A single bout of brisk walking increases basal very low-density lipoprotein triacylglycerol clearance in young men

Yiannis, Tsekouras E.; Amalia, Yanni E.; Bougatsas, Dimitrios; Kavouras, Stavros A.; Sidossis, Labros S.

doi:10.1016/j.metabol.2007.03.012

Cited by 37 publications

(18 citation statements)

References 44 publications

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“…Compared with a control day of isocaloric feeding and rest, we found that exercise decreased fasting plasma VLDL-TG concentration by ~30%, owing to a 17% reduction in hepatic VLDL-TG secretion rate and a 22% increase in the plasma clearance rate of VLDL-TG, whereas diet had no effect on VLDL-TG concentration and kinetics. The findings from our study indicate that (i) exercise-induced hypotriglyceridemia in women manifests through a different mechanism (increased clearance and decreased secretion of VLDL-TG) than that described previously in men (increased clearance of VLDL-TG only) (26, 40), and (ii) exercise affects TG homeostasis by eliciting changes in VLDL-TG kinetics that are independent of negative energy balance and specific to muscular contraction.…”

Section: Discussioncontrasting

confidence: 43%

“…A 3-ml aliquot of plasma was transferred into plastic culture tubes and kept in the refrigerator for immediate isolation of VLDL, and the remaining plasma samples were stored at -80°C until analyses. The VLDL fraction was prepared by density-gradient ultracentrifugation, VLDL-TG were isolated by thin-layer chromatography, hydrolyzed, and VLDL-TG-bound glycerol was derivatized with heptafluorobutyric anhydride, as previously described (25, 40). The tracer to tracee ratio (TTR) of glycerol in VLDL-TG was measured by gas chromatography-mass spectrometry (MSD 5973 system; Hewlett-Packard, Palo Alto, CA) by selectively monitoring the ions at mass-to-charge ratios 467 and 472 (25, 40).…”

Section: Methodsmentioning

confidence: 99%

“…Exercise-induced hypotriglyceridemia manifests above a certain threshold of exercise energy expenditure (7), independent of duration and intensity (41), and plateaus with progressively more exercise (9). In a series of studies in healthy non-obese men, we have shown that a single bout of aerobic exercise reduces fasting plasma TG concentrations the next day by increasing the clearance rate of VLDL-TG from the circulation, without affecting VLDL-TG secretion rate from the liver (23, 25, 26, 40). The exercise-induced increase in VLDL-TG plasma clearance rate also requires a certain energy expenditure threshold and plateaus thereafter (20).…”

Section: Introductionmentioning

confidence: 99%

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Acute Effects of Exercise and Calorie Restriction on Triglyceride Metabolism in Women

Bellou¹,

Siopi²,

Galani³

et al. 2013

Medicine &Amp; Science in Sports &Amp; Exercise

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The mechanisms by which exercise reduces fasting plasma triglyceride (TG) concentrations in women and the effect of negative energy balance independent of muscular contraction are not known. Purpose The aim of this study was to evaluate the effects of equivalent energy deficits induced by exercise or calorie restriction on basal very low-density lipoprotein (VLDL) TG metabolism in women. Methods Eleven healthy women (age: 23.5±2.7 years, BMI: 21.6±1.4 kg/m2) underwent a stable isotopically labeled tracer infusion study to determine basal VLDL-TG kinetics after performing, in random order, three experimental trials on the previous day: i) a single exercise bout (brisk walking at 60% of peak oxygen consumption for 123±18 min, with a net energy expenditure of 2.06±0.39 MJ (~500 kcal)), ii) dietary energy restriction of 2.10±0.41 MJ, and iii) a control day of isocaloric feeding and rest (zero energy balance). Results Fasting plasma VLDL-TG concentration was ~30% lower after the exercise trial compared to the control trial (P<0.001), whereas no significant change was detected after the calorie restriction trial (P=0.297 vs control). Relative to the control condition, exercise increased the plasma clearance rate of VLDL-TG by 22% (P=0.001) and reduced hepatic VLDL-TG secretion rate by ~17% (P=0.042), whereas hypocaloric diet had no effect on VLDL-TG kinetics (P>0.2). Conclusion (i) Exercise-induced hypotriglyceridemia in women manifests through a different mechanism (increased clearance and decreased secretion of VLDL-TG) than that previously described in men (increased clearance of VLDL-TG only), and (ii) exercise affects TG homeostasis by eliciting changes in VLDL-TG kinetics that cannot be reproduced by an equivalent diet-induced energy deficit, indicating that these changes are independent of the exercise-induced negative energy balance but instead are specific to muscular contraction.

