A Single-Sample Method for Determination of Carbohydrate and Protein Contents Glycoprotein Bands Separated by Sodium Dodecyl Sulfate– Polyacrylamide Gel Electrophoresis

Analyzing Sialic Acids Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

“…Figure 1B shows a sialic acid analysis of human serum transferrin in which HCl was the hydrolysis acid. Dilute trifluoroacetic acid has also been used (13,27,30,32,38,42). Acetic acid hydrolysis (e.g., 2 M at 80°C for 3 h) improves the recovery of O-substitutions found on some sialic acids (49) and has been used to release sialic acids prior to HPAE-PAD analysis (30,38,41,42,50).…”

“…Sialic acids were determined from 8 g of recombinant erythropoietin that was first separated by gel electrophoresis and then electroblotted to a polyvinylidene fluoride membrane (21). The sialic acid contents of other glycoproteins have been determined in the same manner (22,32).…”

A Comparison of Fluorescamine and Naphthalene-2,3-dicarboxaldehyde Fluorogenic Reagents for Microplate-Based Detection of Amino Acids

“…Although these assays are extremely rapid and convenient, O'Reilly and Lanza (9) have demonstrated that it is possible for fluorescamine to introduce significant error due to differential fluorescence intensities of derivatized amino acids. In the fluorometric determination of total proteins, such as melittin (13), oxidized insulin ␤-chain (14), band 3 glycoprotein, albumin, and transferrin (15), amino acids released upon protein hydrolysis also need to be accurately quantified.…”

Mannose 6-Phosphate Quantitation in Glycoproteins Using High-pH Anion-Exchange Chromatography with Pulsed Amperometric Detection

“…Previous reports have demonstrated sensitive assays for sialic acid and monosaccharides from glycoprotein electroblots using HPAEC-PAD (22,24,25). The current study explored this potential use for the mannose 6-phosphate assay.…”