A small-sized benzothiazole–indolium fluorescent probe: the study of interaction specificity targeting c-MYC promoter G-quadruplex structures and live cell imaging

Zheng, Bo; She, Meng Ting; Long, Wei; Xu, Yong; Zhang, Yi Han; Huang, Xuan He; Liu, Wenjie; Hou, Jin Qiang; Wong, Wing‐Leung; Lu, Yu

doi:10.1039/d0cc06525k

Cited by 23 publications

(22 citation statements)

References 23 publications

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“…Numerous research studies proved that the G-quadruplexes are abundant in the promoter regions of oncogenes like c-MYC. 25,26 The regulation process of early promoters of alphaherpes viruses that affects the human nervous system by G-motifs was clearly explained by Frasson et al 27 The conservation of G-quadruplexes and their evolution in mammals and non-mammal species were spelled out by using QGRS (Quadruplex forming G-rich sequences)-Conserve method. 28, 29 The presence of putative G4 and C-rich sequences in plants and mammals may help understand the inhibitory actions of binding proteins and ligands especially at telomeric regions, which facilitate finding novel therapeutic effects.…”

Section: L-112mentioning

confidence: 99%

Dissecting G-Quadruplexes and I-Motifs in Cis-Regulatory Regions of Mammals and Plants

Renuka

Yella

2022

Int J Life Sci Pharm Res

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The evolutionary constraints of a gene consist of cis-gene regulatory regions, such as promoters and enhancers, which contribute to the regulation of gene expression. Several genomic and computational studies stated the roles of G-quadruplex and i-motif structures in vital cellular processes like transcription, translation, gene regulation, etc. The formation of these non-B DNA structures is supported by the occurrence of unique repeated sequences. However, many studies lean toward understanding the role of the G-quadruplex, and only recent studies indicated i-motif significance. In this study, we attempted to dissect the enrichment of G-quadruplex and i-motifs in promoter regions of mammals and plants. To this end, we employed the genomic sequences encompassing -500 to +500 region relative to the gene start positions in mammals and plants retrieved from the UCSC browser and Plant Genome database (PlantGDB). We computed the putative G-quadruplexes and i-motifs with well-recognized regular expression sequence patterns. We observed that G-quadruplex motifs showed preponderance in mammals, algal species, namely, green algae, and Chlamydomonas when compared to plants. Contrastingly i-motifs are enriched in both monocot and dicot plants compared to G-quadruplex motifs. The comparative examinations in this study revamp our understanding of the two quadruplex structures and their emerging functional roles in complex eukaryotes.

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Section: L-112mentioning

confidence: 99%

Dissecting G-Quadruplexes and I-Motifs in Cis-Regulatory Regions of Mammals and Plants

Renuka

Yella

2022

Int J Life Sci Pharm Res

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“…Notably, the distinct abundance of G-prone sequences was detected in the promoters of oncogenes, suggesting the potential to use G-quadruplex regulation in cancer treatment [47,59]. Probably the best explored G-quadruplex in promoters is a sequence from the human c-Myc oncogene [60,61]. Regulatory sequences of this gene contain G-rich regions that can adopt a noncanonical DNA structure, as demonstrated both in vitro and in vivo, where its formation serves as an important regulator of c-Myc expression [35].…”

Section: Promoter Structure Motifsmentioning

confidence: 99%

Evolution of Diverse Strategies for Promoter Regulation

2021

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“…In addition, BZT-Indolium is a fluorescent and photostable probe that is highly specific for c-MYC G4. BZT-Indolium downregulates c-MYC transcription and can be used for in vitro staining and live cell imaging [50]. Recent strategies to identify new c-MYC G4 ligands include high-throughput screenings.…”

Section: Selective G4 Ligandsmentioning

confidence: 99%

Quadruplex Ligands in Cancer Therapy

Sánchez-Martín

Soriano

García‐Salcedo

2021

Cancers

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Nucleic acids can adopt alternative secondary conformations including four-stranded structures known as quadruplexes. To date, quadruplexes have been demonstrated to exist both in human chromatin DNA and RNA. In particular, quadruplexes are found in guanine-rich sequences constituting G-quadruplexes, and in cytosine-rich sequences forming i-Motifs as a counterpart. Quadruplexes are associated with key biological processes ranging from transcription and translation of several oncogenes and tumor suppressors to telomeres maintenance and genome instability. In this context, quadruplexes have prompted investigations on their possible role in cancer biology and the evaluation of small-molecule ligands as potential therapeutic agents. This review aims to provide an updated close-up view of the literature on quadruplex ligands in cancer therapy, by grouping together ligands for DNA and RNA G-quadruplexes and DNA i-Motifs.

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A small-sized benzothiazole–indolium fluorescent probe: the study of interaction specificity targeting c-MYC promoter G-quadruplex structures and live cell imaging

Abstract: A small-sized c-MYC promoter G-quadruplex selective fluorescent BZT-Indolium binding ligand was demonstrated for the first time as a highly target-specific and photostable probe for in vitro staining and live cell...

Cited by 23 publications

References 23 publications

Dissecting G-Quadruplexes and I-Motifs in Cis-Regulatory Regions of Mammals and Plants

Dissecting G-Quadruplexes and I-Motifs in Cis-Regulatory Regions of Mammals and Plants

Evolution of Diverse Strategies for Promoter Regulation

Quadruplex Ligands in Cancer Therapy

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