This paper presents a new framework for object-based classification of high-resolution hyperspectral data. This multi-step framework is based on multi-resolution segmentation (MRS) and Random Forest classifier (RFC) algorithms. The first step is to determine of weights of the input features while using the object-based approach with MRS to processing such images. Given the high number of input features, an automatic method is needed for estimation of this parameter. Moreover, we used the Variable Importance (VI), one of the outputs of the RFC, to determine the importance of each image band. Then, based on this parameter and other required parameters, the image is segmented into some homogenous regions. Finally, the RFC is carried out based on the characteristics of segments for converting them into meaningful objects. The proposed method, as well as, the conventional pixel-based RFC and Support Vector Machine (SVM) method was applied to three different hyperspectral data-sets with various spectral and spatial characteristics. These data were acquired by the HyMap, the Airborne Prism Experiment (APEX), and the Compact Airborne Spectrographic Imager (CASI) hyperspectral sensors. The experimental results show that the proposed method is more consistent for land cover mapping in various areas. The overall classification accuracy (OA), obtained by the proposed method was 95.48, 86.57, and 84.29% for the HyMap, the APEX, and the CASI datasets, respectively. Moreover, this method showed better efficiency in comparison to the spectralbased classifications because the OAs of the proposed method was 5.67 and 3.75% higher than the conventional RFC and SVM classifiers, respectively.