Abstract:The performance characteristics of a newly developed liquid chromatography-mass spectrometry (LC-MS) method were validated and demonstrated to be fit for purpose in a pharmacokinetic and tissue depletion study of white-tailed deer and bison. Tulathromycin was extracted from bison and deer sera with acetonitrile or trifluoroacetic acid and K 2 HPO 4 (pH 6.8) buffer solution and cleaned up on a conditioned Bond-Elut cartridge. Tulathromycin, retained on the cartridge; it was eluted with methanol containing 2% fo… Show more
“…Its actions are related to direct inhibition of essential protein biosynthesis by selective binding to bacterial 50S ribosomal subunits, so it approved for use in the treatment and prevention of bovine respiratory disease and the treatment of swine respiratory disease [16]. Few LC/MS [17][18][19][20][21][22] and electrochemical method [23] were reported for the determination of TULA in in pharmaceutical formulations and or different biological tissues. Compared with the reported methods the proposed methods have some distinct advantages; the reported spectrophotometric methods [10][11][12][13] were less sensitive.…”
Section: Fig 1 Chemical Structure Of Ceftiofur and Tulathromycinmentioning
confidence: 99%
“…Compared with the reported methods the proposed methods have some distinct advantages; the reported spectrophotometric methods [10][11][12][13] were less sensitive. Although the reported HPLC and LC/MS methods [3][4][5][6][7][8][17][18][19][20][21][22] are sensitive enough, they are precluded due to higher cost and non-availability of such instruments in each laboratory in developing countries. No reported spectrophotometric methods were reported for determination of TULA in its dosage form.…”
Section: Fig 1 Chemical Structure Of Ceftiofur and Tulathromycinmentioning
Two simple, accurate and precise spectrophotometric methods were developed for the determination of two veterinary drugs, ceftiofur and tulathromycin in pure form as well as in pharmaceutical formulations. The first one (Method A) based on the reducing action of ceftiofur on Fe (ΙΙΙ) to Fe (ΙΙ) in its complex with 1, 10-phenanthroline (ferrin complex) to give the orange-red colored ferroin complex that exhibits an absorption maximum at 511 nm. Ceftiofur exhibited good linearity in the concentration range of 0.3-3.0 μg mL −1 .The second method (Method B) depended on formation of a binary complex between tulathromycin and eosin Y in in the presence of carboxy methylcellulose as surfactant. Under the optimum conditions, the binary complex showed absorption maxima at 556 nm. The method obeyed Beer's law over concentration range of 1.0-15.0 μg mL −1 . The proposed methods were used for determination of the studied drugs in pharmaceutical formulation; maxfur ® powder and draxxin ® injections with mean recoveries of 99.57and 99.71%, respectively. The validity of the methods was further proved by applying the standard addition technique. A proposal of the reactions pathways were described.
“…Its actions are related to direct inhibition of essential protein biosynthesis by selective binding to bacterial 50S ribosomal subunits, so it approved for use in the treatment and prevention of bovine respiratory disease and the treatment of swine respiratory disease [16]. Few LC/MS [17][18][19][20][21][22] and electrochemical method [23] were reported for the determination of TULA in in pharmaceutical formulations and or different biological tissues. Compared with the reported methods the proposed methods have some distinct advantages; the reported spectrophotometric methods [10][11][12][13] were less sensitive.…”
Section: Fig 1 Chemical Structure Of Ceftiofur and Tulathromycinmentioning
confidence: 99%
“…Compared with the reported methods the proposed methods have some distinct advantages; the reported spectrophotometric methods [10][11][12][13] were less sensitive. Although the reported HPLC and LC/MS methods [3][4][5][6][7][8][17][18][19][20][21][22] are sensitive enough, they are precluded due to higher cost and non-availability of such instruments in each laboratory in developing countries. No reported spectrophotometric methods were reported for determination of TULA in its dosage form.…”
Section: Fig 1 Chemical Structure Of Ceftiofur and Tulathromycinmentioning
Two simple, accurate and precise spectrophotometric methods were developed for the determination of two veterinary drugs, ceftiofur and tulathromycin in pure form as well as in pharmaceutical formulations. The first one (Method A) based on the reducing action of ceftiofur on Fe (ΙΙΙ) to Fe (ΙΙ) in its complex with 1, 10-phenanthroline (ferrin complex) to give the orange-red colored ferroin complex that exhibits an absorption maximum at 511 nm. Ceftiofur exhibited good linearity in the concentration range of 0.3-3.0 μg mL −1 .The second method (Method B) depended on formation of a binary complex between tulathromycin and eosin Y in in the presence of carboxy methylcellulose as surfactant. Under the optimum conditions, the binary complex showed absorption maxima at 556 nm. The method obeyed Beer's law over concentration range of 1.0-15.0 μg mL −1 . The proposed methods were used for determination of the studied drugs in pharmaceutical formulation; maxfur ® powder and draxxin ® injections with mean recoveries of 99.57and 99.71%, respectively. The validity of the methods was further proved by applying the standard addition technique. A proposal of the reactions pathways were described.
“…In the final manuscript Boison et al . describe a method that was validated and used for the depletion study of tulathromycin residues in bison and deer sera as well as selected tissues of white‐tailed deer.…”
“…Berendsen, Anton Kaufmann, John D.G. McEvoy, Leendert A. van Ginkel, Joe O. Boison, Els Daeseleire, Jin‐Wook Kwon, and Siska Croubels Funding statement: Travel by the Second Author to present this manuscript at SASKVAL III was funded by the OECD Co‐operative Research Programme: Biological Resource Management for Sustainable Agricultural Programmes.has been added to a paper by Joe O. Boison …”
mentioning
confidence: 99%
“…Berendsen, Anton Kaufmann, John D.G. McEvoy, Leendert A. van Ginkel, Joe O. Boison, Els Daeseleire, Jin‐Wook Kwon, and Siska Croubels …”
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