A Simplified Radioimmunoassay of Human Gravid Plasma Estriol

Den, K.; Fujii, Kiyoshi; Yoshida, Tsuyoshi; Takagi, Shigetaka

doi:10.1507/endocrj1954.20.315

Cited by 21 publications

(1 citation statement)

References 9 publications

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“…Most published methods for the determination of unconjugated oestriol depend upon an extraction of the plasma sample with an organic solvent, usually diethyl ether (Touchstone et al, 1963;Den et al, 1973;Mathur et al, 1973;Katagiri et al, 1974Katagiri et al, , 1976De Hertogh et al, 1975;Goebelsmann, 1973). The requirement for sample extraction limits the practicality of a routine assay and attempts have been made to develop direct assays in which the primary reagents (antiserum and tracer) are sufficiently specific to avoid the need for the extraction step.…”

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confidence: 99%

Assays for blood oestriol in the assessment of fetal well-being: Is an extraction step necessary?

Perry¹,

Fattah

Cochrane

et al. 1984

Journal of Obstetrics and Gynaecology

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confidence: 99%

Assays for blood oestriol in the assessment of fetal well-being: Is an extraction step necessary?

Perry¹,

Fattah

Cochrane

et al. 1984

Journal of Obstetrics and Gynaecology

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Studies on the mass fragmentographic determination of plasma estriol

Adlercreutz

Nylander

Hunneman³

1974

Biol. Mass Spectrom.

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Abstract-Three mass fragmentatographic methods for the determination of unconjugated estriol in pregnancy plasma and unconjugated and conjugated estriol in plasma of nonpregnant women after estriol administration were developed and tested as to their reliability and practicability. The methods were found to fulfii appropriate reliability criteria especially with regard to specificity. Unconjugated estriol could be assayed without prior chromatography in late pregnancy plasma, but a chromatographic step was needed for plasmas with a low estrioi titre, and both a methylation step and Chromatography is needed to achieve the required specificity if plasma conjugated estriol is determined or if assays are carried out following estrioi administration. Unconjugated estrioi in normal late pregnancy plasma was found in concentrations from 4.3 to 9.3 pg/L The highest value recorded, 16.3 pg/i, was found in a prediabetic subject with mild hypertension, who delivered a child weighing 4150 g by Cesarean section in the 39th week. Low values were found in severe hypertension and in toxemia, and in general the results from the pathological material investigated seemed to correlate well with the clinical findings. However, only a few samples (five to ten) can be processed so rapidly that the resuits can be obtained the same day, which in addition to the expensive and complicated instrumentation limits the usefulness of the methods in routine clinical chemistry.

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