A single‐cell transcriptomic atlas characterizes cell types and their molecular features in yak ovarian cortex

Pei, Jie; Xiong, Lin; Guo, Shaoke; Wang, Xingdong; Bao, Pengjia; Wu, Xiaoyun; Yan, Ping; Guo, Xian

doi:10.1096/fj.202201176rr

Cited by 8 publications

(5 citation statements)

References 71 publications

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“…This is consistent with the findings of Fan et al [73]. In addition, with the popularity of single-cell studies, cellular characteristics and intercellular communication during yak follicle development have been characterized [110, 111].…”

Section: The Findings Of Single-cell Studies In Physiology Of Follicu...supporting

confidence: 90%

Follicular development and ovary aging: single-cell studies

Zhao

Wang

Yang

2023

Biology of Reproduction

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Follicular development is a critical process in reproductive biology that determines the number of oocytes and interacts with various cells within the follicle (such as oocytes, granulosa cells, cumulus cells and theca cells, etc.), and plays a vital role in fertility and reproductive health due to the dogma of a limited number of oogonia. Dysregulation of follicular development can lead to infertility problems and other reproductive disorders. To explore the physiological and pathological mechanisms of follicular development, immunology-based methods, microarrays, and next-generation sequencing have traditionally been used for characterization at the tissue level. However, with the proliferation of single-cell sequencing techniques, research has uncovered unique molecular mechanisms in individual cells that have been masked by previous holistic analyses. In this review, we briefly summarize the achievements and limitations of traditional methods in the study of follicular development. Simultaneously, we focus on how to understand the physiological process of follicular development at the single-cell level and reveal the relevant mechanisms leading to the pathology of follicular development and intervention targets. Moreover, we also summarize the limitations and application prospects of single cell sequencing in follicular development research.

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Section: The Findings Of Single-cell Studies In Physiology Of Follicu...supporting

confidence: 90%

Follicular development and ovary aging: single-cell studies

Zhao

Wang

Yang

2023

Biology of Reproduction

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“…Testicular tissue was sectioned, stained, and immunohistochemically verified using previously established methods [57,58]. The tissue was rinsed with running saline and then cut into small pieces with a size of about 5 mm 3 using a sterile scalpel.…”

Section: Histological Observation and Immunohistochemical Analysismentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals Atlas of Yak Testis Cells

Wang

Pei

Xiong

et al. 2023

IJMS

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Spermatogenesis is a complex process that involves proliferation and differentiation of diploid male germ cells into haploid flagellated sperm and requires intricate interactions between testicular somatic cells and germ cells. The cellular heterogeneity of this process presents a challenge in analyzing the different cell types at various developmental stages. Single-cell RNA sequencing (scRNA-seq) provides a useful tool for exploring cellular heterogeneity. In this study, we performed a comprehensive and unbiased single-cell transcriptomic study of spermatogenesis in sexually mature 4-year-old yak using 10× Genomics scRNA-seq. Our scRNA-seq analysis identified six somatic cell types and various germ cells, including spermatogonial stem cells, spermatogonia, early-spermatocytes, late-spermatocytes, and spermatids in yak testis. Pseudo-timing analysis showed that Leydig and myoid cells originated from common progenitor cells in yaks. Moreover, functional enrichment analysis demonstrated that the top expressed genes in yak testicular somatic cells were significantly enriched in the cAMP signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, and ECM receptor interactions. Throughout the spermatogenesis process, genes related to spermatogenesis, cell differentiation, DNA binding, and ATP binding were expressed. Using immunohistochemical techniques, we identified candidate marker genes for spermatogonial stem cells and Sertoli cells. Our research provides new insights into yak spermatogenesis and the development of various types of cells in the testis, and presents more reliable marker proteins for in vitro culture and identification of yak spermatogonial stem cells in the later stage.

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“…They also discovered that the yak oocytes regulated the other types of ovarian cells primarily via the interaction between the ligands FAM3, INHA, and JAG1 with their corresponding receptors. However, the endothelial, epithelial, and granulosa cells regulated the oocytes principally via the BMP family [27].…”

Section: Dynamic Transcripts and Protein Changesmentioning

confidence: 99%

Factors Influencing the Maturation and Developmental Competence of Yak (Bos grunniens) Oocytes In Vitro

Mo,

Ma,

Xiong

et al. 2023

Genes

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The yak (Bos grunniens) is a unique breed living on the Qinghai–Tibet Plateau and its surrounding areas, providing locals with a variety of vital means of living and production. However, the yak has poor sexual maturity and low fertility. High-quality mature oocytes are the basis of animal breeding technology. Recently, in vitro culturing of oocytes and embryo engineering technology have been applied to yak breeding. However, compared to those observed in vivo, the maturation rate and developmental capacity of in vitro oocytes are still low, which severely limits the application of in vitro fertilization and embryo production in yaks. This review summarizes the endogenous and exogenous factors affecting the in vitro maturation (IVM) and developmental ability of yak oocytes reported in recent years and provides a theoretical basis for obtaining high-quality oocytes for in vitro fertilization and embryo production in yaks.

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A single‐cell transcriptomic atlas characterizes cell types and their molecular features in yak ovarian cortex

Cited by 8 publications

References 71 publications

Follicular development and ovary aging: single-cell studies

Follicular development and ovary aging: single-cell studies

Single-Cell RNA Sequencing Reveals Atlas of Yak Testis Cells

Factors Influencing the Maturation and Developmental Competence of Yak (Bos grunniens) Oocytes In Vitro

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