A Single-Tube RT-PCR Amplification for Detection of Rift Valley Fever Virus

Salim, Reham W.; Khairalla, Khairalla M S; Eljamal, Awadalkareem A.; Karrar, A. E.; Aradaib, Imadeldin E.

doi:10.3923/rjmsci.2010.146.151

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Step RT-PCR for detection of RVFV (QIAGen, California, USA): A single tube RT-PCR amplification was carried out using One Step Access RT-PCR system (Table 3) according to Salim et al (2010).…”

Section: Onementioning

confidence: 99%

“…The primers specific for amplification of RVFV M segment were designed based on multiple sequences alignment of 20 published conserved sequences of the genes using Bio Eidit software (Carlsbad, CA, USA). The RT-PCR would produce a 342 bp PCR product, Primers included bases of 2163-2182of the positive sense strand of the M RNA gene 5-CTGTCTGGCACAGCATTGAT-3 in RV1, and the complementary strand included 2485-2504 bases 5-CACATTGAAACACCCACACC-3 in RV2 according to Salim et al (2010). The detection percentages of RVFV IgG antibodies among sheep<1year; 1-3years; 3-5years old and >5years old were 14.2; 23.3; 25 and 28.5%, respectively.…”

Section: Primers Selectionmentioning

confidence: 99%

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Some Associated Risk Factors With the Occurrence of Rift Valley Fever in Animals and Man in Certain Localities of Nile Delta, Egypt

2014

Assiut Veterinary Medical Journal

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Rift Valley Fever (RVF) is acute viral mosquito-borne disease of Ruminants and man that can cause epizootics and associated human epidemics. This study was carried out for detection of RVFV IgG in animals and man and to investigate the occurrence of RVFV in mosquitoes. A total of 460 animal blood samples were collected from vaccinated and non vaccinated sheep (172), goats (48), cattle (164) and buffaloes (76) from different farms, flocks and abattoirs in Menoufia, Behera and Kafr El Sheikh Governorates. All samples were examined by indirect Enzyme Linked Immunosorbant Assay, the overall detection percentages of RVFV IgG antibodies were 14.9% in non-vaccinated animals and 19.2% in vaccinated animals, respectively and it was found that Kafr El sheikh had the higher percentage than the other two Governorates (21.4 %). Also sheep and goat represented the most principle host to RVF by a parentages of 21.4% and 18.7 % respectively, especially on the age group > 3-5 years by (17.9%). Moreover, female animals had the higher percentage (17.7%) than males (8.6%). Furthermore this study proved that RVF play an important role in abortions especially in sheep and goats. In human, a total of 369 human blood samples collected from fever hospitals and clinical laboratories in the three Governorates. It was found that males contacted to animals such as sheepherders, farmers and butchers showed higher percentage (12.9%) than females, especially in the age group 30-45 years by a percentage of 16.6%. This results potentiate the role of animals in transmission of disease to man. Concerning mosquitoes, a total of 872 mosquitoes were collected by using CDC light trap and identified into 32 mosquito pool according to genus Culex, Aedes and Anopheles, culex constituted the higher percentages of 71.8%. All mosquito samples did not give any band at the expected size 342 bp by RT-PCR. This negative result did not mean that the virus completely absent as might be present in a low level in the study area or might be present in other Governorates in Egypt.

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Section: Onementioning

confidence: 99%

Section: Primers Selectionmentioning

confidence: 99%

Some Associated Risk Factors With the Occurrence of Rift Valley Fever in Animals and Man in Certain Localities of Nile Delta, Egypt

2014

Assiut Veterinary Medical Journal

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Molecular detection of Rift Valley fever virus in serum samples from selected areas of Tanzania

Chengula

Kasanga

Mdegela

et al. 2014

Trop Anim Health Prod

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Rift Valley fever (RVF) is an acute mosquito-borne viral zoonotic disease affecting domestic animals and humans caused by the Rift Valley fever virus (RVFV). The virus belongs to the genus Phlebovirus of the family Bunyaviridae. The main aim of this study was to detect the presence of antibodies to RVFV as well as the virus in the serum samples that were collected from livestock during the 2006/2007 RVF outbreaks in different locations in Tanzania. Analysis of selected samples was done using a RVF-specific inhibition enzyme-linked immunosorbent assay (I-ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Genomic viral RNA was extracted directly from serum samples using a QIAamp Viral RNA Mini Kit (QIAGEN), and a one-step RT-PCR protocol was used to amplify the S segment of RVFV. Positive results were obtained in 39.5% (n = 200) samples using the RVF I-ELISA, and 17.6% (n = 108) of samples were positive by RT-PCR. I-ELISA detected 41 (38.7%), 32 (39.0%), and 6 (50.0%) positive results in cattle, goats, and sheep sera, respectively, whereas the RT-PCR detected 11 (0.2%), 7 (0.2%), and 1 (0.1%) positive results in cattle, goats, and sheep sera, respectively. These findings have demonstrated the presence of RVFV in Tanzania during the 2006/2007 RVF outbreaks. To our knowledge, this is the first report to detect RVFV in serum samples from domestic animals in Tanzania using PCR technique. Therefore, a detailed molecular study to characterize the virus from different geographical locations in order to establish the profile of strains circulating in the country and develop more effective and efficient control strategies should be done.

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Rift Valley Fever Virus Large (L) RNA Segment as a Target Gene for Direct Detection of the Virus in Acute Phase Sera Using RT-PCR

Elata¹,

Aradaib²

2011

International J. of Tropical Medicine

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A Single-Tube RT-PCR Amplification for Detection of Rift Valley Fever Virus

Cited by 3 publications

References 19 publications

Some Associated Risk Factors With the Occurrence of Rift Valley Fever in Animals and Man in Certain Localities of Nile Delta, Egypt

Some Associated Risk Factors With the Occurrence of Rift Valley Fever in Animals and Man in Certain Localities of Nile Delta, Egypt

Molecular detection of Rift Valley fever virus in serum samples from selected areas of Tanzania

Rift Valley Fever Virus Large (L) RNA Segment as a Target Gene for Direct Detection of the Virus in Acute Phase Sera Using RT-PCR

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