A single-cell atlas of human teeth

Pagella, Pierfrancesco; Roditi, Laura De Vargas; Stadlinger, Bernd; Moor, Andreas E.; Mitsiadis, Thimios A.

doi:10.1016/j.isci.2021.102405

Cited by 75 publications

(101 citation statements)

References 71 publications

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“…Recently, we performed a thorough single cell RNA sequencing analysis of the human dental pulp and periodontium. 37 We here interrogated this dataset to determine the expression of NOTCH receptors and ligands in dental pulp and periodontal MSCs (Figures 1 and 2 ). In the dental pulp, MSCs were characterized by the higher expression of NOTCH3 (log‐fold change of 1.24 and adjusted P value <.001, compared to other cell types in the pulp) (Figure 1A ).…”

Section: Resultsmentioning

confidence: 99%

“…NOTCH3 + MSCs also expressed the dental MSCs markers THY1 , MYH11 , and ACTA2 (Figure 1A ). 20 , 37 , 39 NOTCH3 ‐expressing MSCs were mainly localized around the vessels, where the perivascular niches are formed 21 , 22 (Figure 1C‐G ). Double staining against Notch3 and the endothelial cell marker CD31, followed by cell surface analysis, showed that the Notch3‐positive MSCs (pericytes) are in direct contact with the CD31‐positive endothelial cells (Figure 1H,I ).…”

Section: Resultsmentioning

confidence: 99%

“…A, UMAP visualization of color‐coded clustering of the dental pulp (n > 32 000 cells). 37 B, Hematoxylin‐eosin staining showing the structure of the dental pulp. C‐E, Immunohistochemistry against NOTCH3 (red color) in the human dental pulp showing the localization of NOTCH3 in pericytes.…”

Section: Resultsmentioning

confidence: 99%

“…A, UMAP visualization of color‐coded clustering of the periodontium (n > 2800 cells). 37 B, Hematoxylin‐eosin staining showing the structure of the periodontium. C,D, Immunofluorescent staining showing localization of NOTCH3 + (green color) and ACTA2 + (red color) mesenchymal stem cells (MSCs).…”

Section: Resultsmentioning

confidence: 99%

“…All code is publicly available at: https://github.com/TheMoorLab/Tooth . Single cell RNA sequencing data are described in detail in Reference 37 .…”

Section: Methodsmentioning

confidence: 99%

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Notch Signaling in the Dynamics of Perivascular Stem Cells and their Niches

Pagella

Roditi

Stadlinger

et al. 2021

Stem Cells Translational Medicine

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The Notch signaling pathway is a fundamental regulator of cell fate determination in homeostasis and regeneration. In this work, we aimed to determine how Notch signaling mediates the interactions between perivascular stem cells and their niches in human dental mesenchymal tissues, both in homeostatic and regenerative conditions.By single cell RNA sequencing analysis, we showed that perivascular cells across the dental pulp and periodontal human tissues all express NOTCH3, and that these cells are important for the response to traumatic injuries in vivo in a transgenic mouse model. We further showed that the behavior of perivascular NOTCH3-expressing stem cells could be modulated by cellular and molecular cues deriving from their microenvironments. Taken together, the present studies, reinforced by single-cell analysis, reveal the pivotal importance of Notch signaling in the crosstalk between perivascular stem cells and their niches in tissue homeostasis and regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…All code is publicly available at: https://github.com/TheMoorLab/Tooth . Single cell RNA sequencing data are described in detail in Reference 37 .…”

Section: Methodsmentioning

confidence: 99%

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Notch Signaling in the Dynamics of Perivascular Stem Cells and their Niches

Pagella

Roditi

Stadlinger

et al. 2021

Stem Cells Translational Medicine

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Mapping Cell Atlases at the Single‐Cell Level

Ye,

Wang,

et al. 2023

Advanced Science

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Recent advancements in single‐cell technologies have led to rapid developments in the construction of cell atlases. These atlases have the potential to provide detailed information about every cell type in different organisms, enabling the characterization of cellular diversity at the single‐cell level. Global efforts in developing comprehensive cell atlases have profound implications for both basic research and clinical applications. This review provides a broad overview of the cellular diversity and dynamics across various biological systems. In addition, the incorporation of machine learning techniques into cell atlas analyses opens up exciting prospects for the field of integrative biology.

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Diverse stem cells for periodontal tissue formation and regeneration

English

Ono

2022

Genesis

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Summary The periodontium is comprised of multiple units of mineralized and nonmineralized tissues including the cementum on the root surface, the alveolar bone, periodontal ligament (PDL), and the gingiva. PDL contains a variety of cell populations including mesenchymal stem/progenitor cells (MSCs) termed PDLSCs, which contribute to periodontal regeneration. Recent studies utilizing mouse genetic models shed light on the identities of these mesenchymal progenitors in their native environment, particularly regarding how they contribute to homeostasis and repair of the periodontium. The current concept is that mesenchymal progenitors in the PDL are localized to the perivascular niche. Single‐cell RNA sequencing (scRNA‐seq) analyses reveal heterogeneity and cell‐type specific markers of cells in the periodontium, as well as their developmental relationship with precursor cells in the dental follicle. The characteristics of PDLSCs and their diversity in vivo are now beginning to be unraveled thanks to insights from mouse genetic models and scRNA‐seq analyses, which aid to uncover the fundamental properties of stem cells in the human PDL. The new knowledge will be highly important for developing more effective stem cell‐based regenerative therapies to repair periodontal tissues in the future.

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A single-cell atlas of human teeth

Cited by 75 publications

References 71 publications

Notch Signaling in the Dynamics of Perivascular Stem Cells and their Niches

Notch Signaling in the Dynamics of Perivascular Stem Cells and their Niches

Mapping Cell Atlases at the Single‐Cell Level

Diverse stem cells for periodontal tissue formation and regeneration

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