A single extraction method for the determination of both norepinephrine and serotonin in brain

Mead, J. A. R.; Finger, Kenneth F.

doi:10.1016/0006-2952(61)90069-7

Cited by 139 publications

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“…Caudate nucleus and blood serum histamine were analysed fluorimetrically according to the method of Anton & Sayre (1968, 1969. Noradrenaline (NA) and 5-hydroxytryptamine (5-HT) homogenates were analysed fluorimetrically according to the method of Mead & Finger (1961). Serum blood glucose were analysed by the enzymatic-colorimetric method of Hugget & Nixon (1957).…”

Section: Methodsmentioning

confidence: 99%

Rat brain amines, blood histamine and glucose levels in relationship to circadian changes in sleep induced by pentobarbitone sodium

Friedman¹,

Walker²

1969

The Journal of Physiology

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1. The circadian patterns for onset and duration of sleep after the administration of a constant dose of pentobarbitone sodium have been measured in rats adapted to and maintained under a fixed illumination cycle. The relationship of these patterns to others for rectal temperature and motor activity as well as for noradrenaline (NA), 5‐hydroxytryptamine (5‐HT) and histamine in mid‐brain and caudate nucleus, and histamine and glucose in blood serum, has been examined. 2. The onset of sleep is longest and duration shortest during the phase of the illumination cycle when motor activity and rectal temperature are maximal. An inverse relationship for these parameters is found during the light phase of the illumination cycle. 3. In untreated rats, mid‐brain NA and histamine and caudate nucleus histamine levels are maximal and 5‐HT minimal during the dark phase of the illumination cycle. An inverse relationship for these parameters is found during the light phase of the illumination cycle. 4. Pentobarbitone sodium treatment significantly elevates mid‐brain NA and histamine and caudate nucleus histamine levels during the dark phase of the illumination cycle. Although 5‐HT levels are reduced over the entire circadian cycle this change is significant only during the light phase of the illumination cycle. 5. Pentobarbitone sodium treatment reverses the circadian pattern for body temperature, producing a mirror image of the control pattern. 6. The circadian pattern for blood serum histamine levels differs from the C.N.S. pattern for histamine. Peak levels occur at the end of the light phase of the illumination cycle. After pentobarbitone sodium these levels are reduced, although the circadian pattern is similar to the control. 7. Circadian blood glucose patterns have bimodal peaks; a primary peak at the end of the dark phase, a secondary peak at the end of the light phase of the illumination cycle. Pentobarbitone sodium did not significantly alter the pattern. The relationship between this pattern and C.N.S. and peripheral histamine and catechol amines is discussed.

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Section: Methodsmentioning

confidence: 99%

Rat brain amines, blood histamine and glucose levels in relationship to circadian changes in sleep induced by pentobarbitone sodium

Friedman¹,

Walker²

1969

The Journal of Physiology

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“…The mid-brain and both caudate nuclei were removed within 2 min after decapitation, weighed and homogenized either in cold 0 01N-HCI or cold 0-4N perchloric acid. Homogenates were analysed fluorimetrically for noradrenaline and 5-HT (Mead & Finger, 1961) and for histamine (Shore, Burkhalter & Cohn, 1959). These analyses were made within 4 hr of the animals being killed.…”

Section: Methodsmentioning

confidence: 99%

Circadian rhythms in rat mid‐brain and caudate nucleus biogenic amine levels

Friedman¹,

Walker²

1968

The Journal of Physiology

156

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SUMMARY1. The circadian rhythms of rectal temperature and biogenic amine levels in mid-brain and caudate nucleus have been measured in normal and adrenalectomized rats adapted to and maintained under fixed illumination cycle.2. Rectal temperature reaches a peak value between 24.00 hr and 06.00 hr during the dark phase of the illumination cycle at a time when motor activity is maximal. In adrenalectomized rats, the pattern is similar but the peak is significantly lower.3. Highest histamine levels in the caudate nucleus and mid-brain of normal and adrenalectomized rats are found at the time when body temperature and motor activity is maximal.4. Similarly, caudate nucleus and mid-brain noradrenaline levels reach their peak during the dark phase of the illumination cycle. These levels are significantly different from those found during the light phase of the illumination cycle. The rise in noradrenaline in the mid-brain of adrenalectomized rats, however, was not significant.5. Peak 5-hydroxytryptamine (5-HT) levels in the caudate nucleus of normal rats and the mid-brain of adrenalectomized rats were found to be 12 hr out of phase with peak values obtained for other parameters that were measured.6. The significance ofthese circadian rhythms in relation to states of sleep and wakefulness, general metabolism, and motor activity is discussed.

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“…The determination of amines was carried out according to Shore and Olin [10] with the modification of Mead and Finger [5], Three rat brains were used for each double-determination. 5HT was additionally determined according to Snyder et al [11].…”

Section: Methodsmentioning

confidence: 99%

Norepinephrine and 5-Hydroxytryptamine in the CNS of Rats under Continuous Illumination and Total Darkness

Rüther¹,

Halaris²,

Matussek³

1967

Pharmacology

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A single extraction method for the determination of both norepinephrine and serotonin in brain

Cited by 139 publications

References 0 publications

Rat brain amines, blood histamine and glucose levels in relationship to circadian changes in sleep induced by pentobarbitone sodium

Rat brain amines, blood histamine and glucose levels in relationship to circadian changes in sleep induced by pentobarbitone sodium

Circadian rhythms in rat mid‐brain and caudate nucleus biogenic amine levels

Norepinephrine and 5-Hydroxytryptamine in the CNS of Rats under Continuous Illumination and Total Darkness

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