A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

Jacquot, Emmanuel; Tribodet, Michel; Croizat, Flora; Balme-Sinibaldi, Valérie; Kerlan, Camille

doi:10.1016/j.jviromet.2005.01.003

Cited by 31 publications

(9 citation statements)

References 44 publications

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“…From a molecular point of view, PVY-L26 appears as a variant within the PVY NTN strain. Some heterogeneity among PVY Z isolates occurs because PVY-L26 reacts with anti-PVY N antibodies (32; this study), consistent with previously determined PVY Z serological reactivity (R. Singh, personal communication), unlike the PVY Z isolate found in France, which was recognized by anti-PVY OC antibodies but not by anti-PVY N antibodies (40 (30,31,33,55) induced similar HRlike reaction in Maris Bard and mosaic in Desiree (C. Kerlan, unpublished). Other PVY Z isolates should now be characterized in the same manner in order to check whether L26 is unique or represents a larger PVY Z strain group; a recently reported isolate from Mexico, PVY-M3, may be a good second candidate for this characterization (54).…”

Section: Discussionsupporting

confidence: 88%

Identification of the Molecular Make-Up of the Potato virus Y Strain PVY^Z: Genetic Typing of PVY^Z-NTN

Kerlan¹,

Николаева²,

Hu³

et al. 2011

Phytopathology®

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Potato virus Y (PVY) strains were originally defined by interactions with different resistance genes in standard potato cultivars. Five distinct strain groups are defined that cause local or systemic hypersensitive responses (HRs) in genetic background with a corresponding N gene: PVY(O), PVY(N), PVY(C), PVY(Z), and PVY(E). The nucleotide sequences of multiple isolates of PVY(O) and PVY(N) differ from each other by ≈8% along their genomes. Additionally, complete genome sequences of multiple recombinant isolates are composed of segments of parental PVY(O) and PVY(N) sequences. Here, we report that recombinant isolate PVY-L26 induces an HR in potato 'Maris Bard' carrying the putative Nz gene, and is not recognized by two other resistance genes, Nc and Ny(tbr). These genetic responses in potato, combined with the inability of PVY-L26 to induce vein necrosis in tobacco, clearly define it as an isolate from the PVY(Z) strain group and provide the first information on genome structure and sequence of PVY(Z). The genome of PVY-L26 displays typical features of European NTN-type isolates with three recombinant junctions (PVY(EU-NTN)), and the PVY-L26 is named PVY(Z)-NTN. Three typical PVY(NTN) isolates and two PVY(N) isolates, all inducing vein necrosis in tobacco, were compared with PVY-L26. One PVY(NTN) isolate elicited HR reactions in Maris Bard, similar to PVY-L26, while two induced a severe systemic HR-like reaction quite different from the quasi-symptomless reaction induced by two PVY(N) isolates. 'Yukon Gold' potato from North America produced HR against several PVY(NTN) isolates, including PVY-L26, but only late and limited systemic necrosis against one PVY(N) isolate. Consequently, according to symptoms in potato indicators, both PVY(Z) and PVY(NTN) isolates appeared biologically very close and clearly distinct from PVY(O) and PVY(N) strain groups.

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Section: Discussionsupporting

confidence: 88%

Identification of the Molecular Make-Up of the Potato virus Y Strain PVY^Z: Genetic Typing of PVY^Z-NTN

Kerlan¹,

Николаева²,

Hu³

et al. 2011

Phytopathology®

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“…Specific detection of recombinant PVY NTN isolates was conducted with three primer pairs targeting the three recombination sites within HC-Pro/P3 region (primer pair 1227/46 Y N F/ 2547/68 Y O R), CI/NIa region (primer pair 5606/27 Y°F/ 6343/67 Y N R) and CP/3′NTR region (primer pair 9132/51 Y N F/ 9481/00 Y O R; Glais et al 2001; (Singh and Singh 1996), PVY N B203 (Glais et al 1998), PVY N Wi-P , PVY NTN NZ (Schubert et al 2007). B203 is an isolate of the PVY N group (pathotype and serotype N; Jacquot et al 2005) that does not elicit tuber necrosis in both natural and greenhouse conditions (Glais and Kerlan, unpublished). PVY NTN NZ is an isolate collected in New Zealand in 1999 from tubers cv.…”

