A Single Amino Acid Change in the Response Regulator PhoP, Acquired during Yersinia pestis Evolution, Affects PhoP Target Gene Transcription and Polymyxin B Susceptibility

Abstract

, the causative agent of plague, evolved from the closely related pathogen During its emergence, is believed to have acquired its unique pathogenic characteristics through numerous gene gains/losses, genomic rearrangements, and single nucleotide polymorphism (SNP) changes. One such SNP creates a single amino acid variation in the DNA binding domain of PhoP, the response regulator in the PhoP/PhoQ two-component system. and the basal human-avirulent strains of harbor glycines at position 215 of PhoP, whereas the modern human-virulent strains (e.g., KIM and CO92) harbor serines at this residue. Since PhoP plays multiple roles in the adaptation of to stressful host conditions, we tested whether this amino acid substitution affects PhoP activity or the ability of to survive in host environments. Compared to the parental KIM6+ strain carrying the modern allele of (), a derivative carrying the basal allele () exhibited slightly defective growth under a low-Mg condition and decreased transcription of a PhoP target gene, , as well as an ∼8-fold increase in the susceptibility to the antimicrobial peptide polymyxin B. The strain showed no apparent defect in flea colonization, although a -null mutant showed decreased flea infectivity in competition experiments. Our results suggest that the amino acid variation at position 215 of PhoP causes subtle changes in the PhoP activity and raise the possibility that the change in this residue have contributed to the evolution of increased virulence in acquired a single nucleotide polymorphism (SNP) in when the highly human-virulent strains diverged from less virulent basal strains, resulting in an amino acid substitution in the DNA binding domain of the PhoP response regulator. We show that carrying the modern allele has an increased ability to induce the PhoP-regulated gene and resist antimicrobial peptides compared to an isogenic strain carrying the basal allele. Given the important roles PhoP plays in host adaptation, the results raise an intriguing possibility that this amino acid substitution contributed to the evolution of increased virulence in Additionally, we present the first evidence that confers a survival fitness advantage to inside the flea midgut.

To block or not to block: The adaptive manipulation of plague transmission

“…; Vadyvaloo and Hinz ; Fukuto et al. ). These studies revealed that fitness costs are associated with mutations in several genes involved in flea‐borne transmission ( hfq (Rempe et al.…”

“…), rovM (Vadyvaloo and Hinz ), and phoP (Fukuto et al. )). Unfortunately, experiments following the competition taking place between hms variants in the flea remain to be carried out.…”

“…Recent experimental studies have explored the outcome of competition between different strains of Y. pestis in fleas (Rempe et al 2012;Spinner et al 2013;Vadyvaloo and Hinz 2015;Fukuto et al 2018). These studies revealed that fitness costs are associated with mutations in several genes involved in flea-borne transmission (hfq (Rempe et al 2012), rovM (Vadyvaloo and Hinz 2015), and phoP (Fukuto et al 2018)). Unfortunately, experiments following the competition taking place between hms variants in the flea remain to be carried out.…”

To block or not to block: the adaptive manipulation of plague transmission

“…In particular, conditions (5) and (6) indicate that this mechanism is likely to promote the evolution of blockage. Recent experimental studies have explored the outcome of competition between different strains of Y. pestis in fleas (33–36). Unfortunately, experiments following the competition taking place between hms variants in the flea remain to be carried out.…”

The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis During Planktonic Growth and Growth in Biofilms

“…The small RNA chaperone, Hfq, is required for full production of Crp in Y. pestis, but not in Y. pseudotuberculosis (6,31). The PhoP response regulator is an activator of crp expression in one strain of Y. pestis (21), but variation in the DNAbinding domain of PhoP between Yersinia strains alters transcription of its target genes (46). In addition, these differences in regulation of the crp gene and the extent to what genes are regulated by Crp likely result from changes in the promoter region of genes and reflect adaptations to the different environments Y. pestis and Y. pseudotuberculosis inhabit.…”