A Single Nucleotide Polymorphism in 3′-Untranslated Region Contributes to the Regulation of Toll-like Receptor 4 Translation

Sato, Kasumi; Yoshimura, Atsutoshi; Kaneko, Takashi; Ukai, Takashi; Ozaki, Yukio; Nakamura, H.; Li, Xinyue; Matsumura, Hideki; Hara, Yoshitaka; Ogata, Yorimasa

doi:10.1074/jbc.m111.338426

Cited by 68 publications

(62 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SNP rs11536889 is located in the 3'-untranslated region of TLR4, where a genetic change can influence the translation of TLR4 mRNA. Sato et al (2012) showed that rs11536889 can contribute to the regulation of TLR4 translation. They found that peripheral blood mononuclear cells from subjects carrying the C allele secreted higher levels of IL-8 than the cells from subjects with the wild-type genotype.…”

Section: Discussionmentioning

confidence: 99%

Pooled analysis of association between a genetic variant in the 3'-untranslated region of Toll-like receptor 4 and cancer risk

Wang

Miao

2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Many epidemiological studies have shown the association between certain genetic variations in the Toll-like receptor 4 (TLR4) gene (for example, rs4986790 and rs4986791) and cancer risk. However, the results from investigations into the association between rs11536889, a genetic variant in the 3'-untranslated region of TLR4, and cancer risk lack consensus. We performed a meta-analysis to investigate the effect of rs11536889 on cancer risk. A total of 12 relevant case-control studies were included in this analysis (6222 cases and 7948 controls). The pooled ORs with their corresponding 95%CIs were estimated. We did not detect any association between rs11536889 and overall cancer risk (P = 0.13). However, stratification analysis by cancer type revealed a borderline statistically significant increased risk in both genotype comparison (OR for the variant genotype in the dominant model = 1.17; 95%CI = 0.98-1.40; P = 0.08) and allele comparison (OR for variant allele = 1.14; 95%CI = 0.99-1.32; P = 0.07) for prostate cancer. In contrast, no statistically significant or borderline result was found for gastric cancer. These findings indicate that rs11536889 in TLR4 might be specifically associated with prostate 17848 X. Wang et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17847-17855 (2015) cancer. However, owing to the modest and underpowered results, and the limitations of the original studies included for analysis, further prospective studies with larger sample sizes are required to confirm our findings.

show abstract

Section: Discussionmentioning

confidence: 99%

Pooled analysis of association between a genetic variant in the 3'-untranslated region of Toll-like receptor 4 and cancer risk

Wang

Miao

2015

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…HGF were grown to confluence in 100-mm tissue culture dishes. The media were then changed to DMEM containing 1% FCS for 12 h, and HGF were incubated in this media with or without IL-1β (1 ng/mL) and TNF-α (10 ng/mL) for 24 h, as previously described (16)(17)(18). Total RNA was isolated from triplicate cultures and analyzed for expressions of miRNAs and U6 snRNA by real-time PCR, as described below.…”

Section: Cell Culturementioning

confidence: 99%

MicroRNA expression in inflamed and noninflamed gingival tissues from Japanese patients

et al. 2014

Self Cite

View full text Add to dashboard Cite

Periodontitis is a chronic inflammatory disease caused by specific bacteria and viruses. Local, systemic, and environmental factors affect the rate of disease progression. Immune responses to bacterial products, and the subsequent production of inflammatory cytokines, are crucial in the destruction of periodontal tissue. MicroRNAs (miRNAs) are a class of small RNAs that control various cell processes by negatively regulating protein-coding genes. In this study, we compared miRNA expression in inflamed and noninflamed gingival tissues from Japanese dental patients. Total RNAs were isolated from inflamed and noninflamed gingival tissues. miRNA expression profiles were examined by an miRNA microarray, and the data were analyzed by GeneSpring GX, Ingenuity Pathways Analysis, and the TargetScan databases. Observed miRNA expression levels in inflamed gingiva were confirmed by real-time PCR. The three most overexpressed (by >2.72-fold) miRNAs were hsa-miR-150, hsa-miR-223, and hsa-miR-200b, and the three most underexpressed (by <0.39-fold) miRNAs were hsa-miR-379, hsa-miR-199a-5p, and hsa-miR-214. In IPA analysis, hsa-miR-150, hsa-miR-223, and hsa-miR-200b were associated with inflammatory disease, organismal injury, abnormalities, urological disease, and cancer.

show abstract

“…The cells were cultured at 37°C in 5% CO 2 / 95% air in DMEM containing 10% FCS. They were grown to confluence in 100-mm tissue culture dishes, and the medium was then changed to DMEM containing 1% FCS for 12 h, followed by incubation in this medium with or without IL-1β (1 ng/mL), IL-6 (1 ng/mL), and TNF-α (10 ng/mL) for 24 h as described previously (13)(14)(15)(16)(17)(18)(19). Total RNA was isolated from quadruplicate cultures and analyzed for the expression of AMTN and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA by real-time PCR as described below.…”

Section: Cell Culturementioning

confidence: 99%

Proinflammatory cytokines induce amelotin transcription in human gingival fibroblasts

et al. 2014

Self Cite

View full text Add to dashboard Cite

A Single Nucleotide Polymorphism in 3′-Untranslated Region Contributes to the Regulation of Toll-like Receptor 4 Translation

Cited by 68 publications

References 36 publications

Pooled analysis of association between a genetic variant in the 3'-untranslated region of Toll-like receptor 4 and cancer risk

Pooled analysis of association between a genetic variant in the 3'-untranslated region of Toll-like receptor 4 and cancer risk

MicroRNA expression in inflamed and noninflamed gingival tissues from Japanese patients

Proinflammatory cytokines induce amelotin transcription in human gingival fibroblasts

Contact Info

Product

Resources

About