A single-nucleotide polymorphism in the proximal promoter region of the apolipoprotein M gene is associated with dyslipidaemia but not increased coronary artery diseases in Chinese populations

Abstract

BackgroundIt has been reported that rs940494 and rs805296 SNPs of apolipoprotein M (apoM) gene may confer the risk in the development of type 2 diabetes (T2D) and coronary artery disease (CAD) in the Han Chinese. However, a recent study demonstrated that rs805297 polymorphism is significantly associated with reduced total high density lipoprotein (HDL) levels in rheumatoid arthritis patients. But the relationship between rs805297 SNP and CAD has not been explored. The aim of the present study was to elucidate whether the rs805297 mutant allele is implicated in CAD and links to changes in blood lipid levels in these patients.MethodsThree hundred CAD patients and three hundred and twelve non-CAD patients were subjected in the present study. All subjects were confirmed by the angiography. Plasma concentrations of apoM were semi-quantitatively determined by dot-blotting analysis, and total serum lipid levels were quantified using an automated RA-1000 (Technician, USA). The genotyping of rs805297 of apoM was analyzed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP).ResultsGenotype and allele frequencies were not significant (P = 0.5798 and 0.3834, respectively) between cases and controls. Compared with the wild-type C/C genotype, carriers of the C/A and A/A genotypes did not have an increased risk of CAD, as determined by multiple logistic regression analysis, after adjustment for age, sex, BMI, history of smoking, hypertension and hypercholesterolemia. (CA, odds ratio = 0.49, 95% confidence interval 0.15–1.87, P = 0.462; AA, odds ratio = 0.51, 95% confidence interval 0.13–1.68, P = 0.534). The plasma concentration levels of apoM did not differ significantly among carriers of the three genotypes between two groups. Lastly, control subjects with A/A genotypes had lower total levels of HDL cholesterol than did those with C/C genotypes.ConclusionsThe results presented here suggest that the rs805297 SNP is not associated with an increased risk of developing CAD, although it does independently correlate with dyslipidaemia in Han Chinese individuals.

Beta2-Adrenergic Receptor Gene Polymorphisms in Egyptian Patients with Acute Myocardial Infarction

“…It is caused by myocardial cell death due to prolonged ischemia [3]. MI is a multifactorial, polygenic disorder driven by interactions of an individual's genetic background and several environmental factors [4][5][6][7]. Greater understanding of MI etiology is mandatory to identify individuals at high risk and to improve prevention and therapy of this common and important condition.…”

Identification of polymorphisms in Apolipoprotein M gene and their relationship with risk of recurrent venous thromboembolism

“…Similarly in ischaemic stroke, this polymorphism is reported to be associated with higher risk of disease development (39). Others, however, found no role of ApoM rs805297 polymorphism in development of coronary artery disease (40,41).…”

“…However, this hypothesis needs to be further investigated. Similarly, studies on CAD also showed that the ApoM rs805297 polymorphism was not associated with ApoM plasma levels (40,41). As the control of gene expression is a very complex phenomenon that is regulated at many levels, this inconsistency may arises from the fact that in addition to this polymorphism, there are many other factors that are involved in the expression of ApoM gene, i. e. transcription factors, microRNAs (miRNAs) and small interfering RNAs (siRNAs) (44,45).…”

The Effect of apoM Polymorphism Associated with HDL Metabolism on Obese Korean Adults

“…However, there are some controversies on the effects of those SNPs, with reports that support the different frequency distributions of SNPs among diverse races and nationalities [12,16,17]. More data on the effects of the apoM polymorphism with obesity and obesity-related diseases through future studies should be accumulated based on race in order to identify its causality.…”

“…Another study reported that the apoM T-778C SNP altered promoter activity and conferred susceptibility to type 1 diabetes in both Han Chinese and Swedish populations [15]. Although some studies reported that those SNPs did not significantly affect the development of those diseases [16,17], it is still valuable to investigate the relationship of apoM gene SNP with CAD and diabetes. ApoM T-855C and T-778C SNPs are found to be related with CAD and diabetes; however, the association of apoM polymorphism with obesity has not been reported.…”