 1994) and virus neutralization tests (FAO, 1996). However, several alternatives exist (Choi et al, 2005;Libeau et al, 1995) including the indirect N ELISA (Ismail et al, 1995), immunofiltration (Dhinakar Raj et al, 2008), a novel sandwich ELISA (Saravanan et al, 2008), haemagglutination tests (Dhinakar Raj et al, 2000;Ezeibe et al, 2008) and latex agglutination tests (Keerti et al, 2009).…”

Section: Pprv: Diagnosis Prevention and Future Controlmentioning

confidence: 99%

Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control

Banyard¹,

Parida

Batten

et al. 2010

Journal of General Virology

384

420

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“…With the aim to be implemented as a simple test, an HI assay employing 0.5 % chicken RBC has been described to correlate well with the VNT (Dhinakar et al 2000). The method measures the whole-serum immunoglobulins and as for the VNT and c-ELISA is able to test sera from any species, making it useful for the surveillance studies.…”

Section: Hemagglutination Inhibition (Hi)mentioning

confidence: 99%

Current Advances in Serological Diagnosis of Peste des Petits Ruminants Virus

Libeau

2014

Peste Des Petits Ruminants Virus

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Peste des petits ruminants (PPR) is a contagious disease with potential for high economic impacts. Convalescent and vaccinated small ruminants develop a strong and lifelong immunity and are fully protected against re-infection. Consequently, the presence of antibodies, whether they are against a wild virus or a vaccine, is a marker of the host immune protection. Thus, specific antibodies cannot be missed with classical and validated diagnosis assays, such as conventional enzyme-linked immunosorbent assay (ELISA), an alternative testing method to the conventional virus neutralization test (VNT), allowing to monitor vaccination or locate disease outbreaks. Recent insights into the virions' protein composition, development of expression systems, recombinant vectors to express protective antigens, have contributed greatly to update our current knowledge about diagnostics. In the light of this progress, the chapter focuses on PPRV serological tests with particular attention to diagnostic antigen targets and refinement of assays for accurate serodiagnosis of PPRV.

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A simplified objective method for quantification of peste des petits ruminants virus or neutralizing antibody

Cited by 23 publications

References 22 publications

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

Modulation of Host miRNAs Transcriptome in Lung and Spleen of Peste des Petits Ruminants Virus Infected Sheep and Goats

Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control

Current Advances in Serological Diagnosis of Peste des Petits Ruminants Virus

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