A small-scale method for the preparation of plant N-linked glycans from soluble proteins for analysis by MALDI-TOF mass spectrometry

Karg, Saskia R.; Frey, Alexander D.; Ferrara, Claudia; Streich, Daniel K.; Umaña, Pablo; Kallio, Pauli T.

doi:10.1016/j.plaphy.2008.10.005

Cited by 17 publications

(5 citation statements)

References 38 publications

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“…Thus, it can be essential in some instances to characterize the sugar moieties of recombinantly expressed proteins in detail to assess product consistency as well as to understand the relationship between glycan structure and function. Several methods have been described for detailed characterization of the glycan profiles of proteins, ranging from HPLC over capillary electrophoresis to MS‐based methods [94].…”

Section: Quality‐control Criteria and Methodologies Appliedmentioning

confidence: 99%

Space–time fluid–structure interaction modeling of patient‐specific cerebral aneurysms

Tezduyar

Takizawa

Brummer

et al. 2011

Numer Methods Biomed Eng

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SUMMARYWe provide an extensive overview of the core and special techniques developed earlier by the Team for Advanced Flow Simulation and Modeling (T AFSM) for space-time fluid-structure interaction (FSI) modeling of patient-specific cerebral aneurysms. The core FSI techniques are the Deforming-SpatialDomain/Stabilized Space-Time (DSD/SST) formulation and the stabilized space-time FSI (SSTFSI) technique. The special techniques include techniques for calculating an estimated zero-pressure (EZP) arterial geometry, a special mapping technique for specifying the velocity profile at an inflow boundary with non-circular shape, techniques for using variable arterial wall thickness, mesh generation techniques for building layers of refined fluid mechanics mesh near the arterial walls, a recipe for pre-FSI computations that improve the convergence of the FSI computations, the Sequentially-Coupled Arterial FSI (SCAFSI) technique and its multiscale versions, techniques for the projection of fluid-structure interface stresses, calculation of the wall shear stress (WSS) and calculation of the oscillatory shear index (OSI) and arterial-surface extraction and boundary condition techniques. We show how these techniques work with results from earlier computations. We also describe the arterial FSI techniques developed and implemented recently by the T AFSM and present a sample from a wide set of patient-specific cerebral-aneurysm models we computed recently.

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Section: Quality‐control Criteria and Methodologies Appliedmentioning

confidence: 99%

Space–time fluid–structure interaction modeling of patient‐specific cerebral aneurysms

Tezduyar

Takizawa

Brummer

et al. 2011

Numer Methods Biomed Eng

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“…N -glycans were prepared and purified from the mutant and wild-type rice leaves according to Karg et al [ 55 ]. The N -glycan analysis was performed in positive-ion reflectron mode using a matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF MS) with an Autoflex system from Bruker Daltonics (Bruker, Billerica, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice

Sim

Kesawat

Kumar

et al. 2018

IJMS

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N-linked glycosylation is one of the key post-translational modifications. α1,3-Fucosyltransferase (OsFucT) is responsible for transferring α1,3-linked fucose residues to the glycoprotein N-glycan in plants. We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions. In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant. Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that α1,3-fucose was lacking in the N-glycan structure of the mutant. Mutant complementation revealed that the phenotype was caused by loss of Osfuct function. Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins. Moreover, the mutant exhibited sensitivity to an increased concentration of salt. This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.

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“…In general, PNGase A and F are available to release N-glycans. However, PNGase F cannot release N-glycans containing α1,3-linked Fuc from proteins (Karg et al, 2009), but PNGase A can cleave α 1,3-linked Fuc. Further treatment with PNGase A and F could enzymatically release the N-glycans of okra glycoprotein.…”

Section: Characterisation Of N-glycan Structure By Maldi-tof-msmentioning

confidence: 99%

Glycan characterisation and antioxidant activity of a novel N-linked glycoprotein from okra

Zhao,

Xu,

Chen

et al. 2021

IFRJ

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Glycoproteins are present in all living beings, and have many biological functions. The characterisation of glycan structures of plant glycoproteins has become increasingly important in biotechnology and agricultural applications. In the present work, the antioxidant activities of the okra glycoprotein were assessed. The glycan structures of the okra glycoprotein were analysed using lectin microarray combined with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The okra glycoprotein showed relatively strong 2,2-diphenyl-1-picrylhydrazyl-scavenging ability and reducing power. In addition, the glycan structures of the okra glycoprotein mainly contained N-acetylglucosamine, mannose, and galactose. Furthermore, complex-type N-glycans were the major type of glycan structures from the okra glycoprotein. Most of the complex N-glycans of the okra glycoprotein had terminal GalNAc and Gal N-glycan structures; the glycoprotein showed a high level of fucosylated complex-type glycans. Therefore, the okra glycoprotein is a promising antioxidant. Results of the present work might serve as a reference for a better understanding of the structural information and bioactivity of okra glycoprotein.

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A small-scale method for the preparation of plant N-linked glycans from soluble proteins for analysis by MALDI-TOF mass spectrometry

Cited by 17 publications

References 38 publications

Space–time fluid–structure interaction modeling of patient‐specific cerebral aneurysms

Space–time fluid–structure interaction modeling of patient‐specific cerebral aneurysms

Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice

Glycan characterisation and antioxidant activity of a novel N-linked glycoprotein from okra

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