A SIRT3/AMPK/autophagy network orchestrates the protective effects of trans-resveratrol in stressed peritoneal macrophages and RAW 264.7 macrophages

Duan, Wen-Jun; Li, Yi-Fang; Liu, Fanglan; Deng, Jie; Wu, Yanping; Yuan, Wei-Lin; Tsoi, Bun; Chen, Junli; Wang, Qi; Cai, Shaohui; Kojima, Hiroshi; He, Rong‐Rong

doi:10.1016/j.freeradbiomed.2016.03.022

Cited by 76 publications

(64 citation statements)

References 56 publications

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“…CXCL15 attracts neutrophils into the inflamed sites, favouring pro‐inflammatory M1 macrophage polarization . To investigate whether these mediators are modulated by Res, we measured IL‐1beta and CXCL5 expression in the lung tissues by ELISA (Figure A‐B) and qRT‐PCR analysis (Figure C).…”

Section: Resultsmentioning

confidence: 99%

“…It is documented that SIRT1 is critically involved in variable cell biological function . Loss of SIRT1 in SIRT1 knock‐out mice delayed locomotor recovery, in association with more expression of pro‐inflammatory cytokines and population of M1 type macrophages in a mouse model with spinal cord injury .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, we observed lower expression of IL-6 and CXCL15 ( Figure 7E), but higher expression of SIRT1 and arginase-1 ( Figure 7C It is documented that SIRT1 is critically involved in variable cell biological function. [22][23][24] Loss of SIRT1 in SIRT1 knock-out mice delayed locomotor recovery, in association with more expression of pro-inflammatory cytokines and population of M1 type macrophages in a mouse model with spinal cord injury. 25 Consistent with the previous report, 22 we observed that Res up-regulated the expression of SIRT1, whereas LPS moderately reduced the expression of SIRT1 in BMDMs.…”

Section: Res Suppressed M1 Cell-biased Polarization Of Bmdms From Wmentioning

confidence: 99%

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Resveratrol decreases CD45⁺CD206⁻ subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Chen

et al. 2019

J Cellular Molecular Medi

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Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) are life‐threatening condition in critically ill patients. Resveratrol (Res), a natural polyphenol, has therapeutic effect in animal model with ALI; however, whether Res attenuates ALI through modulation of macrophage phenotypes in the animal model remains unknown. We in this study treated LPS‐induced murine ALI with 30 mg/kg Res and observed significantly reduced severity of ALI in the Res‐treated mice 48 hours after Res treatment. Neutrophil infiltrates were significantly reduced, accompanied with lower infiltration of CD45+Siglec F− phenotype macrophages, but higher population of CD45+Siglec F+ and CD45+CD206+ alternatively activated macrophages (M2 cells) in the Res‐treated mice with ALI. In addition, the expression of IL‐1beta and CXCL15 cytokines was suppressed in the treated mice. However, Res treatment in mice with myeloid cell‐restricted SOCS3 deficiency did not significantly attenuate ALI severity and failed to increase population of both CD45+Siglec F+ and CD45+CD206+ M2 subtype macrophages in the murine ALI. Further studies in wild‐type macrophages revealed that Res treatment effectively reduced the expression of IL‐6 and CXCL15, and increased the expression of arginase‐1, SIRT1 and SOCS3. However, macrophages’ lack of SOCS3 expression were resistant to the Res‐induced suppression of IL‐6 and CXCL15 in vitro. Thus, we conclude that Res suppressed CD45+Siglec F− and CD45+CD206− M1 subtype macrophages through SOCS3 signalling in the LPS‐induced murine ALI.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Res Suppressed M1 Cell-biased Polarization Of Bmdms From Wmentioning

confidence: 99%

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Resveratrol decreases CD45⁺CD206⁻ subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Chen

et al. 2019

J Cellular Molecular Medi

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“…In endothelial cells, EGCG reduced palmitate-induced lipid droplet storage through inducing autophagy via a Ca2+/CaMKKβ/AMPK-mediated mechanism [38]. And resveratrol promoted autophagy via upregulating SIRT3 expression and AMPK phosphorylation in macrophages [42]. Therefore, AMPK may play a role in the autophagy-inducing properties of GTPs in renal protection, which is similar to that of DR.…”

Section: Discussionmentioning

confidence: 99%

Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux

Xie

Zhang

et al. 2017

Nutrients

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Epidemiological and experimental studies reveal that Western dietary patterns contribute to chronic kidney disease, whereas dietary restriction (DR) or dietary polyphenols such as green tea polyphenols (GTPs) can ameliorate the progression of kidney injury. This study aimed to investigate the renal protective effects of GTPs and explore the underlying mechanisms. Sixty Wistar rats were randomly divided into 6 groups: standard diet (STD), DR, high-fat diet (HFD), and three diets plus 200 mg/kg(bw)/day GTPs, respectively. After 18 weeks, HFD group exhibited renal injuries by increased serum cystatin C levels and urinary N-acetyl-β-d-glucosaminidase activity, which can be ameliorated by GTPs. Meanwhile, autophagy impairment as denoted by autophagy-lysosome related proteins, including LC3-II, Beclin-1, p62, cathepsin B, cathepsin D and LAMP-1, was observed in HFD group, whereas DR or GTPs promoted renal autophagy activities and GTPs ameliorated HFD-induced autophagy impairment. In vitro, autophagy flux suppression was detected in palmitic acid (PA)-treated human proximal tubular epithelial cells (HK-2), which was ameliorated by epigallocatechin-3-gallate (EGCG). Furthermore, GTPs (or EGCG) elevated phosphorylation of AMP-activated protein kinase in the kidneys of HFD-treated rats and in PA-treated HK-2 cells. These findings revealed that GTPs mimic the effects of DR to induce autophagy and exert a renal protective effect by alleviating HFD-induced autophagy suppression.

