A single nucleotide polymorphism in the coding region of bovine transferrin is associated with milk fat yield

Sanz, A.; Ordovás, Laura; Serrano, Claudia; Zaragoza, Pilar; Altarriba, J.; Rodellar, Clementina

doi:10.4238/vol9-2gmr784

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…Suggestive quantitative trait loci have been detected for fat yield at 50 cM (Casas et al, 2004). Sanz et al (2010) reported that SNP c.1455A>G in exon 12 of Tf had a significant association (P < 0.0006) with high fat production in Holstein-Friesian animals. However, in the present study, no significant associations of these SNPs with fat content were detected at the three loci in the populations analyzed (P > 0.05).…”

Section: Association Of Tf Gene Polymorphism With Milk and Health Tramentioning

confidence: 99%

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Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits

Zh¹,

Ql²,

Jm³

et al. 2011

Genet. Mol. Res.

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Section: Association Of Tf Gene Polymorphism With Milk and Health Tramentioning

confidence: 99%

“…Many polymorphisms have been found in the bovine Tf gene (Ashton et al, 1964;Zhang et al, 2008;Sanz et al, 2010). Twelve co-dominant alleles of Tf have been identified from a single locus using starch gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits

Zh¹,

Ql²,

Jm³

et al. 2011

Genet. Mol. Res.

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“…Single-nucleotide polymorphisms have been found in the Tf gene; however, due to limited availability of data on SNPs and their association with infectious diseases, particularly mastitis, they need to be examined further [16,17]. SNPs also need proper validation regarding whether they have any association with bovine mastitis resistance/tolerance.…”

Section: Introductionmentioning

confidence: 99%

In Silico Tools for Analysis of Single-Nucleotide Polymorphisms in the Bovine Transferrin Gene

Ali

Rehman

Ahmad

et al. 2022

Animals

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Dairy cattle with a high milk yield are susceptible to many infectious diseases, such as mastitis. Subclinical mastitis (SCM) is the most prevalent form of mastitis that predominantly affects animal health, and causes adverse effects on the quality and quantity of milk. In dairy animals, subclinical mastitis often remains undetected, as no gross changes in udder characteristics are visible. In the present study, 135 Holstein Friesian dairy animals were selected and screened as healthy (n = 25) and mastitic (n = 110) based on diagnostic tests such as the California mastitis test, pH, electrical conductivity, and somatic cell count. In this study, the somatic cell count was used as a gold-standard test in differentiating subclinical mastitis animals from healthy ones. The present study was carried out to study polymorphisms in the bovine transferrin gene in cows (with subclinical mastitis and healthy). For the early detection of resistant/or susceptible animals, a useful marker could be provided by the detection of single-nucleotide polymorphisms (SNPs) in the transferrin gene, which are often associated with mammary innate immune response. The sequencing results revealed three nucleotide substitutions: two transversions (230 A > C, 231 C > A) and one transition (294 A > G) in susceptible cows as compared to disease-free subjects. The nucleotide variations at position 230 (GAC > GCA) and 231 (GAC > GCA) were nonsynonymous, and corresponded to an amino acid change from aspartic acid to alanine; whereas at position 294 (GAA > GAG), the mutation was synonymous. In the present study, many in silico tools were taken into consideration to determine the effect of SNPs on protein structure and function. The PROVEAN tool found the amino acid substitution to be neutral and deleterious. PolyPhen-2 revealed the amino acid variations at positions 320 and 321 to most likely be damaging; and at the 341 position, the variations were benign. The I-Mutant and MUpro tools found that the protein stability decreased for nonsynonymous variations. The SIFT tool revealed the protein function was likely to be affected in nonsynonymous variations, with no change in the case of synonymous ones. Phylogenetic analysis of the bovine transferrin gene revealed a close relation of the CA allele with the Bos taurus transferrin, while the G allele was closely related to a cross of Bos indicus × Bos taurus serotransferrins, followed by the Bison bison transferrin. The least relation was shown by both alleles to Capra hircus, Ovis aries, and Bubalus bubalis.

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“…It has been shown that genes, connected originally to health and development traits, like IL8, IL8R, CCL2, CCR2, can be associated with dairy performance. Polymorphisms found in the above-mentioned genes are connected to at least one of the five main performance traits: milk, protein and fat yield, protein and fat percentage (LeyvaBaca et al 2007, Chen et al 2010, Sanz et al 2010. Lactoferrin (LF), an iron-chelating protein, is present in many mammalian biological fluids, including milk.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it is of great importance to discover associations between genes related with animal health and performance simultaneously. Other genes, like IGF1 and LF »ancestor gene« -TF, were proved to influence milk performance, despite their primary role in health, development or reproduction (Sanz et al 2010, Szewczuk et al 2012). This suggests the pleiotropic effect, or the existence of a linkage between genes coding for the non-productive traits and dairy performance traits.…”

Section: Introductionmentioning

confidence: 99%

Association between lactoferrin single nucleotide polymorphisms and milk production traits in Polish Holstein cattle

et al. 2014

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Bovine lactoferrin exhibits strong potential for further applications as a mastitis resistance marker. Since selection for mastitis resistance should not interfere with dairy performance, we investigated the association between bovine lactoferrin gene polymorphism and production traits in Polish Holsteins. The associations between four SNPs, localized in the 5'-flanking region and in exons 4 and 9 of the lactoferrin gene, and dairy performance were examined. SNPs were associated with almost all test-day milk performance traits. Significant associations were found between lactoferrin genotypes and the estimated breeding values for those traits. To find out whether the discrepancies between the lactoferrin gene SNP's influence on phenotype (test-day milk performance) and on estimated breeding values originate from the impact of other factors, we explored the genotype by environment interaction. Substantial impacts of SCC, lactation stage and parity were found. This paper suggests that the genotype by environment interaction may significantly change associations between genes and traits. It is important to include similar analyses to the studies on disease markers before using them in the selection.

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A single nucleotide polymorphism in the coding region of bovine transferrin is associated with milk fat yield

Cited by 9 publications

References 14 publications

Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits

Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits

In Silico Tools for Analysis of Single-Nucleotide Polymorphisms in the Bovine Transferrin Gene

Association between lactoferrin single nucleotide polymorphisms and milk production traits in Polish Holstein cattle

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