Section: Methodsmentioning

confidence: 99%

Incidence and Characterization of Potato virus Y in Seed Potatoes in Tunisia

et al. 2010

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Surveys during five consecutive years in four main seed potatogrowing areas of Tunisia revealed large differences in Potato virus Y (PVY) incidence. Infection rates at harvest ranged from up to 19% in Cap Bon and in Jendouba, to 31% in Kairouan and 48% in Manouba. However, infection rates were very low across the country in 2003 (1-5%), except in Kairouan, and rather low in 2004 (4-10%). Secondary infections in plants growing from imported seed potatoes were assessed to be 1-6% depending on region and year. Serological analysis of a first set of 90 samples collected in 2004-2005 revealed dominance of the PVY N group (90% of the total number of PVY positives). A second set of 44 isolates from samples collected in 2006 was further analyzed with a combination of serotyping, indexing on tobacco, and RT-PCR tests targeting four genomic regions (5′Ntr/P1, HcPro/P3, CI/NIa, CP). Thirty-five of these 44 isolates were typical PVY NTN isolates, with three recombination junctions in HcPro/P3, CI/NIa, and CP regions, respectively, whereas no recombination junction was identified in the genome of five isolates belonging to the PVY N group. One additional recombinant PVY NTN isolate was recovered from a mixed infection with a PVY O isolate. Only three PVY O isolates were recovered from the samples analyzed.

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“…Further study on PVY/potato interactions should now be carried out to identify the plant product(s) interacting with the E419 of the HC-Pro protein. -Sinibaldi et al (2006), Glais and Jacquot (2015), Jacquot et al (2005), Kerlan et al (1999) and Rolland et al (2008).…”

Section: Discussionmentioning

confidence: 99%

The amino acid 419 in HC-Pro is involved in the ability of PVY isolate N605 to induce necrotic symptoms on potato tubers

et al. 2015

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The ability to induce the potato tuber necrosis ringspot disease (PTNRD) is a property shared by PVY isolates belonging to different groups (e.g. PVY(N) and PVY(O)) and variants (e.g. PVY(NTN) and PVY(N)-W). The identification of viral molecular determinant(s) involved in the expression of PTNRD symptoms is essential for (i) an easier detection of tuber necrosis isolates and (ii) an improvement of our knowledge on the epidemiology of this potato disease. A reverse genetic approach associated with a biological typing of a collection of PVY chimeras and mutants indicated that residue E419 of the HC-Pro protein is linked to the ability of PVY to induce tuber necrosis on four PTNRD-susceptible potato cultivars. Indeed, the substitution of the N-type glutamic acid (E) in O-type aspartic acid (D) at position 419 in the HC-Pro cistron prevents the expression of tuber necrosis on infected tubers without reducing the virulence of the corresponding E/D419 mutant. This result opens opportunities for the future studies on potato/PVY interactions.

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A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

Cited by 31 publications

References 44 publications

Identification of the Molecular Make-Up of the Potato virus Y Strain PVY^Z: Genetic Typing of PVY^Z-NTN

Identification of the Molecular Make-Up of the Potato virus Y Strain PVY^Z: Genetic Typing of PVY^Z-NTN

Incidence and Characterization of Potato virus Y in Seed Potatoes in Tunisia

The amino acid 419 in HC-Pro is involved in the ability of PVY isolate N605 to induce necrotic symptoms on potato tubers

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A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

Cited by 31 publications

References 44 publications

Identification of the Molecular Make-Up of the Potato virus Y Strain PVYZ: Genetic Typing of PVYZ-NTN

Identification of the Molecular Make-Up of the Potato virus Y Strain PVYZ: Genetic Typing of PVYZ-NTN

Incidence and Characterization of Potato virus Y in Seed Potatoes in Tunisia

The amino acid 419 in HC-Pro is involved in the ability of PVY isolate N605 to induce necrotic symptoms on potato tubers

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Identification of the Molecular Make-Up of the Potato virus Y Strain PVY^Z: Genetic Typing of PVY^Z-NTN

Identification of the Molecular Make-Up of the Potato virus Y Strain PVY^Z: Genetic Typing of PVY^Z-NTN