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“…AAPH is an azo compound and completely dissolved in alkyl, peroxyl and alkoxyl radicals at physiological temperature [11]. AAPH has been widely used to study the mechanism of oxidative stress [12,13]. In our previous experiments, the chick embryo on embryo development day (EDD) 9 was treated with a large dose of AAPH.…”

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confidence: 99%

Edaravone Protects against Vascular Oxidative Damage Induced by AAPH in Chick Embryo

Wan¹,

Luo²,

Jie³

et al. 2016

Int J Pharm Sci Dev Res

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Background: Edaravone (Eda) is a free-radical scavenger which is used in treating stroke, cerebral hemorrhage and some other diseases in clinic. However, it's antioxygenation during development is still unknown.Method: An oxidative damage model was established in chick embryo by using a generator of free radicals, 2,2'-azobis[2-methylpropionamidine] dihydrochloride (AAPH). The mortality rate and embryo's weight were measured to detect whether retarded growth happened. In order to observe the blood vessels, the yolk-sac blood vessels and CAM blood vessels density were observed. The malondialdehyde (MDA) content and superoxide dismutase (SOD) enzymatic activity were detected to evaluate the oxidative damage in the chick embryo.Result: In this model, embryo development and angiogenesis were heavily affected by AAPH. However, Eda alleviated the growth retardation and anti-angiogenesis induced by AAPH. After AAPH treatment, the MDA content increased and SOD enzymatic activity declined which was also mitigated by Eda. Conclusion:Overall, our research revealed that Eda mitigated embryonic anti-angiogenesis induced by oxidative damage after AAPH treatment, which may be helpful for future clinical studies.stress [7]. During the pregnancy period, pregnant women suffer from emotional fluctuation, environmental pollution, life pressure, improper diet and so on. Women are exposed to oxidative stress and the fetuses are easily affected.In this research, the chick embryo was chosen as the experimental animal, as chick embryo is suitable for evaluating angiogenesis. The chick embryo holds a wide range of advantages, with low cost and less ethical arguments [8]. It is a classical development model where the chick yolk sac and chorioallantoic membranes (CAMs) are extensively used in research related to angiogenesis. Besides, the growth cycle of chick embryo is short, where 21 days for chick embryo is similar to 10 months for human [9]. Moreover, it is a simple spinal biological, whose growth process can be easily observed [10].In order to mimic chronic oxidative stress exposed to human, 2,2'-azobis[2-methylpropionamidine] dihydrochloride (AAPH) was repeatedly treated in a small dose. AAPH is an azo compound and completely dissolved in alkyl, peroxyl and alkoxyl radicals at physiological temperature [11]. AAPH has been widely used to study the mechanism of oxidative stress [12,13]. In our previous experiments, the chick embryo on embryo development day (EDD) 9 was treated with a large dose of AAPH. After incubation for 24 hours, the chick embryo suffered from oxidative stress and the cardiovascular system was affected [7]. Nevertheless, whether chronic oxidative stress affects the development of embryos is still unclear. In our study, AAPH was injected to chick embryos in long-term and the development of chick embryo was observed. AbbreviationsEda: Edaravone; 3-methyl-1-phenyl-2-pyrazolin-5-one; AAPH: 2,2'-azobis [2-methylpropionamidine] IntroductionEdaravone (3-methyl-1-phenyl-2-pyrazolin-5-one, Eda), a free-radical sc...

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A SIRT3/AMPK/autophagy network orchestrates the protective effects of trans-resveratrol in stressed peritoneal macrophages and RAW 264.7 macrophages

Cited by 76 publications

References 56 publications

Resveratrol decreases CD45⁺CD206⁻ subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Resveratrol decreases CD45⁺CD206⁻ subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux

Edaravone Protects against Vascular Oxidative Damage Induced by AAPH in Chick Embryo

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A SIRT3/AMPK/autophagy network orchestrates the protective effects of trans-resveratrol in stressed peritoneal macrophages and RAW 264.7 macrophages

Cited by 76 publications

References 56 publications

Resveratrol decreases CD45+CD206− subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Resveratrol decreases CD45+CD206− subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux

Edaravone Protects against Vascular Oxidative Damage Induced by AAPH in Chick Embryo

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Resveratrol decreases CD45⁺CD206⁻ subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway

Resveratrol decreases CD45⁺CD206⁻ subtype macrophages in LPS‐induced murine acute lung injury by SOCS3 signalling pathway