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Section: Discussioncontrasting

confidence: 43%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acute Effects of Exercise and Calorie Restriction on Triglyceride Metabolism in Women

Bellou¹,

Siopi²,

Galani³

et al. 2013

Medicine &Amp; Science in Sports &Amp; Exercise

View full text Add to dashboard Cite

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“…Further support of this comes from the same lab where moderate intensity exercise had no effect on TG clearance, monitored after intralipid infusion [42]. On the other hand further study has shown that there was no effect of moderate intensity exercise on hepatic VLDL-TG secretion rate [43][44]. It seems then, that moderate intensity exercise may attenuate postprandial TG via an increased LPL activity (possibly above a threshold energy expenditure), with conflicting evidence as to whether decreased hepatic VLDL-TG secretions play a major role.…”

Section: Discussionmentioning

confidence: 98%

The Effect of High Intensity Interval Exercise on Postprandial Triacylglycerol and Leukocyte Activation – Monitored for 48h Post Exercise

et al. 2013

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Postprandial phenomenon are thought to contribute to atherogenesis alongside activation of the immune system. A single bout of high intensity interval exercise attenuates postprandial triacylglycerol (TG), although the longevity and mechanisms underlying this observation are unknown. The aims of this study were to determine whether this attenuation in postprandial TG remained 2 days after high intensity interval exercise, to monitor markers of leukocyte activation and investigate the underlying mechanisms. Eight young men each completed two three day trials. On day 1: subjects rested (Control) or performed 5 x 30 s maximal sprints (high intensity interval exercise). On day 2 and 3 subjects consumed high fat meals for breakfast and 3 h later for lunch. Blood samples were taken at various times and analysed for TG, glucose and TG-rich lipoprotein (TRL)-bound LPL-dependent TRL-TG hydrolysis (LTTH). Flow cytometry was used to evaluate granulocyte, monocyte and lymphocyte CD11b and CD36 expression. On day 2 after high intensity interval exercise TG area under the curve was lower (P<0.05) (7.46±1.53 mmol/l/7h) compared to the control trial (9.47±3.04 mmol/l/7h) with no differences during day 3 of the trial. LTTH activity was higher (P<0.05) after high intensity interval exercise, at 2 hours of day 2, compared to control. Granulocyte, monocyte and lymphocyte CD11b expression increased with time over day 2 and 3 of the study (P<0.0001). Lymphocyte and monocyte CD36 expression decreased with time over day 2 and 3 (P<0.05). There were no differences between trials in CD11b and CD36 expression on any leukocytes. A single session of high intensity interval exercise attenuated postprandial TG on day 2 of the study, with this effect abolished by day 3.The reduction in postprandial TG was associated with an increase in LTTH. High intensity interval exercise had no effect on postprandial responses of CD11b or CD36.

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“…A 3-ml aliquot of plasma was transferred into plastic culture tubes and kept in the refrigerator for immediate isolation of VLDL, and the remaining plasma samples were stored at −80°C until analyses. The VLDL fraction was prepared by density-gradient ultracentrifugation, VLDL-TG were isolated by thin-layer chromatography, hydrolyzed, and VLDL-TG-bound glycerol was derivatized with heptafluorobutyric anhydride, as previously described [21], [22]. The tracer to tracee ratio (TTR) of glycerol in VLDL-TG was measured by gas chromatography-mass spectrometry (MSD 5973 system; Hewlett-Packard, Palo Alto, CA) by selectively monitoring the ions at mass-to-charge ratios 467 and 472 [21], [22].…”

Section: Methodsmentioning

confidence: 99%

Effect of Acute Negative and Positive Energy Balance on Basal Very-Low Density Lipoprotein Triglyceride Metabolism in Women

et al. 2013

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BackgroundAcute reduction in dietary energy intake reduces very low-density lipoprotein triglyceride (VLDL-TG) concentration. Although chronic dietary energy surplus and obesity are associated with hypertriglyceridemia, the effect of acute overfeeding on VLDL-TG metabolism is not known.ObjectiveThe aim of the present study was to investigate the effects of acute negative and positive energy balance on VLDL-TG metabolism in healthy women.DesignTen healthy women (age: 22.0±2.9 years, BMI: 21.2±1.3 kg/m2) underwent a stable isotopically labeled tracer infusion study to determine basal VLDL-TG kinetics after performing, in random order, three experimental trials on the previous day: i) isocaloric feeding (control) ii) hypocaloric feeding with a dietary energy restriction of 2.89±0.42 MJ and iii) hypercaloric feeding with a dietary energy surplus of 2.91±0.32 MJ. The three diets had the same macronutrient composition.ResultsFasting plasma VLDL-TG concentrations decreased by ∼26% after hypocaloric feeding relative to the control trial (P = 0.037), owing to decreased hepatic VLDL-TG secretion rate (by 21%, P = 0.023) and increased VLDL-TG plasma clearance rate (by ∼12%, P = 0.016). Hypercaloric feeding increased plasma glucose concentration (P = 0.042) but had no effect on VLDL-TG concentration and kinetics compared to the control trial.ConclusionAcute dietary energy deficit (∼3MJ) leads to hypotriglyceridemia via a combination of decreased hepatic VLDL-TG secretion and increased VLDL-TG clearance. On the other hand, acute dietary energy surplus (∼3MJ) does not affect basal VLDL-TG metabolism but disrupts glucose homeostasis in healthy women.

show abstract

A single bout of brisk walking increases basal very low-density lipoprotein triacylglycerol clearance in young men

Cited by 37 publications

References 44 publications

Acute Effects of Exercise and Calorie Restriction on Triglyceride Metabolism in Women

Acute Effects of Exercise and Calorie Restriction on Triglyceride Metabolism in Women

The Effect of High Intensity Interval Exercise on Postprandial Triacylglycerol and Leukocyte Activation – Monitored for 48h Post Exercise

Effect of Acute Negative and Positive Energy Balance on Basal Very-Low Density Lipoprotein Triglyceride Metabolism in Women

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