A single amino acid change can alter the specificity of the multi-allelic wheat stem rust resistance locus SR9

Zhang, Jianping; Nirmala, Jayaveeramuthu; Chen, Shisheng; Jost, Matthias; Steuernagel, Burkhard; Karafiátová, Miroslava; Hewitt, Tim; Li, Hongna; Edae, Erena A.; Sharma, Keshav; Hoxha, Sami; Bhatt, Dhara; Antoniou-Kourounioti, Rea L; Dodds, Peter N.; Wulff, Brande B. H.; Doležel, Jaroslav; Ayliffe, Michael; Hiebert, Colin W.; McIntosh, R. A.; Dubcovsky, Jorge; Zhang, Peng; Rouse, Matthew N.; Lagudah, Evans

doi:10.21203/rs.3.rs-2208386/v1

Cited by 4 publications

(4 citation statements)

References 59 publications

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“…The candidate gene region includes 49 annotated high-con dence genes in Chinese Spring (TraesCS7A01G110400-TraesCS7A01G115200, Supplementary Table 6). The functional annotation of these genes revealed six typical NLR genes (Supplementary Table 6), a gene class frequently associated with disease resistance in plants [32][33][34][35][36][37] .…”

Section: Resultsmentioning

confidence: 99%

Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Hua

Zhao

et al. 2023

Preprint

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Leaf rust, caused by Puccinia triticinaEriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broad-spectrum leaf rust resistance gene introgressed into wheat fromAegilops speltoides. The Lr47 gene encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. New Lr47 introgression lines with no or reduced linkage dragwere generated using the ph1b mutation, and a diagnostic molecular marker for Lr47 was developed. The CC domain of the Lr47 protein was not able to induce cell death, nor did it have self-protein interaction. The cloning of Lr47 expands the number of Pt-resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.

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Section: Resultsmentioning

confidence: 99%

Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Hua

Zhao

et al. 2023

Preprint

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“…Our in-silico structural modelling and analysis did not show an association between the full-length structures or diverse N terminal structures of SR6, SR13, SR21 and temperature sensitivity of resistance. Whereas Sr13 encodes a classic CC-NB-LRR, Sr21 and the SR9 allelic series encode NLR proteins with a unique N terminus comprised of ve helices bundle 49 , and Sr6 falls into the zinc nger BED domain containing R protein encoding gene subset. Interestingly, we observed that all α3 helices from these proteins were the closest unit to their respective LRR domains.…”

Section: Discussionmentioning

confidence: 99%

Divergent molecular pathways govern temperature-dependent wheat stem rust resistance genes Sr6, Sr13 and Sr21

Zhang,

Hewitt,

Sharma

et al. 2024

Preprint

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The wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), has caused devastating crop losses worldwide. Several stem rust resistance (Sr) genes display temperature-dependent immune responses. Sr6-mediated resistance is enhanced at lower temperatures whereas Sr13 and Sr21 resistances are enhanced at higher temperatures. Here we report cloning of Sr6 by mutagenesis and resistance gene enrichment and sequencing (MutRenSeq), showing it to encode an NLR protein with an integrated BED domain. Sr6 temperature sensitivity was also transferred to wheat plants transformed with the Sr6 transgene. Differential gene expression analysis using near-isogenic wheat lines inoculated with Pgt at varying temperatures revealed that genes upregulated in the low-temperature-effective Sr6 response differed significantly from those upregulated in the high-temperature-effective responses associated with Sr13 and Sr21. Understanding the molecular mechanisms and pathways involved in temperature sensitivity can inform future strategies for deployment and engineering of genetic resistance in response to a changing climate.

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“…High-resolution mapping of SrTm4 reveals an inversion linked to SrTm4 resistance Most plant disease resistance genes are dominant or partially dominant, but the presence of recessive R genes has also been well documented. However, the nature of wheat stem rust recessive genes remains largely unknown, since all 18 Sr genes cloned so far in wheat and its wild relatives are all dominant or partially dominant (Zhang et al 2022). The recessive nature of SrTm4 and the mesothetic resistant infection types may be indicative of a different mechanism, providing an added incentive to clone this gene.…”

Section: Discussionmentioning

confidence: 99%

High-resolution mapping of SrTm4, a recessive resistance gene to wheat stem rust

Li¹,

Luo²,

Zhang

et al. 2023

Preprint

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Race Ug99 of Puccinia graminis f. sp. tritici (Pgt), the causal agent of wheat stem (or black) rust is one of the most serious threats to global wheat production. The identification, mapping, and deployment of effective stem rust resistance (Sr) genes are critical to reduce this threat. In this study, we generated SrTm4 monogenic lines and found that this gene confers resistance to North American and Chinse Pgt races. Using a large mapping population (9,522 gametes), we mapped SrTm4 within a 0.06 cM interval flanked by marker loci CS4211 and 130K1519, which corresponds to a 1.0-Mb region in the Chinese Spring reference genome v2.1. Physical map of the SrTm4 region was constructed with 11 overlapping BACs from the resistant T. monococcum PI 306540. The comparison of the 754-kb physical map with the genomic sequence of Chinese Spring and the discontinuous BAC sequence of DV92 revealed a 593-kb chromosomal inversion in PI 306540. Within the candidate region, we identified an L-type lectin-domain containing receptor kinase (LLK1), which was disrupted by the proximal inversion breakpoint, as a potential candidate gene. Two diagnostic dominant markers were developed to detect the inversion breakpoints. In a survey of T. monococcum accessions, we identified only 10 domesticated T. monococcum subsp. monococcum genotypes, mainly from the Balkans, carrying the inversion and showing similar mesothetic resistant infection types against Pgt races. The high-density map and the tightly linked molecular markers developed in this study are useful tools to accelerate the deployment of SrTm4-mediated resistance in wheat breeding programs.

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A single amino acid change can alter the specificity of the multi-allelic wheat stem rust resistance locus SR9

Cited by 4 publications

References 59 publications

Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Divergent molecular pathways govern temperature-dependent wheat stem rust resistance genes Sr6, Sr13 and Sr21

High-resolution mapping of SrTm4, a recessive resistance gene to wheat stem rust

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