A Simultaneous Assay for T-CelIs and B-Cells Using Immunobeads

Abstract

A comparison was made between a new, commercially available light microscope assay for T- and B-lymphocyte enumeration and existing procedures. The Quantigen assay employs two color-coded Immunobeads; one Immunobead binds to and labels T-cells, while the second labels B-cells. Phagocytic cells contaminating lymphocyte preparations ingest both types of beads. A high degree of correlation was found between this simultaneous assay for T- and B-cells and the E-rosette method for T-cells and FITC-labeling of B-cell surface immunoglobulin. The results of a comparison of the Quantigen assay to flow cytometric analyses are also given. Data on chronic lymphocytic leukemia samples are presented, demonstrating that these cells are double-markers with the Quantigen assay because of the use of monoclonal antibody T101, which recognizes T-cells and B-CLL cells.

In vivo protein synthesis of circulating human T lymphocytes does not respond to a cortisol challenge within 24 h

“…The rosette method is a well‐established technique. A comparison between E‐rosetting and Immunobead assay shows a high correlation between the two methods (13). In our hands the purity of the T cell population determined by flow cytometry is 90–95%.…”

A Comparative Analysis of Immunorestoration and Recovery with Conventional and Immunotherapeutic Protocols in Canine Generalized Demodicosis: A Newer Insight of Immunotherapeutic Efficacy of T11TS

“…The mixture was then centrifuged for 5 min at 200 g and was kept at 4°C overnight. After 18 hours, the pellet was gently resuspended after addition of 1% glutaraldehyde and lymphocytes with 3 or more SRBC constellation were counted per 200 lymphocytes under a light microscope and results were expressed as rosette percent (Ardaur and Newsholme, 1983; Barran and Parker, 1985;Parker, 1976;Plunkett et al, 1987).…”